Protective effects of sodium selenite and selenium nanoparticles against experimental colon carcinogenesis in mice

2016 ◽  
Vol 40 (3) ◽  
pp. 101-108 ◽  
Author(s):  
Do-Youn Jang ◽  
◽  
Sung-June Kim ◽  
Jae-Hwang Jeong ◽  
Sang Yoon Nam ◽  
...  
Keyword(s):  
Sodium Selenite ◽  
Colon Carcinogenesis ◽  
Selenium Nanoparticles ◽  
Protective Effects

Lack of chemoprevention of dietary Agaricus blazei against rat colonic aberrant crypt foci

2008 ◽  
Vol 27 (6) ◽  
pp. 505-511 ◽  
Author(s):  
L Ziliotto ◽  
LF Barbisan ◽  
MAM Rodrigues
Keyword(s):  
Folk Medicine ◽  
Colon Carcinogenesis ◽  
Distal Colon ◽  
Edible Mushroom ◽  
Aberrant Crypt Foci ◽  
Nuclear Antigen ◽  
Rat Colon ◽  
Protective Effects ◽  
Agaricus Blazei ◽  
Initiation Stage

The mushroom Agaricus blazei ( Ab) has been widely used in folk medicine to treat various diseases including cancer. No information is available on its possible protective effects on the development of colon cancer. The potential blocking effect of Ab intake on the initiation stage of colon carcinogenesis was investigated in a short-term (4-week) bioassay using aberrant crypt foci (ACF) as biomarker. Male Wistar rats were given four subcutaneous injections of the carcinogen 1,2-dimethylhydrazine (DMH, 40 mg/kg bw, twice a week), during 2 weeks to induce ACF. The diet containing Ab at 5% was given 2 weeks before and during carcinogen treatment to investigate the potential beneficial effects of this edible mushroom on DMH-induced ACF. All groups were killed at the end of the fourth week. The colons were analyzed for ACF formation in 1% methylene blue whole-mount preparations and for cell proliferation in histological sections immunohistochemically stained for the proliferating cell nuclear antigen (PCNA). All DMH-treated rats developed ACF mainly in the middle and distal colon. Agaricus blazei intake at 5% did not alter the number of ACF induced by DMH or the PCNA indices in the colonic mucosa. Thus, the results of the present study did not confirm a chemopreventive activity of Ab on the initiation stage of rat colon carcinogenesis.

scholarly journals Effectiveness of Bioactive Food Components in Experimental Colon Carcinogenesis

2009 ◽  
Vol 78 (4) ◽  
pp. 661-666 ◽  
Author(s):  
Emília Hijová ◽  
Anna Chmelárová ◽  
Alojz Bomba
Keyword(s):  
Bile Acid ◽  
High Fat Diet ◽  
Colon Carcinogenesis ◽  
Control Group ◽  
Serum Bile Acid ◽  
Protective Effects ◽  
High Fat ◽  
Bile Acid Concentration ◽  
Food Components ◽  
Bioactive Food Components

The aim of the present study was the evaluation of possible protective effects of selected bioactive food components in experimental N,N-dimethylhydrazine (DMH)-induced colon carcinogenesis. Wistar albino rats (n = 92) were fed a high fat diet or conventional laboratory diet. Two weeks after the beginning of the trial, DMH injections were given to six groups of rats at the dose of 20 mg/kg b.w. twice weekly. The activity of bacterial enzymes in faeces and serum bile acid concentrations were determined. High fat diet, DMH injections, and their combination significantly increased the activies of β-galactosidase, β-glucuronidase, and α-glucosidase (p < 0.001) compared to the control group of rats. Treatment with the prebiotic inulin, Hyppocastani extractum siccum and Lini oleum virginale significantly decreased the activity of β-galactosidase, β-glucuronidase, and α-glucosidase (p < 0.001), as well as the bile acid concentration compared to the group at the highest risk. The protective effects of selected bioactive food components in experimentally induced colon carcinogenesis allow for their possible use in cancer prevention or treatment.

scholarly journals Radiolytic Synthesis and Characterization of Selenium Nanoparticles: Comparative Biosafety Evaluation with Selenite and Ionizing Radiation

2021 ◽  
Author(s):  
Alline Gomes Pereira ◽  
Luanai Graziele Luquini Gerolis ◽  
Letícia Satler Gonçalves ◽  
Luciana Mara Costa Moreira ◽  
Pedro Lana Gastelois ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ionizing Radiation ◽  
Radiation Exposure ◽  
Sodium Selenite ◽  
Structural Parameters ◽  
Reactive Oxygen Species Generation ◽  
Protein Carbonylation ◽  
Selenium Nanoparticles ◽  
Spherical Particles ◽  
Water Radiolysis

Abstract Selenium nanoparticles (SeNPs) were synthesized by irradiating a solution containing sodium selenite (Se+4) as the precursor in YPG liquid medium with gamma-rays. Spherical particles were formed after reactions of sodium selenite with hydrated electrons (eaq-) and hydrogen radicals (H•) produced following water radiolysis. No hazardous reducing agents were employed. The obtained nanoparticles were morphologically characterized, and their physicochemical and structural parameters were analyzed. SeNPs characterization showed all selenium in the Se0 state. We incubated Saccharomyces cerevisiae cells with the SeNPs for 24 h and then challenged the cells with ionizing radiation. After radiation exposure, cells were assessed for cell viability, lipid peroxidation, protein carbonylation, free radical generation, and total sulfhydryl content. The synthesized SeNPs were considered safe and less toxic at the concentration employed than the same selenite concentration. Except for the protein carbonylation results, there were no other significant modifications in viability or the oxidative stress parameters in SeNP-treated cells. It was concluded that 1 mM of the synthesized SeNPs does not trigger oxidative stress. Furthermore, we verified that SeNPs attenuate the reactive oxygen species generation after in vitro ionizing radiation exposure. These observations open up tremendous possibilities for radiosensitizer development.

scholarly journals A Study on Myogenesis by Regulation of Reactive Oxygen Species and Cytotoxic Activity by Selenium Nanoparticles

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1727
Author(s):  
Sang-Cheol Lee ◽  
Na-Hyun Lee ◽  
Kapil D. Patel ◽  
Soo-Kyung Jun ◽  
Jeong-Hui Park ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Skeletal Muscle ◽  
Contractile Activity ◽  
Reactive Oxygen ◽  
Selenium Nanoparticles ◽  
C2c12 Cells ◽  
Myoblast Differentiation ◽  
Oxygen Species ◽  
Protective Effects ◽  
Intracellular Ros

Reactive oxygen species (ROS) are continuously produced by skeletal muscle during contractile activity and even at rest. However, the ROS generated from excessive exercise or traumatic damage may produce more ROS than can be neutralized by an antioxidant capacity, which can be harmful to muscle function. In particular, selenium is a known antioxidant that regulates physiological functions such as cell differentiation and anti-inflammatory function. In this study, we developed nano-sized antioxidative biomaterials using selenium to investigate the protective and differentiation effects against C2C12 myoblasts in an H2O2-induced oxidative stress environment. The selenium nanoparticles (SeNPs) were produced with a size of 35.6 ± 4.3 nm and showed antioxidant effects according to the 3,3′,5,5′-tetramethylbenzidine assay. Then, SeNPs were treated to C2C12 cells with or without H2O2. Our results showed that SeNPs reduced C2C12 apoptosis and intracellular ROS levels. Additionally, SeNPs effectively up-regulated in the presence of H2O2, MyoD, MyoG, α-actinin, and myosin heavy chain, which are well known to increase during myoblast differentiation as assayed by qRT-PCR, immunocytochemistry-staining, western blotting. These results demonstrate that SeNPs can accelerate differentiation with its protective effects from the ROS environment and can be applied to the treatment of skeletal muscle in a cellular redox environment.

Protective effects of selenium nanoparticle-enriched Lactococcus lactis NZ9000 against enterotoxigenic Escherichia coli K88-induced intestinal barrier damage in mice

2021 ◽  
Author(s):  
Yue Chen ◽  
Lei Qiao ◽  
Xiaofan Song ◽  
Li Ma ◽  
Xina Dou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactococcus Lactis ◽  
Sodium Selenite ◽  
Low Cost ◽  
Intestinal Barrier ◽  
Feed Additives ◽  
Enterotoxigenic Escherichia Coli ◽  
Protective Effects ◽  
Barrier Dysfunction ◽  
Wide Range

Composite microecological agents have received widespread attention due to their advantageous properties, including safety, multi-effects, and low cost. This study was conducted to evaluate the protective effects of selenium (Se) nanoparticle-enriched Lactococcus lactis NZ9000 ( L. lactis NZ9000-SeNPs) against enterotoxigenic Escherichia coli K88 (ETEC K88)-induced intestinal barrier damage in C57BL/6 mice. Oral administration of L. lactis NZ9000-SeNPs significantly increased the villi height and the number of goblet cells in the ileum, and reduced the levels of serum and ileal interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ), and increased the activities of thioredoxin reductase (TrxR) and glutathione peroxidase (GSH-Px) compared with the ETEC K88-infected group not treated with L. lactis NZ9000-SeNPs. In addition, L. lactis NZ9000-SeNPs significantly attenuated the reduction of the expression levels of occludin and claudin-1, dysbiosis of the gut microbiome, and the activation of toll-like receptor (TLR)/nuclear factor-kappa (NF-κB)-mediated signaling pathway induced by ETEC K88. These findings suggested that L. lactis NZ9000-SeNPs may be a promising and safe Se supplement for food or feed additives. Importance The beneficial effects of microecological agents have been widely proven. Se, which is nutritionally essential trace element for human and animals, is incorporated into selenoproteins that have a wide range of pleiotropic effects, ranging from antioxidant and anti-inflammatory effects. However, the sodium selenite, a common addition form of Se in feed and food, has disadvantages such as strong toxicity and low bioavailability. We investigated the protective effects of L. lactis NZ9000-SeNPs against ETEC K88-induced intestinal barrier injury in C57BL/6 mice. Our results show that L. lactis NZ9000-SeNPs effectively alleviate ETEC-K88-induced intestinal barrier dysfunction. This study highlights the importance of developing a promising and safe Se supplement for the substation of sodium selenite applied in food, feed and biomedicine.

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