To be or not to be a species: use of reproductive isolation experiments and genetic analysis to clarify the taxonomic status of twoBusseola(Lepidoptera: Noctuidae) species in Kenya

2013 ◽  
Vol 49 (3) ◽  
pp. 345-354 ◽  
Author(s):  
Anne-Emmanuelle Félix ◽  
Paul-André Calatayud ◽  
Bruno Le Ru ◽  
Claire Capdevielle-Dulac ◽  
George Ong’amo ◽  
...  
Zootaxa ◽  
2017 ◽  
Vol 4329 (4) ◽  
pp. 392 ◽  
Author(s):  
TIN-YAM CHAN ◽  
APPUKUTTANNAIR BIJU KUMAR ◽  
CHIEN-HUI YANG

The availability of abundant fresh material of Aristeus alcocki Ramadan, 1938 from India allowed the evaluation of the variation in the numbers of photophores on the pereiopods in this species, as well as other diagnostic characters for species discrimination. Although the pereiopodal photophore counts in A. alcocki largely overlap with those of A. semidentatus Bate, 1888, it is found that A. alcocki is unique in the Indo-West Pacific species of the genus by the lower end of the cervical carina considerably farther away from the branchiostegal carina. Molecular genetic analysis confirmed the distinct taxonomic status of the six currently known species in this genus from the Indo-West Pacific and a revised key is provided for distinguishing them. 


Biologia ◽  
2012 ◽  
Vol 67 (6) ◽  
Author(s):  
Petr Benda ◽  
Peter Vallo ◽  
Pavel Hulva ◽  
Ivan Horáček

AbstractTwo metrically defined subspecies have traditionally been recognised within the Palaearctic distribution range of the Egyptian fruit bat Rousettus aegyptiacus; the larger R. a. aegyptiacus in Egypt and the northern part of the Middle East and the smaller R. a. arabicus in the southern Middle East. An extensive material of R. aegyptiacus from all parts of this area, i.e. the Levant (incl. Turkey and Cyprus), Egypt (incl. Sinai), northern Sudan, Yemen, Oman, Iran, and Pakistan, as well as comparative samples from its sub-Saharan range, were tested using both morphological and genetic approaches in order to revise the species’ taxonomic status. The results indicated two possible processes, depending on the method used. Genetic analysis of the mitochondrial genome (nd1 and cytb) indicated low variation (< 2.0% of genetic distance) and lack of geographical structure while morphometric analysis indicated significant metric differences. Two basic size morphotypes were found within the Palaearctic range, with a rather mosaic-like geographical distribution and a lack of clear size distinction between the two categories, though intermediate types were detected. Thus, we suggest that all Palaearctic populations of R. aegyptiacus represent one form, the nominotypical subspecies, which is uniform in genetic traits but plastic in metric traits.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maciej Karpowicz ◽  
Magdalena Świsłocka ◽  
Joanna Moroz ◽  
Łukasz Sługocki

AbstractThe taxonomic status of the genus Bythotrephes Leydig (Crustacea: Cladocera) has been debated since the second half of the XIX century. The most widespread view of recent decades has been that Bythotrephes is a monotypic genus, which was support by preliminary molecular data. However, the recent detailed morphological revision of this genus clearly distinguishes at least seven species. Therefore, we performed a multi-lake survey in Central Europe to give new insight into the taxonomic status of Bythotrephes by combining genetic analysis with traditional morphology-based taxonomy. Based on the morphology we identified two species in Central Europe, B. brevimanus and B. lilljeborgi, as well as hybrid forms. For the genetic analysis, we used newly obtained 113 sequences of mtDNA COI gene of the 535-bp length Bythotrephes from Central Europe and sequences downloaded from GenBank. There were no significant differences between all analyzed sequences, which supports the hypothesis that Bythotrephes is a monotypic genus, with only one highly polymorphic species. On the other hand, the results of our work could point out that the COI gene is insufficient to evaluate the taxonomic status of Bythotrephes. Nonetheless, we have identified 29 new haplotypes of mtDNA COI, and one which was the same as the haplotype found in North America and Finland. Furthermore, this haplotype was the source variant from which most other haplotypes were derived.


1985 ◽  
Vol 75 (2) ◽  
pp. 185-197 ◽  
Author(s):  
Jeffrey L. K. Hii

AbstractExamination of the polytene chromosomes and cross-breeding data confirmed the specific status of Anopheles dirusPeyton & Harrison within the complex of A. balabacensis Baisas. The data also strongly suggested that A. dirus itself is a complex of at least two species, one of which, a hitherto unrecognized species, provisionally designated A. dirus species B, previously known as the Perlis form, occurs in northern Peninsular Malaysia. A distinct population from Thailand was designated A. dirus species A. The mosquitoes studied also include material from natural populations in Sabah, here provisionally designated A. balabacensis s.s., and may represent the first genetic analysis of this species. The Sabah material is distinct from either of the A. dirus forms. Hybrid males from crosses of A. balabacensis × A. dirus species A and of A. dirus species B females × A. dirus species A males were sterile. Chromosome studies also showed that reproductive isolation was accompanied by genetic changes in both the autosomesand X chromosome in the hybrid larvae.


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