A closed water recirculation system for ecological studies in marine fish larvae: growth and survival of sea bass larvae fed with live prey

2000 ◽  
Vol 13 (1) ◽  
pp. 29-35 ◽  
Author(s):  
M Pilar Olivar
Keyword(s):  
Fish Larvae ◽  
Sea Bass ◽  
Ecological Studies ◽  
Live Prey ◽  
Growth And Survival ◽  
Marine Fish Larvae ◽  
Recirculation System ◽  
Water Recirculation ◽  
Closed Water ◽  
Sea Bass Larvae

scholarly journals Effect of nature of dietary lipids on European sea bass morphogenesis: implication of retinoid receptors

2005 ◽  
Vol 94 (6) ◽  
pp. 877-884 ◽  
Author(s):  
Laure Villeneuve ◽  
Enric Gisbert ◽  
Jose L. Zambonino-Infante ◽  
Patrick Quazuguel ◽  
Chantal L. Cahu
Keyword(s):  
Retinoic Acid ◽  
Neutral Lipid ◽  
Retinoic Acid Receptor ◽  
Sea Bass ◽  
Dietary Lipids ◽  
European Sea Bass ◽  
Growth And Survival ◽  
Acid Receptor ◽  
Epa And Dha ◽  
Sea Bass Larvae

The effect of the nature and form of supply of dietary lipids on larval development was investigated in European sea bass larvae, by considering the expression of several genes involved in morphogenesis. Fish were fed from 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets incorporating different levels of EPA and DHA provided by phospholipid or neutral lipid. Phospholipid fraction containing 1·1 % (PL1 diet) to 2·3 % (PL3 diet) of EPA and DHA sustained good larval growth and survival, with low vertebral and cephalic deformities. Similar levels of EPA and DHA provided by the neutral lipid fraction were teratogenic and lethal. Nevertheless, dietary phospholipids containing high levels of DHA and EPA (PL5 diet) induced cephalic (8·5 %) and vertebral column deformities (35·3 %) adversely affecting fish growth and survival; moreover, a down-regulation of retinoid X receptor α (RXRα), retinoic acid receptor α, retinoic acid receptor γ and bone morphogenetic protein-4 genes was also noted in PL5 dietary group at day 16. High levels of dietary PUFA in neutral lipid (NL3 diet) first up-regulated the expression of RXRα at day 16 and then down-regulated most of the studied genes at day 23, leading to skeletal abnormalities and death of the larvae. A moderate level of PUFA in neutral lipids up-regulated genes only at day 16, inducing a lesser negative effect on growth, survival and malformation rate than the NL3 group. These results showed that retinoid pathways can be influenced by dietary lipids leading to skeletal malformation during sea bass larvae development.

scholarly journals Effect of dietary phospholipid level and phospholipid:neutral lipid value on the development of sea bass (Dicentrarchus labrax) larvae fed a compound diet

2003 ◽  
Vol 90 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Chantal L. Cahu ◽  
José L. Zambonino Infante ◽  
Valérie Barbosa
Keyword(s):  
Phospholipase A2 ◽  
Pancreatic Lipase ◽  
Fish Larvae ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Phospholipid Content ◽  
Transcriptional Level ◽  
Good Growth ◽  
Live Prey ◽  
Larval Quality

The aim of the study was to determine the influence of dietary phospholipid concentration on survival and development in sea bass (Dicentrarchus labrax) larvae. Larvae were fed from day 9 to day 40 post-hatch with an isoproteic and isolipidic formulated diet with graded phospholipid levels from 27 to 116 g/kg DM and different phospholipid:neutral lipid values. The best growth (32 mg at the end of the experiment) survival (73 %) and larval quality (only 2% of malformed larvae) were obtained in the larvae fed the diet containing 116 g phospholipid/kg DM (P < 0·05). These results were related to the amount of phosphatidylcholine and phosphatidylinositol included in this diet (35 and 16 g/kg respectively). Amylase, alkaline phosphatase and aminopeptidase N activities revealed a proper maturation of the digestive tract in the two groups fed the highest phospholipid levels. Regulation of lipase and phospholipase A2 by the relative amount of their substrate in the diet occurred mainly at the transcriptional level. The response of pancreatic lipase to dietary neutral lipid was not linear. As in mammals 200 g triacylglycerol/kg diet seems to represent a threshold level above which the response of pancreatic lipase is maximal. The response of phospholipase A2 to dietary phospholipid content was gradual and showed a great modulation range in expression. Sea bass larvae have more efficient capacity to utilize dietary phospholipid than neutral lipids. For the first time a compound diet sustaining good growth, survival and skeletal development has been formulated and can be used in total replacement of live prey in the feeding sequence of marine fish larvae.

Size-dependent foraging of larval Atlantic cod (Gadus morhua)

2004 ◽  
Vol 82 (8) ◽  
pp. 1380-1389 ◽  
Author(s):  
Velmurugu Puvanendran ◽  
Karine Salies ◽  
Benjamin Laurel ◽  
Joseph A Brown
Keyword(s):  
Marine Fish ◽  
Gadus Morhua ◽  
Atlantic Cod ◽  
Prey Selection ◽  
Fish Larvae ◽  
Growth And Survival ◽  
Size Dependent ◽  
Marine Fish Larvae ◽  
Key Factor ◽  
Foraging Environment

Most marine fish larvae are thought to be gape-limited predators, and the presence of suitably sized prey at the appropriate time in the foraging environment is a key factor for their growth and survival. Two experiments were carried out: in experiment 1, we investigated feeding of Atlantic cod (Gadus morhua L., 1758) larvae from 5 to 35 days post hatch on prey of three different sizes: two different-sized strains of rotifers, small rotifers (SR) and large rotifers (LR), and Artemia Leach, 1819 nauplii (AN), or an equal mixture of the three types of prey (MIX). In experiment 2, cod larvae were fed SR, LR, or a combination of SR and LR (MIX-R) at concentrations of 1500 and 4000 prey·L–1 from 4 to 20 days post hatch. Feeding incidence, number of prey in the gut, mouth gape, and prey selection were measured. In experiment 1, feeding incidence was initially higher in the SR treatment, while larvae failed to start feeding in either the LR or the AN treatment at 5 dph. Larvae in the LR treatment started feeding at 8 dph, and feeding incidence was comparable to that in the SR treatment, but the total number of prey eaten was higher in the SR than in the LR treatment until 20 dph. Larvae did not start feeding on AN until 26 dph, although they attacked them from 5 dph. In the MIX-R treatment in experiment 2, larvae fed selectively on LR at 4000 prey·L–1, whereas no selection was observed at 1500 prey·L–1. Our results suggest that cod larvae are gape-limited predators and the concentration of prey affects prey selection.

Incidence and Composition of Calculi in the Urinary Bladder of Intensively Reared Marine Fish Larvae

1996 ◽  
Vol 47 (2) ◽  
pp. 255 ◽  
Author(s):  
BF Nowak ◽  
SC Battaglene
Keyword(s):  
Marine Fish ◽  
Electron Probe ◽  
Fish Larvae ◽  
Research Centre ◽  
Live Prey ◽  
Marine Fish Larvae ◽  
Wide Range ◽  
Pagrus Auratus ◽  
Electron Probe Microanalyser ◽  
Intensive Rearing

Calculi were observed in the urinary tracts of a wide range of intensively reared marine fish larvae at the Port Stephens Research Centre. Data are presented for three species: snapper Pagrus auratus, mulloway Argyrosomus hololepidotus, and Australian bass Macquaria novemaculeata. Calculi first appeared in P. auratus on Day 5 after hatching, in M. novemaculeata on Day 11, and in A. hololepidotus on Day 12. Around 80% of larvae typically had calculi after two weeks of intensive rearing. No relationship could be found between the absence of the functional swim bladder and the presence of calculi in all species studied. The presence of calculi did not stop the larvae of any species from actively feeding on live prey. Calculi from M. novemaculeata and P. auratus were analysed with an electron probe microanalyser. Phosphorus and calcium were detected in all calculi, usually accompanied by magnesium. Potential causes for the formation of calculi are discussed.

scholarly journals Effect of enriching live feeds with HUFA on growth and survival of clownfish Amphiprion ocellaris (Cuvier, 1830) larvae

2019 ◽  
Vol 19 (4A) ◽  
pp. 191-199
Author(s):  
Ho Son Lam ◽  
Nguyen Thi Nguyet Hue ◽  
Dinh Truong An ◽  
Phan Thi Khanh
Keyword(s):  
Survival Rate ◽  
Production Efficiency ◽  
Specific Growth ◽  
Fish Larvae ◽  
Control Diet ◽  
Live Prey ◽  
Growth And Survival ◽  
Live Feed ◽  
Live Feeds ◽  
Set Up

The aims of this study was to evaluate the effect of HUFA-enriched live feed in rearing Nemo fish larvae (Amphiprion ocellaris), 5 treatments were set up (each treatment was repeated in triplicate) with 5 concentrations of HUFA (Super Selco) (0, 50, 100, 150 and 200 ppm). After 45 days of culture with HUFA-enriched live feeds of different concentrations, the total length-TL as well as the specific growth rate (SGRL) in body length and survival rate of Nemo fish larvae in the treatments had significant differences (p < 0.05). In the enriched live prey diet of 100 ppm Selco, TL, SGRL and the survival rate of Nemo fish larvae were the highest (10.01 ± 0.150 mm, 7.20 ± 0.099%/day and 82.67 ± 0.881%, respectively). Lowest TL, SGRL and survival rate were obtained in larvae fed with the control diet (8.65 ± 0.051 mm, 6.23 ± 0.041%/day and 68.70 ± 0.881%, respectively). The results of this experiment suggest that the optimal Selco concentration used to enrich live feeds for Nemo fish larvae is 100 ppm, which can improve the production efficiency of Nemo stock.

scholarly journals The Inhibitory Effects and Positive Contributions of Live Foods on Protease Activities of Meagre, Argyrosomus regius (Asso 1801) Larvae in vitro Assay

2018 ◽  
Author(s):  
Gürkan Diken ◽  
Orhan Demir ◽  
Mehmet Naz
Keyword(s):  
Marine Fish ◽  
Fish Larvae ◽  
Survival Ratio ◽  
Inhibitory Effects ◽  
Growth And Survival ◽  
In Vitro Techniques ◽  
Artemia Nauplii ◽  
Marine Fish Larvae ◽  
Argyrosomus Regius

The aim of this study was to gather preliminary data about the potential inhibitory effects and contributions of live foods used from 3 to 32 days after hatching (DAH) in routine feeding protocols on protease activities of meagre, Argyrosomus regius (Asso 1801) larvae, using in vitro techniques. Enriched rotifer, Artemia nauplii and Artemia metanauplii were tested in the present study. The highest values of protease activities of meagre larvae at 7 DAH in 2013 and 2014 years were established. The lowest values at 15 DAH in 2013 and at 20 DAH in 2014 were observed. Protease activities of enriched rotifer, Artemia nauplii, and Artemia metanauplii were 21.76&plusmn;0.31, 36.00&plusmn;1.48&ndash;29.33&plusmn;0.93, and 416.44&plusmn;19.7&ndash;403.53&plusmn;11.85 U/mg protein, respectively (p&lt; 0.05). The highest inhibitions of live foods were observed at 7 DAH. The positive contributions of live food Artemia metanauplii&rsquo;s on protease activities of meagre larvae were significant (p&lt; 0.05). The inhibitory effects and positive contributions of live foods on survival and growth rates of meagre larvae, should be taken into account in meagre and other marine fish larvae&rsquo;s future studies. Cysteine protease activities of Artemia sp. should be investigated to provide the higher growth and survival ratio from the feeding protocols used in marine fish larvae.

scholarly journals The Inhibitory Effects and Positive Contributions of Live Foods on Protease Activities of Meagre, Argyrosomus regius (Asso 1801) Larvae in vitro Assay

2018 ◽  
Author(s):  
Gürkan Diken ◽  
Orhan Demir ◽  
Mehmet Naz
Keyword(s):  
Marine Fish ◽  
Fish Larvae ◽  
Survival Ratio ◽  
Inhibitory Effects ◽  
Growth And Survival ◽  
In Vitro Techniques ◽  
Artemia Nauplii ◽  
Marine Fish Larvae ◽  
Argyrosomus Regius

The aim of this study was to gather preliminary data about the potential inhibitory effects and contributions of live foods used from 3 to 32 days after hatching (DAH) in routine feeding protocols on protease activities of meagre, Argyrosomus regius (Asso 1801) larvae, using in vitro techniques. Enriched rotifer, Artemia nauplii and Artemia metanauplii were tested in the present study. The highest values of protease activities of meagre larvae at 7 DAH in 2013 and 2014 years were established. The lowest values at 15 DAH in 2013 and at 20 DAH in 2014 were observed. Protease activities of enriched rotifer, Artemia nauplii, and Artemia metanauplii were 21.76&plusmn;0.31, 36.00&plusmn;1.48&ndash;29.33&plusmn;0.93, and 416.44&plusmn;19.7&ndash;403.53&plusmn;11.85 U/mg protein, respectively (p&lt; 0.05). The highest inhibitions of live foods were observed at 7 DAH. The positive contributions of live food Artemia metanauplii&rsquo;s on protease activities of meagre larvae were significant (p&lt; 0.05). The inhibitory effects and positive contributions of live foods on survival and growth rates of meagre larvae, should be taken into account in meagre and other marine fish larvae&rsquo;s future studies. Cysteine protease activities of Artemia sp. should be investigated to provide the higher growth and survival ratio from the feeding protocols used in marine fish larvae.

Marine Fish Larvae Feeding: Formulated Diets or Live Prey?

1993 ◽  
Vol 24 (2) ◽  
pp. 211-224 ◽  
Author(s):  
Jeannine People Le Ruyet ◽  
Jean Claude Alexandre ◽  
Laurence Thébaud ◽  
Chantal Mugnier
Keyword(s):  
Marine Fish ◽  
Fish Larvae ◽  
Live Prey ◽  
Formulated Diets ◽  
Marine Fish Larvae

Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida)

2013 ◽  
Vol 1 (2) ◽  
pp. 143
Author(s):  
Petrus P Letsoin ◽  
Henneke Pangkey ◽  
Julius Sampekalo ◽  
Inneke F.M Rumengan ◽  
Stenly Wullur ◽  
...  
Keyword(s):  
Body Size ◽  
Marine Fish ◽  
Fish Larvae ◽  
Nannochloropsis Oculata ◽  
Tropical Fish ◽  
Sampling Location ◽  
Larval Rearing ◽  
Brachionus Rotundiformis ◽  
Marine Fish Larvae ◽  
Significant Difference

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae.  Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.

Sign in / Sign up

Export Citation Format

Share Document