Evaluation of an indirect enzyme-linked immunosorbent assay to study the specific humoral immune response of Muscovy ducks (Cairina moschata) and domestic geese (Anser anser var. domestica) after vaccination against Newcastle disease virus

The study was carried out to determine the humoral immune response to Newcastle Disease Virus (NDV) and Bangladesh Agricultural University Fowl cholera (BAUFC) vaccines in Shuvra chicken, a newly develop chicken strain by BLRI (Bangladesh Livestock Research Institute). Ten Shuvra chickens were vaccinated with Baby Chick Ranikhet Disease Vaccine (BCRDV) at day 7 through intra ocular route (i/o) and with Ranikhet Disease Vaccine (RDV) at day 35 through intramuscular (i/m) route. Vaccine induced serum Haemagglunination Inhibition (HI) antibodies were measured by HI test. Two weeks after final immunization all vaccinated and control Shuvra chickens were challenged with virulent field isolates of NDV where all the vaccinated birds survived without showing any typical signs of NDV during the period of ten days observation period and all the control chickens died. Another 10 Shuvra were vaccinated twice with BAUFC vaccine through intramuscular route at day 42 and 70, and 10 Shuvra chickens were kept as unvaccinated control. This vaccine also induced significantly higher level of antibody titre as determined by Passive Haemagglutination (PHA) test. Vaccinated chickens showed significantly higher survival (80%) following challenge with virulent fowl cholera isolate and all the control birds died within 10 days of observation period. DOI: http://dx.doi.org/10.3329/bjvm.v10i1-2.15639Bangl. J. Vet. Med. (2012). 10 (1&2): 1-7

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Optimization of Human Immunodeficiency Virus Gag Expression by Newcastle Disease Virus Vectors for the Induction of Potent Immune Responses

Journal of Virology ◽

10.1128/jvi.01443-08 ◽

2008 ◽

Vol 83 (2) ◽

pp. 584-597 ◽

Cited By ~ 61

Author(s):

Elena Carnero ◽

Wenjing Li ◽

Antonio V. Borderia ◽

Bruno Moltedo ◽

Thomas Moran ◽

...

Keyword(s):

Immune Response ◽

Human Immunodeficiency Virus ◽

Immune Responses ◽

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

Insertion Site ◽

Gag Protein ◽

Immunodeficiency Virus

ABSTRACT One attractive strategy for the development of a human immunodeficiency virus (HIV) vaccine is the use of viral vectors with a proven safety profile and an absence of preexisting immunity in humans, such as Newcastle disease virus (NDV). Several NDV vaccine vectors have been generated, and their immunogenicities have been investigated with different animal models. However, a systematic study to evaluate the optimal insertion site of the foreign antigens into NDV that results in enhanced immune responses specific to the antigen has not yet been conducted. In this article, we describe the ability of NDV expressing HIV Gag to generate a Gag-specific immune response in mice. We also have determined the optimal insertion site into the NDV genome by generating recombinant NDV-HIVGag viruses in which HIV gag was located at different transcriptional positions throughout the NDV viral genome. All recombinant viruses were viable, grew to similar titers in embryonated chicken eggs, and expressed Gag in a stable manner. Our in vivo experiments revealed that higher HIV Gag protein expression positively correlates with an enhanced CD8+ T-cell-mediated immune response and protective immunity against challenge with vaccinia virus expressing HIV Gag. We also inserted a codon-optimized version of HIV gag in the described best location, between the P and M genes. Virus expressing the codon-optimized version of HIV gag induced a higher expression of the protein and an enhanced immune response against HIV Gag in mice. These results indicate that strategies directed toward increasing antigen expression by NDV result in enhanced immunogenicity and vaccine efficacy.

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Immune Response of Chickens to Six Strains of Newcastle Disease Virus as Measured by Hemagglutination-Inhibition Test

Avian Diseases ◽

10.2307/1588413 ◽

1969 ◽

Vol 13 (1) ◽

pp. 46

Author(s):

P. W. Chang ◽

V. J. Yates ◽

J. A. Pendleton ◽

T. D. Flanagan

Keyword(s):

Immune Response ◽

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

Hemagglutination Inhibition ◽

Inhibition Test ◽

Hemagglutination Inhibition Test

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Mucosal Immunization with Newcastle Disease Virus Vector Coexpressing HIV-1 Env and Gag Proteins Elicits Potent Serum, Mucosal, and Cellular Immune Responses That Protect against Vaccinia Virus Env and Gag Challenges

mBio ◽

10.1128/mbio.01005-15 ◽

2015 ◽

Vol 6 (4) ◽

Cited By ~ 21

Author(s):

Sunil K. Khattar ◽

Vinoth Manoharan ◽

Bikash Bhattarai ◽

Celia C. LaBranche ◽

David C. Montefiori ◽

...

Keyword(s):

Immune Responses ◽

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

Effective Vaccine ◽

Gag Proteins ◽

Humoral Immune ◽

Vaccinia Viruses ◽

Mucosal Immune Responses ◽

Hiv 1

ABSTRACT Newcastle disease virus (NDV) avirulent strain LaSota was used to coexpress gp160 Env and p55 Gag from a single vector to enhance both Env-specific and Gag-specific immune responses. The optimal transcription position for both Env and Gag genes in the NDV genome was determined by generating recombinant NDV (rNDV)-Env-Gag (gp160 located between the P and M genes and Gag between the HN and L genes), rNDV-Gag-Env (Gag located between the P and M genes and gp160 between the HN and L genes), rNDV-Env/Gag (gp160 followed by Gag located between the P and M genes), and rNDV-Gag/Env (Gag followed by gp160 located between the P and M genes). All the recombinant viruses replicated at levels similar to those seen with parental NDV in embryonated chicken eggs and in chicken fibroblast cells. Both gp160 and Gag proteins were expressed at high levels in cell culture, with gp160 found to be incorporated into the envelope of NDV. The Gag and Env proteins expressed by all the recombinants except rNDV-Env-Gag self-assembled into human immunodeficiency virus type 1 (HIV-1) virus-like particles (VLPs). Immunization of guinea pigs by the intranasal route with these rNDVs produced long-lasting Env- and Gag-specific humoral immune responses. The Env-specific humoral and mucosal immune responses and Gag-specific humoral immune responses were higher in rNDV-Gag/Env and rNDV-Env/Gag than in the other recombinants. rNDV-Gag/Env and rNDV-Env/Gag were also more efficient in inducing cellular as well as protective immune responses to challenge with vaccinia viruses expressing HIV-1 Env and Gag in mice. These results suggest that vaccination with a single rNDV coexpressing Env and Gag represents a promising strategy to enhance immunogenicity and protective efficacy against HIV. IMPORTANCE A safe and effective vaccine that can induce both systemic and mucosal immune responses is needed to control HIV-1. In this study, we showed that coexpression of Env and Gag proteins of HIV-1 performed using a single Newcastle disease virus (NDV) vector led to the formation of HIV-1 virus-like particles (VLPs). Immunization of guinea pigs with recombinant NDVs (rNDVs) elicited potent long-lasting systemic and mucosal immune responses to HIV. Additionally, the rNDVs were efficient in inducing cellular immune responses to HIV and protective immunity to challenge with vaccinia viruses expressing HIV Env and Gag in mice. These results suggest that the use of a single NDV expressing Env and Gag proteins simultaneously is a novel strategy to develop a safe and effective vaccine against HIV.

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