Detection in human sera of IgG, IgM and IgA to excreted/secreted antigens fromToxoplasma gondiiby use of dot-ELISA and immunoblot assay

SummaryWe have observed that naturally occurring serum antibodies generated a 30 Kd band in a platelet immunoblot assay. The target protein had the same molecular weight (30 Kd) under nonreduced and reduced electrophoretic conditions, and could be immunoblotted from either autologous or homologous platelet lysates. Also, the 30 Kd reactive autoantibodies could be totally adsorbed by platelet cytoskeletons. From these data one likely candidate for the autoantibody target was the intracellular platelet protein tropomyosin. Indeed, a commercially available monoclonal antitropomyosin antibody reacted with proteins comigrating with this 30 Kd band; affinity purified human platelet tropomyosin was bound by the antibodies that recognized the 30 Kd protein. This body of evidence conclusively demonstrated that naturally occurring serum autoantibodies reacted with the platelet cytoskeleton protein - tropomyosin. These tropomyosin specific antibodies were found in roughly the same percentage of sera from patients with chronic idiopathic thrombocytopenic purpura (ITP) as from normal individuals.

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Abnormal Processing of the Glycoprotein IIb Transcript due to a Nonsense Mutation in Exon 17 Associated with Glanzmann’s Thrombasthenia

Thrombosis and Haemostasis ◽

10.1055/s-0038-1653864 ◽

1995 ◽

Vol 73 (05) ◽

pp. 756-762 ◽

Cited By ~ 25

Author(s):

Yoshiaki Tomiyama ◽

Hirokazu Kashiwagi ◽

Satoru Kosugi ◽

Masamichi Shiraga ◽

Yoshio Kanayama ◽

...

Keyword(s):

Dna Analysis ◽

Molecular Genetic ◽

Genetic Defect ◽

Nucleotide Sequence Analysis ◽

Type I ◽

Normal Amount ◽

Immunoblot Assay ◽

Glanzmann’S Thrombasthenia ◽

Glanzmann's Thrombasthenia ◽

Pcr Products

SummaryWe analyzed the molecular genetic defect responsible for type I Glanzmann’s thrombasthenia in a Japanese patient. In an immunoblot assay using polyclonal anti-GPIIb-IIIa antibodies, some GPIIIa (15% of normal amount) could be detected in the patient’s platelets, whereas GPIIb could not (<2% of normal amount). Nucleotide sequence analysis of platelet GPIIb mRNA-derived polymerase chain reaction (PCR) products revealed that patient’s GPIIb cDNA had a 75-bp deletion in the 3’ boundary of exon 17 resulting in an in-frame deletion of 25 amino acids. DNA analysis and family study revealed that the patient was a compound heterozygote of two GPIIb gene defects. One allele derived from her father was not expressed in platelets, and the other allele derived from her mother had a 9644C → T mutation which was located at the position -3 of the splice donor junction of exon 17 and resulted in a termination codon (TGA). Moreover, quantitative analysis demonstrated that the amount of the abnormal GPIIb transcript in the patient’s platelets was markedly reduced. Thus, the C → T mutation resulting in the abnormal splicing of GPIIb transcript and the reduction in its amount is responsible for Glanzmann’s thrombasthenia.

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Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽

10.33307/entomon.v44i3.466 ◽

2019 ◽

Vol 44 (3) ◽

pp. 213-218

Author(s):

Suresh Chand Kaushik ◽

Sukhvir Singh ◽

Purnima Srivastava ◽

R. Rajendran

Keyword(s):

Early Intervention ◽

Early Detection ◽

Aedes Aegypti ◽

Dengue Virus ◽

Infection Rate ◽

Transmission Season ◽

Aedes Mosquitoes ◽

Alternative Approach ◽

Human Sera ◽

Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

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IMMUNOLOGICAL REACTIVITY OF INSULIN IN HUMAN SERUM

Acta Endocrinologica ◽

10.1530/acta.0.0530673 ◽

1966 ◽

Vol 53 (4) ◽

pp. 673-680 ◽

Cited By ~ 5

Author(s):

Torsten Deckert ◽

Kai R. Jorgensen

Keyword(s):

Normal Subjects ◽

The Other ◽

Human Pancreas ◽

Porcine Pancreas ◽

Crystalline Insulin ◽

Endogenous Insulin ◽

Significant Difference ◽

Human Sera ◽

Normal Human ◽

The One

ABSTRACT The purpose of this study was to investigate whether a difference could be demonstrated between crystalline insulin extracted from normal human pancreas, and crystalline insulin extracted from bovine and porcine pancreas. Using Hales & Randle's (1963) immunoassay no immunological differences could be demonstrated between human and pig insulin. On the other hand, a significant difference was found, between pig and ox insulin. An attempt was also made to determine whether an immunological difference could be demonstrated between crystalline pig insulin and crystalline human insulin from non diabetic subjects on the one hand and endogenous, circulating insulin from normal subjects, obese subjects and diabetic subjects on the other. No such difference was found. From these experiments it is concluded that endogenous insulin in normal, obese and diabetic human sera is immunologically identical with human, crystalline insulin from non diabetic subjects and crystalline pig insulin.

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STUDIES ON SULFATION FACTOR (SF) ACTIVITY OF HUMAN SERUM

Acta Endocrinologica ◽

10.1530/acta.0.xxxv0381 ◽

1960 ◽

Vol XXXV (III) ◽

pp. 381-396 ◽

Cited By ~ 14

Author(s):

Sven Almqvist

Keyword(s):

Human Serum ◽

Normal Serum ◽

Treatment Level ◽

Response Curves ◽

Normal Children ◽

Significant Difference ◽

Human Sera ◽

Normal Humans ◽

Pre Treatment ◽

Dose Levels

ABSTRACT The sulfation factor (SF) activity of human sera has been estimated using a modification of the method of Daughaday et al. (1959). Each assay was statistically evaluated. The method had a mean precision of 0.14 and, used as an assay of GH of human serum, a sensitivity in three pituitary dwarfs of 0.1 to 0.6 μg of HGH/ml of serum. SF activity was found at all ages between 1 month and 75 years. There was a significantly lower mean SF activity below the age of half a year. Three cases of pituitary dwarfism had significantly low SF activities of sera. There was no significant difference between the SF activities of sera from untreated pituitary dwarfs and the sera from normal children below half a year of age. Dose-response curves with large volumes of sera from pituitary dwarfs and small volumes of sera from normal humans had the same slopes. Four mg of HGH prepared according to the method of Li & Papkoff (1956) resulted in a normal serum SF activity in each of the three dwarfs. A significant (P < 0.01) linear relationship was found between the concentration of SF activity of sera from these subjects and the logarithm of the dose of HGH given with dose levels of 1, 2 and 4 mg daily for three days. The decline of serum SF activity to the pre-treatment level following HGH in one dwarf suggested a half life not different from that indicated by others for growth hormone.

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Comparative Study of Widal and Dot Elisa in the Diagnosis of Typhoid Fever

International Journal of Scientific Research ◽

10.15373/22778179/apr2014/104 ◽

2012 ◽

Vol 3 (4) ◽

pp. 303-304

Author(s):

Cinthujah B Cinthujah B ◽

◽

Amudha VP Amudha VP ◽

Sucilathangam G Sucilathangam G

Keyword(s):

Comparative Study ◽

Typhoid Fever ◽

Dot Elisa

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