Effects of enhancing vitamin D status by 25-hydroxycholecalciferol supplementation, alone or in combination with calcium and phosphorus, on sternum mineralisation and breast meat quality in broilers

2017 ◽  
Vol 58 (4) ◽  
pp. 452-461 ◽  
Author(s):  
M. Bozkurt ◽  
S. Yalçin ◽  
B. Koçer ◽  
A. E. Tüzün ◽  
H. Akşit ◽  
...  
Keyword(s):  
Vitamin D ◽  
Meat Quality ◽  
Vitamin D Status ◽  
Breast Meat ◽  
Calcium And Phosphorus ◽  
25 Hydroxycholecalciferol

Intestinal CaBP: a new quantitive index of vitamin D deficiency in the rat

1975 ◽  
Vol 229 (3) ◽  
pp. 689-694 ◽  
Author(s):  
F Bronner ◽  
T Freund
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Calcium Intake ◽  
Calcium Binding ◽  
Plasma Calcium ◽  
Vitamin D Status ◽  
Calcium And Phosphorus ◽  
Dose Dependent Increase ◽  
Dose Dependent ◽  
25 Hydroxycholecalciferol

Rats raised from weaning on regiments adequate in calcium and phosphorus but deficient in vitamin D will have no detectable intestinal calcium-binding proteins (CaBP), whether or not they show other signs of vitamin D deficiency, such as hypocalcemia. When hypocalcemic, vitamin D-deficient animals were treated with 25-hydroxycholecalciferol, a vitamin D metabolite, they showed a dose-dependent increase in plasma calcium and CaBP; both responses can be described by a single linear relationship, which appears to apply whether the metabolite is 25-hydroxycholecalciferol or dihydrotachysterol. Since vitamin D status is only one determinant of plasma calcium, whereas CaBP (or its expression) appears to depend on vitamin D quantitatively, CaBP may be used as an index of vitamin D status, provided calcium intake is controlled.

scholarly journals Characteristics of the rat liver microsomal enzyme system converting cholecalciferol into 25-hydroxycholecalciferol. Evidence for the participation of cytochrome p-450

1979 ◽  
Vol 184 (3) ◽  
pp. 491-499 ◽  
Author(s):  
T C Madhok ◽  
H F DeLuca
Keyword(s):  
Vitamin D ◽  
White Light ◽  
Microsomal Fraction ◽  
Enzyme System ◽  
Initial Reaction ◽  
Microsomal Enzyme ◽  
Reaction Velocity ◽  
Calcium And Phosphorus ◽  
Cytochrome P 450 ◽  
25 Hydroxycholecalciferol

Properties of the rat hepatic cholecalciferol 25-hydroxylase have been studied. An assay system has been developed in which 25-hydroxycholecalciferol production is linear for at least 2h in both homogenates and microsomal fraction. Furthermore, the initial reaction velocity is linearly related to the amount of liver tissue or microsomal fraction. This enzyme system also metabolizes an analogue of cholecalciferol, namely dihydrotachysterol 3, into 25-hydroxydihydrotachysterol 3. The 25-hydroxylase is in the microsomal fraction and not in mitochondria. It has a Km of 44 nM for cholecalciferol and 360 nM for dihydrotachysterol 3. Its activity is not altered by dietary concentrations of calcium and phosphorus. Vitamin D-deficient rats have higher activities of the hepatic 25-hydroxylase than those receiving 25 ng of cholecalciferol daily. The 25-hydroxylase is inhibited by metyrapone. An atmosphere of CO/O2 (9:1, v/v) inhibits the reaction by 87%. This inhibition is partially reversed by white light. Additionally, cholecalciferol and 25-hydroxycholecalciferol competitively inhibit aminopyrine demethylase. These results support the idea that the cholecalciferol 25-hydroxylase is a cytochrome P-450-dependent mono-oxygenase.

Improved radioimmunoassay for vitamin D and its use in assessing vitamin D status.

1985 ◽  
Vol 31 (11) ◽  
pp. 1815-1819 ◽  
Author(s):  
B W Hollis ◽  
J L Napoli
Keyword(s):  
Vitamin D ◽  
High Titer ◽  
Rabbit Serum ◽  
Vitamin D Status ◽  
Ultraviolet Detection ◽  
Vitamin D Analog ◽  
Assay Tube ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic ◽  
25 Hydroxycholecalciferol

Abstract We describe a faster, more-sensitive radioimmunoassay for vitamin D in plasma. Antibodies were generated in rabbits immunized with a new vitamin D analog, the 23,24,25,26,27-pentanor-C(22)-carboxylic acid of vitamin D, coupled directly with bovine serum albumin. After several months, Rivanol-treated sera from the rabbits contained high-titer antibodies, as determined by their abilities to bind 25-hydroxy-[3H]cholecalciferol. The antibody, used at a 1:15 000 final dilution, cross reacted equally with all cholecalciferol and ergocalciferol metabolites tested except 1,25-dihydroxycalciferol metabolites and the parent calciferols. 25-Hydroxycalciferol and similar forms were efficiently extracted from plasma or serum with acetonitrile. We separated bound from free 25-hydroxy-[3H]cholecalciferol by using a second antibody: goat antiserum to rabbit serum. The detection limit of the assay was 3.0 pg of 25-hydroxycholecalciferol and its equivalents per tube; thus only 1 microL of plasma is needed per assay tube. Results compared well with those from a liquid-chromatographic procedure involving specific ultraviolet detection of 25-hydroxycalciferol in plasma.

Vitamin D homeostasis is compromised due to increased urinary excretion of the 25-hydroxycholecalciferol-vitamin D-binding protein complex in the Zucker diabetic fatty rat

2010 ◽  
Vol 299 (6) ◽  
pp. E959-E967 ◽  
Author(s):  
R. L. Anderson ◽  
S. B. Ternes ◽  
K. A. Strand ◽  
M. J. Rowling
Keyword(s):  
Vitamin D ◽  
Urinary Excretion ◽  
Binding Protein ◽  
Serum Vitamin ◽  
Vitamin D Status ◽  
Vitamin D Binding Protein ◽  
Renal Reabsorption ◽  
Vitamin D Levels ◽  
Zucker Diabetic Fatty Rats ◽  
25 Hydroxycholecalciferol

Altered serum concentrations of the major circulating form of vitamin D [25-hydroxycholecalciferol (25D3)] and its active hormone derivative [1,25-dihydroxycholecalciferol (1,25D3)] have been linked to non-insulin-dependent diabetes mellitus (NIDDM). However, a mechanistic basis for this occurrence has not been fully elucidated. Normally, renal reabsorption of vitamin D-binding protein-bound 25D3 absolutely requires receptor-mediated endocytosis via a receptor complex containing megalin, cubilin, and disabled-2 (Dab2), whereas an absence of megalin or its endocytic partners can lead to a marked urinary loss of 25D and severe vitamin D deficiency. Therefore, we hypothesized that reduced serum vitamin D status in NIDDM may be due to reduced expression of megalin and/or its endocytic partners and increased urinary excretion of protein-complexed 25D3. In the present study, we utilized Zucker diabetic fatty Rats (ZDF) to demonstrate that renal reuptake of the 25D3-DBP complex was compromised in ZDF animals, which was reflected by a reduction in expression of megalin and Dab2. Moreover, serum levels of both 25D3 and 1,25D3 were reduced, and urinary 25D3, 1,25D3, and DBP excretion were elevated in the ZDF animals compared with their lean controls regardless of vitamin D levels in the diet. Taken together, these are the first reports to our knowledge that associate compromised renal reabsorption of the 25D3-DBP complex with expression of megalin and its endocytic partners in NIDDM, which in turn can lead to compromised vitamin D status.

scholarly journals Rapid improvement in vitamin D status with dietary 25-hydroxycholecalciferol in vitamin D insufficient dogs

2021 ◽  
Vol 10 ◽  
Author(s):  
Rachel A. Kurzbard ◽  
Robert C. Backus ◽  
Shiguang Yu
Keyword(s):  
Vitamin D ◽  
Dietary Supplementation ◽  
Vitamin D Insufficiency ◽  
Vitamin D Status ◽  
Rapid Improvement ◽  
Initial Plasma ◽  
Body Weights ◽  
Healthy Dogs ◽  
Privately Owned ◽  
25 Hydroxycholecalciferol

Abstract Vitamin D insufficiency is associated with various disease processes. We determined whether consumption of a diet supplemented with HyD®, a 25-hydroxycholecalciferol (25(OH)D3) source, would safely increase plasma 25(OH)D3 concentrations in Golden Retrievers with low vitamin D status. We hypothesised that dietary supplementation with HyD® would rapidly increase and sustain plasma 25(OH)D3 levels in healthy Golden Retrievers with low vitamin D status compared with supplementation with vitamin D3. Of fifty-seven privately owned dogs recruited with written owner consent, eighteen dogs with low vitamin D status were identified and sorted between two groups to have similar initial plasma 25(OH)D3 concentrations, sex distributions, ages and body weights. Dogs of each group were fed a dry dog food supplemented with either 16 μg/kg of 25(OH)D3 as HyD® (n 10) or 81 μg/kg of cholecalciferol (D3) (n 8) for 4 months. Plasma 25(OH)D3 concentrations were determined monthly. A significant time effect (P < 0⋅001) and time by group interaction (P = 0⋅0045) were found for monthly determined plasma 25(OH)D3 concentrations. Dogs fed the HyD®-supplemented diet experienced a 40⋅5 % rise in plasma 25(OH)D3 values after 1 month (P < 0⋅001) and no change thereafter. Plasma 25(OH)D3 values of dogs supplemented with vitamin D3 did not increase (P > 0⋅05) and were less than values of dogs supplemented with HyD® (P = 0⋅044). With few exceptions, average haematologic, biochemical and urinalyses results remained within the reference range for both groups. Dietary supplementation with HyD® is sufficient to safely increase and sustain plasma 25(OH)D3 levels in healthy dogs.

scholarly journals Association between Fasting Glucose Concentration, Lipid Profile and 25(OH)D Status in Children Aged 9–11

Nutrients ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1359 ◽  
Author(s):  
Lukasz Szternel ◽  
Magdalena Krintus ◽  
Katarzyna Bergmann ◽  
Tadeusz Derezinski ◽  
Grazyna Sypniewska
Keyword(s):  
Vitamin D ◽  
Total Cholesterol ◽  
Impaired Fasting Glucose ◽  
Fasting Glucose ◽  
Density Lipoprotein ◽  
Cholesterol Concentration ◽  
Vitamin D Status ◽  
Total Cholesterol Concentration ◽  
The Relationship ◽  
25 Hydroxycholecalciferol

Background: The aim of this study was to assess the relationship between vitamin D status and the prevalence of dyslipidemia and impaired fasting glucose (IFG) in children. Methods and Summary: 284 children (150 boys and 134 girls) aged 9–11 were included in the study. Children with deficient 25(OH)D (25-hydroxycholecalciferol) levels ≤20 ng/mL (50 nmol/L) were characterized by a more frequent occurrence of impaired fasting glucose (IFG) (Odd ratios (OR) = 1.966, 95% confidence interval (CI): 1.055–3.663; p = 0.033) when compared to children with 25(OH)D >20 ng/mL. Serum 25(OH)D with concentration lower by 1 ng/mL (2.5 nmol/L) was linked to higher fasting glucose (by 0.25 mg/dL, 0.013 mmol/L; p = 0.017), higher total cholesterol (TC) by almost 1 mg/dL (0.96 mg/dL, 0.25 mmol/L; p = 0.006) and higher high-density lipoprotein cholesterol (HDL-C) (by 0.57 mg/dL, 0.015 mmol/L; p < 0.001). Conclusion: 25(OH)D deficiency may negatively affect fasting glucose and total cholesterol concentration in children aged 9–11. Vitamin D-deficient children are twice as likely to develop prediabetes as reflected by impaired fasting glucose when compared to those with a 25(OH)D level above 20 ng/mL (50 nmol/L).

Vitamin D-containing supplements for children between 1–3 years of age: Are they essential for bone health?

2015 ◽  
Vol 28 (1-2) ◽  
Author(s):  
Emel Torun ◽  
Feyza Ustabas Kahraman ◽  
Nurcan Keskin Osmanoğlu ◽  
Mehmet Kucukkoc ◽  
Tugrul Donmez ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Supplementation ◽  
Vitamin D Status ◽  
Vitamin D Levels ◽  
Different Seasons ◽  
Medical Reports ◽  
Calcium And Phosphorus ◽  
Low Levels ◽  
Phosphorus Levels ◽  
Group 1

Abstract: We aim to evaluate how vitamin D-containing supplements affect the vitamin D status of children 1–3 years old and to assess the variation of the vitamin D concentrations across different seasons.This retrospective study included a review of medical reports of 1035 patients (aged 1–3 years) who presented to our hospital between October 2011 and October 2013. The children were divided into 3 groups: Group 1: those supplemented with vitamin DGender distribution was not statistically different between the three groups (p=0.38). The children who had taken vitamin D-containing supplements had significantly higher vitamin D levels compared with the children who were given no vitamin D supplementation (p<0.001). Children with no vitamin D supplementation had significantly lower calcium and phosphorus levels compared with Groups 1 and 2. Vitamin D levels were affected by vitamin D supplementation (f=16.125, p<0.001) but not by season (f=0.699, p=0.650).The children aged 1–3 years who did not receive vitamin D-containing supplements had insufficient vitamin D and low levels of calcium and phosphorus. Vitamin D levels were affected by vitamin D supplementation but not by season.

Study of Vitamin D Status among Postmenopausal Women

2017 ◽  
Vol 5 (1) ◽  
pp. 28-34
Author(s):  
Nithya P Jayakumar ◽  
Bharathi Rao ◽  
Nikil Shetty
Keyword(s):  
Vitamin D ◽  
Dietary Intake ◽  
Vitamin D Deficiency ◽  
Postmenopausal Women ◽  
Serum Calcium ◽  
Sun Exposure ◽  
Serum Phosphorus ◽  
Vitamin D Status ◽  
Medical College ◽  
Calcium And Phosphorus

ABSTRACT Aims To evaluate vitamin D, serum calcium, and serum phosphorus levels in postmenopausal women attending gynecology outpatient department in Lady Goschen and Kasturba Medical College Attavar hospitals. Objective To study the prevalence of vitamin D deficiency among the studied group. To correlate vitamin D levels with diet and environmental factors. Materials and methods The study was conducted in 100 postmenopausal women attending Lady Goschen hospital and Kasturba Medical College Attavar hospital, Mangaluru. It was a hospital-based, prospective cross-sectional study conducted over the period between August 2014 and June 2016. Levels of vitamin D and serum calcium and phosphorus were assessed in them. Results A total of 11% were found to have vitamin D deficiency, 38 and 48% had insufficient levels and sufficient levels of vitamin D respectively, 53% had normal levels of serum calcium, and 47% had inadequate levels. About 70% were found to have normal levels of serum phosphorus and 24% had inadequate levels. Dietary intake was found to be inadequate in 61% of the studied population. Serum calcium and phosphorus deficiency had significant corelation with vitamin D deficiency. Also significant corelation was noted with sun exposure and dietary intake in relation to vitamin D. No corelation was established between hypertension, diabetes and vitamin D. Conclusion This study helped us to assess basal levels of vitamin D, serum calcium, and serum phosphorus among postmenopausal women, giving us an idea regarding prevalence of vitamin D deficiency among South Indian postmenopausal women. We should consider counseling all postmenopausal patients regarding the benefit of vitamin D supplementation, adequate dietary intake, and sun exposure. How to cite this article Jayakumar NP, Rao B, Shetty N. Study of Vitamin D Status among Postmenopausal Women. J South Asian Feder Menopause Soc 2017;5(1):28-34.

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