scholarly journals Shaping of the fungal communities isolated from yellow lupin seeds (Lupinus luteus L.) throughout storage time

2012 ◽  
Vol 58 (2) ◽  
pp. 407-416
Author(s):  
Bożena Cwalina-Ambroziak ◽  
Tomasz P. Kurowski
Keyword(s):  
Crop Rotation ◽  
Storage Time ◽  
Colletotrichum Gloeosporioides ◽  
Lupinus Luteus ◽  
Yellow Lupin ◽  
Storage Duration ◽  
Rhizopus Nigricans ◽  
Lupin Seeds ◽  
Traditional Cultivars ◽  
Yellow Lupine

The object of the experiment were seeds of two traditional cultivars of yellow lupin (Juno and Amulet) cultivated in 1999 in two crop-rotation with 20% and 33% yellow lupine contribution. The quantitative and qualitative composition of the fungal community colonizing the seeds were determined in the laboratory conditions after 0.5-, 1.5- and 2.5-year of storage time. In total 1077 fungal colonies were isolated from the lupin seeds. Fungi representing the species of <i>Penicillium</i> - 29.3%, <i>Alternaria alternata</i> - 26.7% and <i>Rhizopus nigricans</i> - 12.7% were isolated most widely. Among the fungi pathogenic to lupin, the species of <i>Colletotrichum gloeosporioides</i> (16.3% isolates) was dominant. The crop rotation with 20% lupin reduced the number of fungal colonies colonizing the seeds including the pathogens from the species of <i>C. gloeosporioides</i>. Seed disinfection decreased the total number of fungal colonies isolated from both cultivars. Higher number of <i>C. gloeosporioides</i> isolates was found in the combination with disinfected seeds. More fungal colonies were obtained from seeds of cv. Amulet than from those of cv. Juno. The storage duration had an effect on the population and the composition of species of fungi isolated from seeds of yellow lupine. With longer storage population of <i>Penicillium</i> spp. and <i>Rhizopus</i> spp. increased, whereas the population of <i>C. gloeosporioides</i> decreased.

scholarly journals Adenosine 5′-tetraphosphate phosphohydrolase from yellow lupin seeds: purification to homogeneity and some properties

1997 ◽  
Vol 328 (1) ◽  
pp. 257-262 ◽  
Author(s):  
Andrzej GURANOWSKI ◽  
Elżbieta STARZYŃSKA ◽  
Paul BROWN ◽  
G. Michael BLACKBURN
Keyword(s):  
Divalent Cation ◽  
Polypeptide Chain ◽  
Lupinus Luteus ◽  
Nucleoside Triphosphate ◽  
Yellow Lupin ◽  
Ph Optimum ◽  
Single Polypeptide Chain ◽  
Lupin Seeds ◽  
Single Polypeptide ◽  
Hydrolysis Of

Adenosine 5ʹ-tetraphosphate phosphohydrolase (EC 3.6.1.14) has been purified to homogeneity from the meal of yellow lupin (Lupinus luteus) seeds. The enzyme is a single polypeptide chain of 25±1 kDa. It catalyses the hydrolysis of a nucleoside 5ʹ-tetraphosphate to a nucleoside triphosphate and orthophosphate, and hydrolysis of tripolyphosphate but neither pyrophosphate nor tetraphosphate. A divalent cation, Mg2+, Co2+, Ni2+ or Mn2+, is required for these reactions. The pH optimum for hydrolysis of adenosine 5ʹ-tetraphosphate (p4A) is 8.2, Vmax is 21±1.7 μmol/min per mg of protein and the Km for p4A is 3±0.6 μM. At saturating p4A concentrations, the rate constant for the reaction is 8.5±0.7 s-1 [at 30 °C, in 50 mM Hepes/KOH (pH 8.2)/5 mM MgCl2/0.1 mM dithiothreitol]. p4A and guanosine 5ʹ-tetraphosphate are hydrolysed at the same rate. Adenosine 5ʹ-pentaphosphate (p5A) is degraded 1/200 as fast and is converted into ATP and two molecules of orthophosphate, which are liberated sequentially. This contrasts with the cleavage of p5A by the lupin diadenosine tetraphosphate hydrolase (EC 3.6.1.17), which gives ATP and pyrophosphate. Zn2+, F- and Ca2+ ions inhibit the hydrolysis of p4A with I50 values of 0.1, 0.12 and 0.2 mM respectively.

scholarly journals The Loss of Vigour and Sowing Value of Yellow Lupin Seeds (Lupinus luteus L.) as a Result of Mechanical Harvesting

2016 ◽  
Vol 73 (1) ◽  
pp. 53-62
Author(s):  
Agnieszka Faligowska ◽  
Grażyna Szymańska ◽  
Katarzyna Panasiewicz
Keyword(s):  
Electrical Conductivity ◽  
Seed Quality ◽  
Pearson Correlation ◽  
Mean Value ◽  
Lupinus Luteus ◽  
Pearson Correlation Coefficient ◽  
Yellow Lupin ◽  
Lupin Seeds ◽  
The Mean

AbstractThe aim of the study was to determine the effect of mechanical harvest on the seed quality of yellow lupin. Two effects were studied: the cultivar of yellow lupin (the indeterminate cultivar - Mister and the determinate cultivar - Perkoz) and harvest methods: hand-picked plants with manual shelling of seeds as a control and mechanical shelling with a plot harvester. In comparison with manual shelling of seeds, the mechanical harvest reduced the seed germination and increased the number of abnormal seeds both cultivars. Determinate cultivar was more sensitive, because the loss of its quality was higher (germination of 10%) than indeterminate cultivar (6%). Perkoz had also higher electrical conductivity, with the mean value of 34.3 μS × cm−1× g−1. Manual shelled seeds were characterized by a significantly lower leakage of exudates (24.9 μS × cm−1× g−1), and hence, it exhibited greater vigour than mechanical harvested seeds. Most relations of Pearson correlation coefficient between vigour tests and germination were strong or practically functional.

Chemical and Nutritional Evaluation of Protein-Rich Ingredients Obtained through a Technological Process from Yellow Lupin Seeds (Lupinus luteus)

2019 ◽  
Vol 74 (4) ◽  
pp. 508-517 ◽  
Author(s):  
César Burgos-Díaz ◽  
Mauricio Opazo-Navarrete ◽  
Traudy Wandersleben ◽  
Monserrat Soto-Añual ◽  
Tamara Barahona ◽  
...  
Keyword(s):  
Technological Process ◽  
Lupinus Luteus ◽  
Yellow Lupin ◽  
Nutritional Evaluation ◽  
Lupin Seeds ◽  
Protein Rich

scholarly journals Sanitary state of yellow lupine (Lupinus luteus L.) in different agricultural conditions

2012 ◽  
Vol 58 (2) ◽  
pp. 395-406
Author(s):  
Tomasz P. Kurowski ◽  
Tadeusz Bieniaszewski ◽  
Edyta Jaźwińska
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Plant Density ◽  
Weather Conditions ◽  
Vegetation Period ◽  
Lupinus Luteus ◽  
Injury Index ◽  
Black Leg ◽  
Yellow Lupine ◽  
Serious Disease ◽  
Sanitary State

In 1999-2001 the effect of sowing method and plant density on the sanitary state of three cultivars of yellow lupine protected with fungicides against diseases was investigated. In spring of 2000 the seedlings on the experimental fields generally withered, which was most likely caused by the applied herbicide, however in 1999 and 2001 seedling black leg (complex of fungi) was reported. The plants germinating from the seeds sown in points were clearly less attacked by pathogens evoking seedling black leg than those sown in rows. The most serious disease of yellow lupine was antracnose (<i>Colletotrichum gloeosporioides</i>). It occurred in all experimental years and its intensity increased during vegetation period. Cultivars of lupine used to the experiment were attacked by <i>Colletotrichum gloeosporioides</i> in a different degree, however, the injury index was mainly determined by weather conditions and the lupine development phase. The applied fungicides significantly limited the development of lupine antracnose. The effect of sowing method and varied plant density on disease intensity was varied.

scholarly journals The effect of addition of yellow lupin seeds (Lupinus luteus L.) to laying hen diets on performance and egg quality parameters

2017 ◽  
Vol 26 (3) ◽  
pp. 247-256 ◽  
Author(s):  
A. Rutkowski ◽  
M. Hejdysz ◽  
S. Kaczmarek ◽  
M. Adamski ◽  
S. Nowaczewski ◽  
...  
Keyword(s):  
Egg Quality ◽  
Quality Parameters ◽  
Laying Hen ◽  
Lupinus Luteus ◽  
Yellow Lupin ◽  
Lupin Seeds

scholarly journals ESTIMATION OF EST-SSR AND SRAP-RGA MARKERS FOR GENOTYPING YELLOW LUPIN (LUPINUS LUTEUS L.)

2018 ◽  
Vol 63 (3) ◽  
pp. 298-306
Author(s):  
E. N. Sysoliatin ◽  
N. V. Anisimova ◽  
O. G. Babak ◽  
V. S. Anokhina ◽  
I. Y. Romanchuk ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Lupinus Luteus ◽  
Yellow Lupin ◽  
Yellow Lupine

14 SRAP-RGA primer combinations and 8 EST-SSR markers were tested on the collection of 10 different yellow lupine samples. The most efficient primers for intravarietal differentiation of yellow lupine were distinguished according their parameters. Reliable correlations were determined between the presence of EST-SSR fragments and some characteristics of productivity in hybrid (F2 ) yellow lupine populations. 

scholarly journals Results of the introduction of Lupinus varieties and samples in the Steppe Crimea

2020 ◽  
Author(s):  
O.P. Ptashnik ◽  
Keyword(s):  
Grain Size ◽  
Seed Yield ◽  
Lupinus Albus ◽  
Growing Season ◽  
Lupinus Luteus ◽  
White Lupin ◽  
Average Yield ◽  
Yellow Lupin ◽  
Lupin Seeds ◽  
Lupinus Albus L

Within a framework of Lupinus varieties and samples assessment, we have found that the growing season of Lupinus albus L. was 93, Lupinus angustifolius L. – 99, and Lupinus luteus L. – 95 days under conditions of the Steppe Crimea. The average yield of white lupin seeds was 1.63 t/ha; blue or narrow-leafed lupin – 1.18 t/ha; yellow lupin – 0.72 t/ha. L. albus is more productive compared to L. angustifolius and L. luteus. The seed yield of all studied varieties and samples of white lupin was higher than that of the standard one ‘Michurinsky’. Samples CH-2-17 and CH-78-16 were the most high-yielding (1.77 and 1.74 t/ha, respectively). Variety ‘Belorozovy 144’ was the most promising among the representatives of narrow-leafed lupin; its yield reached 1.64 t/ha. The best in grain size was white lupin; 1000-grains weight was within the range of 200-222g. Varieties of narrowleafed lupin ‘Belorozovy 144’ and ‘Bryansky kormovoy’ contained the least amount of alkaloids (0.021 and 0.022%, respectively).

scholarly journals Studies on soluble carbohydrates in yellow lupin (Lupinus luteus L.) seeds of different age

2011 ◽  
Vol 76 (4) ◽  
pp. 309-312 ◽  
Author(s):  
Kazimierz Zalewski ◽  
Lesław B. Lahuta
Keyword(s):  
Seed Storage ◽  
Dry Mass ◽  
Lupinus Luteus ◽  
Soluble Carbohydrates ◽  
Mass Ratio ◽  
Yellow Lupin ◽  
Embryonic Axes ◽  
Laboratory Conditions ◽  
Lupin Seeds

Yellow lupin seeds cv. Juno were stored under laboratory conditions for 2 month, 4, 6 and 8 years. Eighteen soluble carbohydrates were identified in embryonic axes and cotyledons of different age seeds. The concentration of soluble carbohydrates in analysed seeds ranged from 25 to 34% of dry mass. Axes contained more carbohydrates than cotyledons. Stachyose dominated in axes, and verbascose - in cotyledons. Other detected galactosides were: galactinol, galactosyl pinitols and galactosyl chiro-inositols (fagopyritols), but their content was several-fold lower than that of RFOs (in both axes and cotyledons tissues). The concentration of soluble carbohydrates indicated, that sucrose to RFOs mass ratio, or other changes in sugars composition are not indicators of seed storage.

Soluble sugars and flatulence-producing oligosaccharides in maturing yellow lupin (Lupinus luteus L.) seeds

1997 ◽  
Vol 7 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Ryszard J. Górecki ◽  
Agnieszka Piotrowicz-Cieślak ◽  
Ralph L. Obendorf
Keyword(s):  
Soluble Sugars ◽  
Dry Mass ◽  
Fresh Mass ◽  
Lupinus Luteus ◽  
Yellow Lupin ◽  
Seed Germinability ◽  
Seed Desiccation ◽  
Seed Growth ◽  
Lupin Seeds ◽  
Mature Seeds

AbstractThe flatulence-producing soluble oligosaccharides are an important component of lupin seeds and were assayed to establish the pattern of their accumulation in relation to germinability during seed development and maturation. Maturing yellow lupin cv. Juno seeds were harvested at 5-day intervals from 15 to 45 days after flowering (DAF). Seed fresh mass increased to a maximum at 35 DAF followed by a decrease when axis and cotyledon tissues changed colour from green to yellow. Maximum seed fresh mass corresponded to the maximum seed size. Seed dry mass continuously increased until 40 DAF. About 75% of mature seed dry mass was in cotyledons, 22% in testa, and 3% in axis. Maximum seed germinability occurred at 45 DAF after maximum seed dry mass and desiccation. Maturing yellow lupin seeds were desiccation tolerant. Mature dry seeds contained 10.9% oligosaccharides and 1.5% sucrose. During seed growth stachyose accumulation preceded accumulation of raffinose and verbascose. The highest rate of oligosaccharide accumulation appeared during seed desiccation and correlated with the acquisition of the ability to germinate. The sucrose:oligosaccharide ratio continuously decreased reaching 0.13 in mature seeds. Accumulation of oligosaccharides in maturing yellow lupin seeds is associated with seed germinability and seed desiccation tolerance.

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