Survey and etiology of bacterial brood disease infecting Indian honey bees (Apis cerana indica F.) in Southern Kerala

2019 ◽  
Vol 59 (4) ◽  
pp. 519-527
Author(s):  
Jyothis P. Joseph ◽  
Amritha V. S.
Keyword(s):  
Honey Bees ◽  
Apis Cerana ◽  
Apis Cerana Indica

scholarly journals Hypopharyngeal Gland Activity in Task-Specific Workers Under Brood and Broodless Conditions in Apis Cerana Indica (Fab.)

2014 ◽  
Vol 58 (2) ◽  
pp. 59-70 ◽  
Author(s):  
Seydur Rahman ◽  
Ibamelaker Thangkhiew ◽  
Sudhanya R. Hajong
Keyword(s):  
Electron Microscopy ◽  
Protein Synthesis ◽  
Honey Bees ◽  
Protein Concentration ◽  
Apis Cerana ◽  
Larval Rearing ◽  
Hypopharyngeal Gland ◽  
Head Weight ◽  
Apis Cerana Indica ◽  
Scanning Electron

Abstract The hypopharyngeal gland (HPG) is the principal organ of protein synthesis in honey bees. It is involved in larval rearing. We examined the fresh head weight, HPG acini diameter, and HPG protein content in worker bees engaged in different tasks and under brood and broodless conditions. Scanning electron microscopy revealed that the HPG acini diameter of worker bees was related to their task. The highest HPG volume was found in nurse bees, and the volume regressed when the task changed from guarding to foraging. The fresh head weight was positively correlated with HPG acini diameter. Although, there was no positive correlation between HPG acini diameter and protein concentration, the glandular protein concentration increased progressively in nurse bees and declined in guard and forager bees. Histochemistry revealed similar results. Despite displaying significantly larger glands, guard bee protein secretion was similar to that of the foragers. Brooding had a significant effect on HPG activity. Only worker bees from the colony with an intact brood showed elevated rates of protein synthesis; thus, it is possible that a signal was emitted by the brood, which stimulated protein synthesis in the HPG. However, the size of the HPG was similar in both brood and broodless conditions.

scholarly journals Antiviral Activities of a Medicinal Plant Extract Against Sacbrood Virus in Honeybees

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Liping Sun ◽  
Xueqi Zhang ◽  
Shufa Xu ◽  
Chunsheng Hou ◽  
Jin Xu ◽  
...  
Keyword(s):  
Honey Bees ◽  
Apis Cerana ◽  
Larval Growth ◽  
Antiviral Agent ◽  
Antiviral Effect ◽  
Absolute Quantification ◽  
Chinese Medicinal Herb ◽  
Sacbrood Virus ◽  
Antiviral Activities ◽  
Potential Strategy

Abstract Background Sacbrood is an infectious disease of the honey bee caused by Scbrood virus (SBV) which belongs to the family Iflaviridae and is especially lethal for Asian honeybee Apis cerana. Chinese Sacbrood virus (CSBV) is a geographic strain of SBV. Currently, there is a lack of an effective antiviral agent for controlling CSBV infection in honey bees. Methods Here, we explored the antiviral effect of a Chinese medicinal herb Radix isatidis on CSBV infection in A. cerana by inoculating the 3rd instar larvae with purified CSBV and treating the infected bee larvae with R. isatidis extract at the same time. The growth, development, and survival of larvae between the control and treatment groups were compared. The CSBV copy number at the 4th instar, 5th instar, and 6th instar larvae was measured by the absolute quantification PCR method. Results Bioassays revealed that R. isatidis extract significantly inhibited the replication of CSBV, mitigated the impacts of CSBV on larval growth and development, reduced the mortality of CSBV-infected A. cerana larvae, and modulated the expression of immune transcripts in infected bees. Conclusion Although the mechanism underlying the inhibition of CSBV replication by the medicine plant will require further investigation, this study demonstrated the antiviral activity of R. isatidis extract and provides a potential strategy for controlling SBV infection in honey bees.

Behavioral responses of honey bees, Apis cerana and Apis mellifera, to Vespa mandarinia marking and alarm pheromones

2018 ◽  
Vol 58 (1) ◽  
pp. 141-148 ◽  
Author(s):  
Beverly McClenaghan ◽  
Marcel Schlaf ◽  
Megan Geddes ◽  
Joshua Mazza ◽  
Grace Pitman ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Apis Cerana ◽  
Behavioral Responses ◽  
Alarm Pheromones

scholarly journals Complete genome sequence of sacbrood virus isolated from Asiatic honey bee Apis cerana indica in India

VirusDisease ◽  
2018 ◽  
Vol 29 (4) ◽  
pp. 453-460 ◽  
Author(s):  
R. Aruna ◽  
M. R. Srinivasan ◽  
V. Balasubramanian ◽  
R. Selvarajan
Keyword(s):  
Honey Bee ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Apis Cerana ◽  
Sacbrood Virus ◽  
Apis Cerana Indica

Absence of Wolbachia in the workers of Asian honeybee, Apis cerana indica

2012 ◽  
Vol 5 (1) ◽  
pp. 19-24
Author(s):  
Mahesh Pattabhiramaiah ◽  
Dorothea Brueckner ◽  
MS Reddy
Keyword(s):  
Apis Cerana ◽  
Life Sciences ◽  
Present Communication ◽  
Chain Reaction ◽  
Reproductive Incompatibility ◽  
Apis Cerana Indica ◽  
Wolbachia Endosymbiont ◽  
Polymerase Chain ◽  
Asian Honeybee ◽  
Different Parts

Wolbachia is a group of cytoplasmically inherited bacteria that can cause reproductive alterations in arthropods including parthenogenesis, reproductive incompatibility and feminization of genetic males. Wolbachia are found in a well studied group of insects, but there is a lack of data on their distribution. Workers of the honeybee sub species Apis cerana indica, collected from different parts of Karnataka, India were screened by PCR for Wolbachia, because this endosymbiont has been implicated in causing thelytoky in other Hymenoptera. In the present communication, we report the absence of Wolbachia endosymbiont in the workers of honeybee collected from different geographic locations of Karnataka using Wolbachia specific 16S rDNA polymerase chain reaction enzymes.DOI: http://dx.doi.org/10.3126/ijls.v5i1.5230 International Journal of Life Sciences Vol.5(1) 2011 19-24

scholarly journals Discovery of Aphid Lethal Paralysis Virus in Vespa velutina and Apis cerana in China

Insects ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 157 ◽  
Author(s):  
Dahe Yang ◽  
Hongxia Zhao ◽  
Junming Shi ◽  
Xiang Xu ◽  
Yanyan Wu ◽  
...  
Keyword(s):  
Honey Bees ◽  
Apis Cerana ◽  
Terrestrial Ecosystems ◽  
Viral Reservoir ◽  
Molecular Evidence ◽  
Deformed Wing Virus ◽  
Vespa Velutina ◽  
Pollinator Abundance ◽  
The Usa ◽  
Paralysis Virus

Honey bees are essential to the functioning of terrestrial ecosystems. However, despite no single factor being blamed for losses of honey bee colonies in Europe and the USA, viruses have been considered as a major driver. Moreover, a virus vector can enhance the titer and virulence of virus such as Varroa destructor can change the virulence of the deformed wing virus. Here, we report molecular evidence for aphid lethal paralysis virus (ALPV) infecting Vespa velutina, which is an important predator of honey bees, especially of Apis cerana. Viral replication and phylogenetic analysis indicated that ALPV can not only replicate in V. velutina and A. cerana, but ALPV from A. cerana (ALPV-Ac) was also significantly associated with that of V. velutina (ALPV-Vv), though distinct from those of Apis mellifera (ALPV-Am). The host state posterior probability displayed that V. velutina is the main viral reservoir between V. velutina and A. cerana. Our results show ALPV had expanded host diversity resulting in potential impacts on the health of pollinators, even on the pollination ecosystem. We suggest further studies should investigate potential risks and impacts on pollinator populations of hornets. These results should have an impact conservation efforts focused on sustaining native pollinator abundance and diversity, and therefore, the crucial ecosystem services that they provide.

scholarly journals SEM Investigation of Pollen Taxa in Honeys from Autochtone Apis cerana in Godavari, Lalitpur District, Nepal

2015 ◽  
Vol 26 ◽  
pp. 29-67 ◽  
Author(s):  
Khum Narayan Paudayal ◽  
Ishan Gautam
Keyword(s):  
Honey Bees ◽  
Apis Cerana ◽  
Pollen Grains ◽  
Species Level ◽  
Palynological Assemblage ◽  
Wide Range ◽  
Multifloral Honey ◽  
Plant Sources ◽  
Sem Investigation ◽  
Scanning Electron

Pollen analysis of 8 multifloral honey samples collected from 4 locations of Godavari, Lalitpur district, Nepal was performed using Scanning Electron Microscope (SEM). In this investigation, a wide range of foraging plant sources for Apis cerana honey bees was identified which demonstrates the adequate potential for expanding and sustaining beekeeping in this area. The palynological assemblage of a total of 44 species of pollen flora representing 28 families was identified to the generic and some up to species level. Some of the pollen grains identified to only families, belong to Acanthaceae, Apiaceae, Araliaceae, Chenopodiaceae, Compositae, Lamiaceae, Loranthaceae, Meliaceae, Poaceae, Rosaceae, Rutaceae and Pteridaceae. The pollen assemblages in honeys were mostly belonging to angiosperms while the gymnosperm pollen was completely absent. One pteridophyte spore belonging to family Pteridaceae recovered. In this paper the morphology of the pollen grains based on SEM observation are described and the importance of the systematic documentation of various bee flora are discussed.J. Nat. Hist. Mus. Vol. 26, 2012: 29-67

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