Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 213-218
Author(s):  
Suresh Chand Kaushik ◽  
Sukhvir Singh ◽  
Purnima Srivastava ◽  
R. Rajendran
Keyword(s):  
Early Intervention ◽  
Early Detection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Transmission Season ◽  
Aedes Mosquitoes ◽  
Alternative Approach ◽  
Human Sera ◽  
Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

Effects of Ascogregarina culicis (Eugregarinorida: Lecudinidae) on Mosquito Size and Dengue Virus Infection in Aedes aegypti (Diptera: Culicidae)

2020 ◽  
Author(s):  
Panpim Thongsripong ◽  
Dawn M Wesson
Keyword(s):  
Virus Infection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Wing Length ◽  
The Body ◽  
Global Public Health ◽  
Dengue Virus Infection ◽  
Significant Difference ◽  
The Impact

Abstract Dengue virus infection, transmitted via mosquito bites, poses a substantial risk to global public health. Studies suggest that the mosquito’s microbial community can profoundly influence vector-borne pathogen transmissions, including dengue virus. Ascogregarina culicis (Ross) of the phylum Apicomplexa is among the most common parasites of Aedes aegypti (Linnaeus), the principal vector of dengue. Despite a high prevalence worldwide, including in the areas where dengue is endemic, the impact of A. culicis on Ae. aegypti vector competence for dengue virus is unknown. This study aimed to investigate the effects of A. culicis infection on mosquito size and fitness, as measured by wing length, and the susceptibility to dengue virus infection in Ae. aegypti. Our results showed that there was no statistically significant difference in wing lengths between Ae. aegypti infected and not infected with A. culicis. Furthermore, A. culicis infection did not significantly affect dengue virus infection or disseminated infection rate. However, there was a significant association between shorter wings and higher dengue virus infection rate, whereby a 0.1-mm increase in wing length decreased the odds of the mosquito being infected by 32%. Thus, based on our result, A. culicis infection does not influence the body size and dengue virus infection in Ae. aegypti. This study helps to shed light on a common but neglected eukaryotic mosquito parasite.

scholarly journals The Effectiveness of Citrus Hystrix As Rapelant against Aedes Aegypti

2019 ◽  
Author(s):  
Anita Rosanty ◽  
Reni Yunus ◽  
Dian Yuniar SR
Keyword(s):  
Experimental Study ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Life Cycles ◽  
Case Study Design ◽  
Aedes Mosquitoes ◽  
Vector Mosquito

Indonesia is the country with the highest cases of dengue fever in Southeast Asia. The WHO estimates a 50–100 million dengue infections worldwide every year. Dengue Hemorrhagic Fever (DHF) is transmitted through the bite of female Aedes mosquitoes infected with the Dengue virus. Mosquitoes Aedes aegypti is the main vector of the dengue virus. At the time, there is no cure for dengue so that the control efforts are focused on breaking the chain of life cycles. One effort to prevent the transmission of the dengue virus is to avoid vector mosquito bites. Repellent can reduce exposure to the bite of mosquitoes infected with the dengue virus. The aim of this study was to determine the effectiveness of the extract of citrus hystrix leaf as a repellent against the Aedes aegypti mosquitoes. This research is a laboratory experimental study with a one-shot case study design. In this study, the extract of citrus hystrix solution was made at a concentration of 10%, 20%, and 30%, and then the extract solution was used as a stock to make a 100-gram base lotion that would be used as a repellent. Repellent effectiveness is seen from the percentage of repellent protection power. The percentage of repellant protection used with the basic ingredients of Citrus hystrix leaf extract at concentrations of 10%, 20%, and 30%, respectively, at 93.33% 94.67%, and 97.33%. The extract of citrus hystrix leaf was found to be effective as a repellent against Aedes aegypti mosquitoes.

scholarly journals Minimum infectious dose for chikungunya virus in Aedes aegypti and Ae. albopictus mosquitoes

2017 ◽  
Vol 41 ◽  
pp. 1
Author(s):  
Ann M Powers ◽  
Jeremy P Ledermann ◽  
Erin M Borland
Keyword(s):  
Aedes Aegypti ◽  
Incubation Period ◽  
Infection Rate ◽  
Chikungunya Virus ◽  
Entire Period ◽  
Infectious Dose ◽  
Extrinsic Incubation Period ◽  
Epidemiological Risk ◽  
Minimum Infection Rate

Understanding the ability of the chikungunya virus (CHIKV) to be transmitted by Aedes vectors in the Americas is critical for assessing epidemiological risk. One element that must be considered is the minimum infectious dose of virus that can lead to transmission following the extrinsic incubation period. This study aimed to determine the minimum infection rate for the two Aedes species studied. The results revealed that doses as low as 3.9 log10 plaque-forming units per mL (pfu/mL) of an Asian genotype CHIKV strain can lead to transmission by Ae. albopictus, and doses of at least 5.3 log10 pfu/mL from the same strain are needed for transmission from Ae. aegypti. These low infecting doses suggest that infected individuals may be infectious for almost the entire period of their viremia, and therefore, to prevent further cases, measures should be taken to prevent them from getting bitten by mosquitoes during this period.

scholarly journals Pengaruh Lama Penyimpanan Telur terhadap Transovarial Infection Rate Virus DEN-3 pada Nyamuk Aedes aegypti

2019 ◽  
pp. 143-150
Author(s):  
Tri Wahono ◽  
Sitti Rahmah Umniyati ◽  
Tri Baskoro Tunggul Satoto
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Virus Antigen ◽  
Laboratory Research ◽  
Adult Mosquito ◽  
Storage Duration ◽  
Epidemic Period ◽  
Egg Storage ◽  
Virus Survival

Transovarial transmission of dengue virus is important phenomenon causes dengue virus survival during inter-epidemic period. The purpose of this study was to analyze effect of egg storage duration on DEN-3 virus Transovarial Infection Rate (TIR) in Aedes aegypti mosquitoes. This was laboratory research with experimental design. Ae. aegypti was infected with the DEN-3 virus per-orally and allowed to undergo its gonotrophic cycle. Eggs stored at room temperature (25±3oC) and relative humidity (70±5%)  for 0, 1, 2 and 3 months. Hatched eggs then colonized until become adult mosquito. Sample was taken from colonies at 4, 8, 16 days old. Headsquash of mosquitoes was preparedwith Streptavidin Biotin Peroxidase Complex (SBPC) immunocytochemical method using DSSE 10 monoclonal antibody and detected the presence virus antigen. Transovarial infection rate was determined by counting percentage of positive samples. Data were analyzed by linear regression test. Result of this study showed that in 4, 8, 16 samples showed lowest TIR on 0 month storage (33,33%, 33,33%; 40,00%) and highest TIR observed on 2 month storage (76,67%; 66,67%, 76,67%). Statistical test showed significantly different result (p = 0,013) with α = 0,05 and R2 = 0,476. The duration of egg storage effect on DEN-3 virus TIR in Ae. aegypti mosquito with 47.6% contribution.

scholarly journals Infection rate of Aedes aegypti mosquitoes with dengue virus depends on the interaction between temperature and mosquito genotype

2017 ◽  
Vol 284 (1864) ◽  
pp. 20171506 ◽  
Author(s):  
A. Gloria-Soria ◽  
P. M. Armstrong ◽  
J. R. Powell ◽  
P. E. Turner
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Incubation Temperature ◽  
Disease Risk ◽  
Vector Competence ◽  
Dengue Infection ◽  
Denv Infection ◽  
Environment Interaction ◽  
Virus Genotype

Dengue fever is the most prevalent arthropod-transmitted viral disease worldwide, with endemic transmission restricted to tropical and subtropical regions of different temperature profiles. Temperature is epidemiologically relevant because it affects dengue infection rates in Aedes aegypti mosquitoes, the major vector of the dengue virus (DENV). Aedes aegypti populations are also known to vary in competence for different DENV genotypes. We assessed the effects of mosquito and virus genotype on DENV infection in the context of temperature by challenging Ae. aegypti from two locations in Vietnam, which differ in temperature regimes, with two isolates of DENV-2 collected from the same two localities, followed by incubation at 25, 27 or 32°C for 10 days. Genotyping of the mosquito populations and virus isolates confirmed that each group was genetically distinct. Extrinsic incubation temperature (EIT) and DENV-2 genotype had a direct effect on the infection rate, consistent with previous studies. However, our results show that the EIT impacts the infection rate differently in each mosquito population, indicating a genotype by environment interaction. These results suggest that the magnitude of DENV epidemics may not only depend on the virus and mosquito genotypes present, but also on how they interact with local temperature. This information should be considered when estimating vector competence of local and introduced mosquito populations during disease risk evaluation.

Difusi dan Pola Spasial Sebaran Penyakit Demam Berdarah Dengue (DBD) Di Kota Bandar Lampung

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Nurul Qamila ◽  
Agel Vidian Krama
Keyword(s):  
Public Health ◽  
Spatial Analysis ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Spatial Pattern ◽  
Dengue Hemorrhagic Fever ◽  
Health Problem ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Descriptive Method

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and is transmitted by the mosquito Aedes aegypti (Aa.aegypti). The population is still a public health problem that increases the number of sufferers and also widespread, with population and education. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the spatial pattern and the spread of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the descriptive method and spatial pattern of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. Keywords: DHF, Spatial Analysis

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