Absence of Wolbachia in the workers of Asian honeybee, Apis cerana indica

2012 ◽  
Vol 5 (1) ◽  
pp. 19-24
Author(s):  
Mahesh Pattabhiramaiah ◽  
Dorothea Brueckner ◽  
MS Reddy
Keyword(s):  
Apis Cerana ◽  
Life Sciences ◽  
Present Communication ◽  
Chain Reaction ◽  
Reproductive Incompatibility ◽  
Apis Cerana Indica ◽  
Wolbachia Endosymbiont ◽  
Polymerase Chain ◽  
Asian Honeybee ◽  
Different Parts

Wolbachia is a group of cytoplasmically inherited bacteria that can cause reproductive alterations in arthropods including parthenogenesis, reproductive incompatibility and feminization of genetic males. Wolbachia are found in a well studied group of insects, but there is a lack of data on their distribution. Workers of the honeybee sub species Apis cerana indica, collected from different parts of Karnataka, India were screened by PCR for Wolbachia, because this endosymbiont has been implicated in causing thelytoky in other Hymenoptera. In the present communication, we report the absence of Wolbachia endosymbiont in the workers of honeybee collected from different geographic locations of Karnataka using Wolbachia specific 16S rDNA polymerase chain reaction enzymes.DOI: http://dx.doi.org/10.3126/ijls.v5i1.5230 International Journal of Life Sciences Vol.5(1) 2011 19-24

scholarly journals KERAGAMAN GENETIK, KEBUGARAN DAN INKOMPATIBILITAS REPRODUKSI Hemiptarsenus varicornis Girault (Hymenoptera: Eulophidae), PARASITOID LARVA Liriomyza huidobrensis (Diptera: Agromyzidae)

2011 ◽  
Vol 11 (1) ◽  
pp. 1-10
Author(s):  
Reflinaldon Reflinaldon ◽  
Damayanti Buchori ◽  
Dwinardi Aprianto
Keyword(s):  
Genetic Variation ◽  
Dna Polymerase ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Coefficient ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Reproductive Incompatibility ◽  
Liriomyza Huidobrensis ◽  
Geographic Populations ◽  
Polymerase Chain

Several experiments have been conducted to study genetic variation, fitness and reproductive incompatibility of H. varicornis from different geographic populations.  Genetic variation from Pandai Sikek (PS), Alahan Panjang (AP) and Kayu Aro (KA) was analyzed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique and the similarity of genetics measured using NTSys program. The fitness of female wasps such as longevity, fecundity and preoviposition was observed and then compared among those populations.  Incompatibility in reproduction was determined by accounting of reproductive compatibility (RC) index in crossing of intra and interpopulation both of PS and AP.  The results showed high genetic variation of H. varicornis among population from Alahan Panjang, Pandai Sikek and Kayu Aro with similarity coefficient of 30 to 70%.  The best fitness showed the female wasps from Kayu Aro that was significantly different (P= 0.00) in longevity (24.60 ± 6.4 days), fecundity (63.6 ± 28.6 eggs) and parasitization (53.60%) but not significantly different (P=0.07) in number of the first day eggs (1.1 ± 1.4 eggs). Crossing of AP and PS indicated incompatibility in reproduction among the population.

scholarly journals Presence of Pseudomonas syringae pv actinidiae the causal agent of bacterial canker of kiwifruit on symptomatic and asymptomatic tissues of kiwifruit

2011 ◽  
Vol 64 ◽  
pp. 241-245 ◽  
Author(s):  
J.L. Vanneste ◽  
J. Yu ◽  
D.A. Cornish ◽  
S. Max ◽  
G. Clark
Keyword(s):  
Pseudomonas Syringae ◽  
Vascular System ◽  
Causal Agent ◽  
Bacterial Canker ◽  
Effective Strategy ◽  
Bacterial Isolation ◽  
Chain Reaction ◽  
Leaf Spots ◽  
Polymerase Chain ◽  
Different Parts

Pseudomonas syringae pv actinidiae (Psa) the causal agent of bacterial canker of kiwifruit has recently been found in New Zealand This pathogen has been the object of few studies and little is known about its epidemiology Yet the development of an effective strategy of control requires an understanding of the life cycle of the pathogen in particular determining the location of the bacteria in tissues In this study the presence of Psa on flowers symptomatic and asymptomatic leaves and different parts of canes showing symptoms was determined by polymerase chain reaction (PCR) or by direct bacterial isolation Psa was found associated with angular necrotic leaf spots with white exudate and in canes In canes Psa seemed to be either in the parenchyma leading to production of exudate or in the vascular system leading to wilting Psa was also found on asymptomatic tissues where it was probably surviving as an epiphyte

scholarly journals Pathotypes of wheat leaf rust (Puccinia triticina Eriks.) and resistance of registered cultivars in the Czech Republic in 2012–2015

2017 ◽  
Vol 53 (No. 3) ◽  
pp. 122-126 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš ◽  
T. Sumíková
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Near Isogenic Lines ◽  
Wheat Leaf Rust ◽  
Chain Reaction ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Polymerase Chain ◽  
Different Parts

In 2012–2015 the virulence of the wheat leaf rust (Puccinia triticina Eriks.) population was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr13, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust were obtained from different parts of the Czech Republic. A total of 163 wheat leaf rust isolates were analysed. No virulence for the resistance gene Lr9 was found. Virulence for Lr19 was found only in one isolate in 2015. A lower frequency of virulence to Lr24, Lr2a, 2b, 2c and Lr28 was also observed. The presence of Lr10, Lr24, Lr26, Lr28 and Lr37 in registered cultivars was detected by polymerase chain reaction (PCR) molecular markers.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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