In vitro selection of herbicide resistance in microspore-derived embryos of oilseed rape (Brassica napus L.)

2006 ◽  
Vol 3 (1) ◽  
pp. 33-38
Author(s):  
Xu Ling ◽  
Zhang Guo-Qing ◽  
Gu Hong-Hui ◽  
He Yun ◽  
Zhou Wei-Jun
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Chromosome Doubling ◽  
Microspore Culture ◽  
Brassica Napus L ◽  
Regenerated Plants ◽  
Short Time ◽  
Selection Of

AbstractUsing four oilseed rape (Brassica napus L.) F1 hybrids (7039, 7040, 282 and 5102) as donor plants for microspore culture, the experiment was conducted to select glyphosate- and haloxyfop-resistant embryos through application of these substances to the cultural media with microspore-derived embryos in vitro. Genotypes 7039 and 7040 were used to select glyphosate-resistant regenerated plants, and genotypes 282 and 5102 to select haloxyfop-resistant plants. The embryos at cotyledonary stage were grown on glyphosate- and haloxyfop-containing MS-2 medium for 2 weeks. The non-resistant embryos collapsed after a short time, while the resistant ones turned green and survived for 2 weeks. Transferred into the normal MS-2 medium for further plant regeneration, the regenerated plants from green embryos showed tolerance to 0.25% sprayed glyphosate, indicating the effectiveness and reliability of this in vitro selection method. When the regenerated plants selected from 0.02% haloxyfop were sprayed with 0.05% haloxyfop, most of them grew well; however, the survival rate of the regenerated plants from 0.01% haloxyfop-containing medium was lower. The present experiment indicates that the use of 0.02% haloxyfop in the selection of haloxyfop-resistant plants was more promising than that of 0.01%. The chromosome doubling efficiency of regenerated plants reached 34% and 52% after being treated with 170 mg/l colchicine for 20 and 30 h, respectively.

scholarly journals Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos

2019 ◽  
Vol 55 (No. 4) ◽  
pp. 162-165
Author(s):  
Miroslav Klíma ◽  
Eva Jozová ◽  
Irena Jelínková ◽  
Vratislav Kučera ◽  
Shengwu Hu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Restorer Gene ◽  
Brassica Napus L ◽  
Rf Gene ◽  
Dh Lines ◽  
Non Destructive ◽  
Selection Of

Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype comparisons. In 14 plants (12.2%) the phenotype did not match the genotype: two plants were marked as fertile, but without the Rf gene. Vice versa, in 12 sterile plants the Rf gene was determined. Nevertheless, the two fertile plants did not produce any pods after self-pollination. In total, 47 doubled haploid (DH) lines with good seed yield, carrying the Rf gene were harvested.  

scholarly journals Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)

2015 ◽  
Vol 18 (1) ◽  
pp. 58-60 ◽  
Author(s):  
Lenka Havlíčková ◽  
Miroslav Klíma ◽  
Marie Přibylová ◽  
Alois Antonín Hilgert-Delgado ◽  
Vratislav Kučera ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Winter Oilseed Rape ◽  
Fertility Restorer ◽  
Brassica Napus L ◽  
Fertility Restorer Gene ◽  
Non Destructive ◽  
Selection Of

scholarly journals Establishment of glyphosate selective concentrations for winter rapeseed (Brassica napus L.) transgenic tissues efficient selection in vitro

2019 ◽  
Vol 25 ◽  
pp. 197-201
Author(s):  
I. S. Hnatiuk ◽  
O. I. Varchenko ◽  
M. F. Parii ◽  
Yu. V. Symonenko
Keyword(s):  
Brassica Napus ◽  
Nutrient Medium ◽  
In Vitro Selection ◽  
Brassica Napus L ◽  
Regeneration Stage ◽  
Winter Rapeseed ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Efficient Selection ◽  
Selection Of

Aim. To establish the optimal concentration of glyphosate for efficient selection of transgenic tissue culture in biotechnology of rapeseed by in vitro selection of glyphosate-resistant and non-resistant winter rape. Methods. As an explants 7–10 mm fragments of 6-day rapeseed hypocotyls were used to cultivate on the medium, supplemented with 1 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) for 12 days at 24°C under dark conditions for the initiation of callusogenesis. For regeneration of plants, the MS nutrient medium was supplemented with 3 mg/L of 6-benzylaminopurine (BAP), 2 mg/L of zeatin, 0.1, 0.5 and 1 mM of glyphosate, respectively. As a control, glyphosate-free regenerative nutrient medium was used. The results were statistically processed using Microsoft Excel. Results. It has been shown that glyphosate-susceptible rape explants form leaves and shoots with a frequency up to 8.3 % ± 0.8 % on the medium supplemented with 0.1 mM glyphosate compared to 57.2 % ± 0.6 % in the control. However, the morphogenic structures did not pass selection after transferring shoots to the elongation medium supplemented with 0.1 mM glyphosate. Conclusions. For the in vitro selection of rapeseed 0.1 mM glyphosate can be used in a nutrient medium at the stage of adventitious buds formation. But at the regeneration stage, the amount of herbicide should be increased to avoid false-positive results. Keywords: winter rapeseed, Brassica napus L., in vitro culture, selective marker, herbicide, glyphosate.

The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts

1988 ◽  
Vol 7 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Eric B. Swanson ◽  
Marc P. Coumans ◽  
Gerry L. Brown ◽  
Jayanti D. Patel ◽  
W. D. Beversdorf
Keyword(s):  
Brassica Napus ◽  
In Vitro Selection ◽  
Brassica Napus L ◽  
Tolerant Plants ◽  
Herbicide Tolerant ◽  
Selection Of

scholarly journals Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0233959 ◽  
Author(s):  
Stanisław Spasibionek ◽  
Katarzyna Mikołajczyk ◽  
Hanna Ćwiek–Kupczyńska ◽  
Teresa Piętka ◽  
Krystyna Krótka ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Marker Assisted Selection ◽  
Inbred Lines ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
High Oleic ◽  
Low Linolenic ◽  
Selection Of

scholarly journals Utilisation of doubled haploids in winter oilseed rape (Brassica napus L.) breeding

2012 ◽  
Vol 38 (No. 1) ◽  
pp. 50-54 ◽  
Author(s):  
V. Kučera ◽  
M. Vyvadilová ◽  
M. Klíma
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
The Czech Republic ◽  
Yield Parameters ◽  
The World

A survey of development and prospects of the utilisation of doubled haploid techniques in rapeseed breeding in the world and in the Czech Republic is presented. The first utilisation of spontaneously occurred haploids from Brassica napus inbreeding programmes is described. The development of techniques of anther and later microspore culture is outlined. Special emphasis is given to the practical use of doubled haploids for the improvement of the effectiveness of breeding new productive cultivars. Some partial results of evaluation of yield parameters and resistance to important diseases in the obtained doubled haploid lines of winter oilseed rape are shown. The literary review and present results indicate, that the doubled haploid technique can be effectively used for the development of homozygous oilseed rape lines as an alternative to conventional methods.

scholarly journals The Impact of Forest Fungi on Promoting Growth and Development of Brassica napus L.

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2475
Author(s):  
Grażyna B. Dąbrowska ◽  
Zuzanna Garstecka ◽  
Alina Trejgell ◽  
Henryk P. Dąbrowski ◽  
Wiktoria Konieczna ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Growth And Development ◽  
Plant Pathogens ◽  
Fungal Species ◽  
Brassica Napus L ◽  
Number Of Microorganisms ◽  
The Impact

Inoculation of plants with fungi has been shown to increase yields by improving germination, seedling vigor, plant growth, root morphogenesis, photosynthesis, and flowering through direct or indirect mechanisms. These mechanisms include solubilization and mineralization of nutrients, facilitating their uptake by plants, regulation of hormone balance, production of volatile organic compounds and microbial enzymes, suppression of plant pathogens, and mitigation of abiotic stresses. In the presented experiments, the effect of selected forest soil fungi on the growth and development of Brassica napus L. seedlings was investigated. Inoculation was carried out in vivo and in pot experiments with ectomycorrhizal and saprophytic fungi typical of forest soils: Collybia tuberosa, Clitocybe sp., Laccaria laccata, Hebeloma mesophaeum, and Cyathusolla. It was shown that all analyzed fungi produced IAA. In the in vitro experiment, B. napus inoculated with L. laccata showed stimulated root growth and greater number of leaves compared to control plants. A similar stimulatory effect on lateral root formation was observed in cuttings grown in pots in the presence of the C. olla fungus. In the pot experiment, the seedlings inoculated with the L. laccata fungus also showed increased growth of shoots and biomass. The effect of inoculation with the tested fungal strains, especially C. olla, on the growth and development of oilseed rape was probably indirect, as it also contributed to an increase in the number of microorganisms, especially soil bacteria. The expression of the metallothioneins in B. napus (BnMT1-BnMT3) varied depending on the fungal species. The presence of C. olla significantly increased BnMT2 expression in oilseed rape. It was found that BnMT1 expression increased and BnMT3 transcripts decreased in plants growing in the presence of L. laccata. This indicates the involvement of BnMT in the adaptation of oilseed rape to growth in fungi presence.

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