Protective effects of calcium from nonfat dried milk against colon carcinogenesis in rats

The mushroom Agaricus blazei ( Ab) has been widely used in folk medicine to treat various diseases including cancer. No information is available on its possible protective effects on the development of colon cancer. The potential blocking effect of Ab intake on the initiation stage of colon carcinogenesis was investigated in a short-term (4-week) bioassay using aberrant crypt foci (ACF) as biomarker. Male Wistar rats were given four subcutaneous injections of the carcinogen 1,2-dimethylhydrazine (DMH, 40 mg/kg bw, twice a week), during 2 weeks to induce ACF. The diet containing Ab at 5% was given 2 weeks before and during carcinogen treatment to investigate the potential beneficial effects of this edible mushroom on DMH-induced ACF. All groups were killed at the end of the fourth week. The colons were analyzed for ACF formation in 1% methylene blue whole-mount preparations and for cell proliferation in histological sections immunohistochemically stained for the proliferating cell nuclear antigen (PCNA). All DMH-treated rats developed ACF mainly in the middle and distal colon. Agaricus blazei intake at 5% did not alter the number of ACF induced by DMH or the PCNA indices in the colonic mucosa. Thus, the results of the present study did not confirm a chemopreventive activity of Ab on the initiation stage of rat colon carcinogenesis.

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Protective effects of sodium selenite and selenium nanoparticles against experimental colon carcinogenesis in mice

Journal of Preventive Veterinary Medicine ◽

10.13041/jpvm.2016.40.3.101 ◽

2016 ◽

Vol 40 (3) ◽

pp. 101-108 ◽

Cited By ~ 3

Author(s):

Do-Youn Jang ◽

◽

Sung-June Kim ◽

Jae-Hwang Jeong ◽

Sang Yoon Nam ◽

...

Keyword(s):

Sodium Selenite ◽

Colon Carcinogenesis ◽

Selenium Nanoparticles ◽

Protective Effects

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Effectiveness of Bioactive Food Components in Experimental Colon Carcinogenesis

Acta Veterinaria Brno ◽

10.2754/avb200978040661 ◽

2009 ◽

Vol 78 (4) ◽

pp. 661-666 ◽

Cited By ~ 3

Author(s):

Emília Hijová ◽

Anna Chmelárová ◽

Alojz Bomba

Keyword(s):

Bile Acid ◽

High Fat Diet ◽

Colon Carcinogenesis ◽

Control Group ◽

Serum Bile Acid ◽

Protective Effects ◽

High Fat ◽

Bile Acid Concentration ◽

Food Components ◽

Bioactive Food Components

The aim of the present study was the evaluation of possible protective effects of selected bioactive food components in experimental N,N-dimethylhydrazine (DMH)-induced colon carcinogenesis. Wistar albino rats (n = 92) were fed a high fat diet or conventional laboratory diet. Two weeks after the beginning of the trial, DMH injections were given to six groups of rats at the dose of 20 mg/kg b.w. twice weekly. The activity of bacterial enzymes in faeces and serum bile acid concentrations were determined. High fat diet, DMH injections, and their combination significantly increased the activies of β-galactosidase, β-glucuronidase, and α-glucosidase (p < 0.001) compared to the control group of rats. Treatment with the prebiotic inulin, Hyppocastani extractum siccum and Lini oleum virginale significantly decreased the activity of β-galactosidase, β-glucuronidase, and α-glucosidase (p < 0.001), as well as the bile acid concentration compared to the group at the highest risk. The protective effects of selected bioactive food components in experimentally induced colon carcinogenesis allow for their possible use in cancer prevention or treatment.

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