Faculty Opinions recommendation of Changes in apoptosis factors in lens epithelial cells of cataract patients with diabetes mellitus.

Purpose: The aim of this study was to evaluate the immunoreactivity of matrix metalloproteinases-2 (MMP-2), intercellular adhesion molecule-1 (ICAM-1), and nesfatin-1 in cataract lens epithelial cells (LECs) of patients with diabetes mellitus (DM) and to investigate the relationship of these markers with DM cataract and diabetic retinopathy (DR). Materials and methods: Ninety patients were included in the study. The patients were divided into three groups ( n = 30): Group 1 (control; patients without DM or DR); Group 2 (patients with DM only), and Group 3 (patients with both DM and DR). Lens capsule samples were collected during intraoperative cataract surgery. Samples were immunohistochemically stained for MMP-2, ICAM-1, and nesfatin-1 and their immunoreactivity was evaluated. The number of immunoreactive cells was determined with a microscope at ×400 magnification. Results: Increased MMP-2 and ICAM-1 immunoreactivity was detected in the LECs of patients with DM, and especially in patients with DR ( p < 0.001, p < 0.001, respectively). Nesfatin-1 immunoreactivity was significantly lower in LECs of diabetic patients ( p < 0.001). The mean of MMP-2 immunoreactive cells were 7.47 ± 8.18, 22.80 ± 15.70, and 34.80 ± 20.85 in Groups 1, 2, and 3, respectively. The mean of ICAM-1 immunoreactive cells were 17.10 ± 9.83, 38.50 ± 23.55, and 56.93 ± 20.94 in Groups 1, 2, and 3, respectively. Conclusion: Nesfatin-1, MMP-2, and ICAM-1 and could potentially play important roles in the pathogenesis of cataracts in patients with DM.

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DNA Damage in Lens Epithelial Cells and Peripheral Lymphocytes from Age-Related Cataract Patients

Ophthalmic Research ◽

10.1159/000356399 ◽

2014 ◽

Vol 51 (3) ◽

pp. 124-128 ◽

Cited By ~ 15

Author(s):

Junfang Zhang ◽

Jian Wu ◽

Ling Yang ◽

Rongrong Zhu ◽

Mei Yang ◽

...

Keyword(s):

Dna Damage ◽

Epithelial Cells ◽

Lens Epithelial Cells ◽

Peripheral Lymphocytes ◽

Age Related ◽

Cataract Patients

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Lens epithelial cell apoptosis appears to be a common cellular basis for non-congenital cataract development in humans and animals.

The Journal of Cell Biology ◽

10.1083/jcb.130.1.169 ◽

1995 ◽

Vol 130 (1) ◽

pp. 169-181 ◽

Cited By ~ 210

Author(s):

W C Li ◽

J R Kuszak ◽

K Dunn ◽

R R Wang ◽

W Ma ◽

...

Keyword(s):

Epithelial Cell ◽

Epithelial Cells ◽

Cell Apoptosis ◽

Lens Epithelial Cell ◽

Lens Epithelial Cells ◽

Cataract Formation ◽

Cellular Basis ◽

Epithelial Cell Apoptosis ◽

Cataract Development ◽

Cataract Patients

Cataract is a major ocular disease that causes blindness in many developing countries of the world. It is well established that various factors such as oxidative stress, UV, and other toxic agents can induce both in vivo and in vitro cataract formation. However, a common cellular basis for this induction has not been previously recognized. The present study of lens epithelial cell viability suggests such a general mechanism. When lens epithelial cells from a group of 20 cataract patients 12 to 94 years old were analyzed by terminal deoxynucleotidyl transferase (TdT) labeling and DNA fragmentation assays, it was found that all of these patients had apoptotic epithelial cells ranging from 4.4 to 41.8%. By contrast, in eight normal human lenses of comparable age, very few apoptotic epithelial cells were observed. We suggest that cataract patients may have deficient defense systems against factors such as oxidative stress and UV at the onset of the disease. Such stress can trigger lens epithelial cell apoptosis that then may initiate cataract development. To test this hypothesis, it is also demonstrated here that hydrogen peroxide at concentrations previously found in some cataract patients induces both lens epithelial cell apoptosis and cortical opacity. Moreover, the temporal and spatial distribution of induced apoptotic lens epithelial cells precedes development of lens opacification. These results suggest that lens epithelial cell apoptosis may be a common cellular basis for initiation of noncongenital cataract formation.

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The Evaluation of TUNEL, PCNA and SOX2 Expressions in Lens Epithelial Cells of Cataract Patients with Pseudoexfoliation Syndrome

Current Eye Research ◽

10.1080/02713683.2019.1657463 ◽

2019 ◽

Vol 45 (1) ◽

pp. 12-16 ◽

Cited By ~ 1

Author(s):

Gulay Turan ◽

Meydan Turan

Keyword(s):

Epithelial Cells ◽

Pseudoexfoliation Syndrome ◽

Lens Epithelial Cells ◽

Cataract Patients

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Structural Characteristics of the Lens in Presenile Cataract

Frontiers in Medicine ◽

10.3389/fmed.2021.802275 ◽

2021 ◽

Vol 8 ◽

Author(s):

Sofija Andjelic ◽

Kazimir Drašlar ◽

Anastazija Hvala ◽

Marko Hawlina

Keyword(s):

Electron Microscopy ◽

Epithelial Cells ◽

Structural Characteristics ◽

Structural Features ◽

Lens Epithelium ◽

Lens Epithelial Cells ◽

Cortical Cataract ◽

Apical Side ◽

Cataract Patients ◽

Presenile Cataract

The purpose of this work is to examine the structure of the anterior lens epithelial cells (aLECs) of presenile idiopathic cortical cataract to investigate the possible structural reasons for its development. The anterior lens capsules (aLCs: basement membrane and associated lens epithelial cells) were obtained from routine uneventful cataract surgery of 5 presenile cataract patients (16 and 41 years old women and 29, 39, and 45 years old men). None of the patients had family history of cataract, medication, or trauma and they were otherwise healthy. In addition, the patients did not have any other abnormal features in the ocular status except cataract. The aLCs were prepared for scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The most prominent abnormal features observed by SEM for all 5 studied presenile cataract patients were the changes of the aLECs structure with the dents, the selective concavity of some LECs, at their apical side centrally toward the nucleus. In addition, TEM showed the thinning of the lens epithelium with the segmentally concave cells and the compressed and elongated nuclei. Abnormal and distinguishable structural features were observed in the anterior lens epithelium aLECs in all 5 patients with presenile cataract. Disturbed structure of aLECs, regularly present in presenile cataract type is shown that might be associated with water accumulation in the presenile idiopathic cortical cataract lens.

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