The Evaluation of TUNEL, PCNA and SOX2 Expressions in Lens Epithelial Cells of Cataract Patients with Pseudoexfoliation Syndrome

2019 ◽  
Vol 45 (1) ◽  
pp. 12-16 ◽  
Author(s):  
Gulay Turan ◽  
Meydan Turan
2014 ◽  
Vol 51 (3) ◽  
pp. 124-128 ◽  
Author(s):  
Junfang Zhang ◽  
Jian Wu ◽  
Ling Yang ◽  
Rongrong Zhu ◽  
Mei Yang ◽  
...  

1995 ◽  
Vol 130 (1) ◽  
pp. 169-181 ◽  
Author(s):  
W C Li ◽  
J R Kuszak ◽  
K Dunn ◽  
R R Wang ◽  
W Ma ◽  
...  

Cataract is a major ocular disease that causes blindness in many developing countries of the world. It is well established that various factors such as oxidative stress, UV, and other toxic agents can induce both in vivo and in vitro cataract formation. However, a common cellular basis for this induction has not been previously recognized. The present study of lens epithelial cell viability suggests such a general mechanism. When lens epithelial cells from a group of 20 cataract patients 12 to 94 years old were analyzed by terminal deoxynucleotidyl transferase (TdT) labeling and DNA fragmentation assays, it was found that all of these patients had apoptotic epithelial cells ranging from 4.4 to 41.8%. By contrast, in eight normal human lenses of comparable age, very few apoptotic epithelial cells were observed. We suggest that cataract patients may have deficient defense systems against factors such as oxidative stress and UV at the onset of the disease. Such stress can trigger lens epithelial cell apoptosis that then may initiate cataract development. To test this hypothesis, it is also demonstrated here that hydrogen peroxide at concentrations previously found in some cataract patients induces both lens epithelial cell apoptosis and cortical opacity. Moreover, the temporal and spatial distribution of induced apoptotic lens epithelial cells precedes development of lens opacification. These results suggest that lens epithelial cell apoptosis may be a common cellular basis for initiation of noncongenital cataract formation.


2021 ◽  
Vol 8 ◽  
Author(s):  
Sofija Andjelic ◽  
Kazimir Drašlar ◽  
Anastazija Hvala ◽  
Marko Hawlina

The purpose of this work is to examine the structure of the anterior lens epithelial cells (aLECs) of presenile idiopathic cortical cataract to investigate the possible structural reasons for its development. The anterior lens capsules (aLCs: basement membrane and associated lens epithelial cells) were obtained from routine uneventful cataract surgery of 5 presenile cataract patients (16 and 41 years old women and 29, 39, and 45 years old men). None of the patients had family history of cataract, medication, or trauma and they were otherwise healthy. In addition, the patients did not have any other abnormal features in the ocular status except cataract. The aLCs were prepared for scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The most prominent abnormal features observed by SEM for all 5 studied presenile cataract patients were the changes of the aLECs structure with the dents, the selective concavity of some LECs, at their apical side centrally toward the nucleus. In addition, TEM showed the thinning of the lens epithelium with the segmentally concave cells and the compressed and elongated nuclei. Abnormal and distinguishable structural features were observed in the anterior lens epithelium aLECs in all 5 patients with presenile cataract. Disturbed structure of aLECs, regularly present in presenile cataract type is shown that might be associated with water accumulation in the presenile idiopathic cortical cataract lens.


2019 ◽  
Vol 97 (S263) ◽  
Author(s):  
Sofija Andjelic ◽  
Martin Kreuzer ◽  
Tanja Dučić ◽  
Marko Hawlina

Eye ◽  
2006 ◽  
Vol 21 (11) ◽  
pp. 1406-1411 ◽  
Author(s):  
S P Gartaganis ◽  
N E Patsoukis ◽  
D K Nikolopoulos ◽  
C D Georgiou

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