Interaction of Bacillus subtilis with resistant and susceptible tomato (Solanum lycopersicum L.) in the control of Meloidogyne incognita

2020 ◽  
pp. 1-16
Author(s):  
Lorrayne Guimarães Bavaresco ◽  
Luciana Machado Guaberto ◽  
Fábio Fernando Araujo
Keyword(s):  
Bacillus Subtilis ◽  
Solanum Lycopersicum ◽  
Meloidogyne Incognita ◽  
Solanum Lycopersicum L ◽  
Susceptible Tomato

scholarly journals Effect of Individual, Simultaneous and Sequential Inoculation of Pseudomonas fluorescens and Meloidogyne incognita on Growth, Biochemical, Enzymatic and Nonenzymatic Antioxidants of Tomato (Solanum lycopersicum L.)

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1145
Author(s):  
Ahmed Noureldeen ◽  
Mohd Asif ◽  
Taruba Ansari ◽  
Faryad Khan ◽  
Mohammad Shariq ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Pseudomonas Fluorescens ◽  
Solanum Lycopersicum ◽  
Meloidogyne Incognita ◽  
Interactive Effects ◽  
Nematode Reproduction ◽  
Solanum Lycopersicum L ◽  
Defensive Enzymes ◽  
Sequential Inoculation ◽  
Nonenzymatic Antioxidants

This study was conducted on tomato (Solanum lycopersicum cv. K-21) to investigate the bioprotective nature of Pseudomonas fluorescens and its interactive effects with Meloidogyne incognita in terms of growth biomarkers, changes in biochemical attributes and modulation in antioxidant enzymes of the tomato plant. In this study, we grew tomato plants with M. incognita and P. fluorescens in separate pots, simultaneously and sequentially (15 days prior or post) after 15 days of seed sowing. The sequential inoculation of Mi15→Pf maximally increased the root-knot index and decreased the nematode population. It was also noted that inoculation suppressed the plant growth biomarkers in comparison to control. However, maximum suppression in nematode reproduction and increment in growth and physiological attributes were observed when P. fluorescens was applied 15 days prior to the nematode (Pf15→Mi) as compared to control. All the treatments showed an increase in antioxidant enzymes. Expression of phenol content and defensive enzymes such as peroxidase (POX) and superoxide dismutase (SOD) increased, in contrast to a significant reduction in malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents when compared with the untreated inoculated plants. However, the highest levels of POX and SOD, and a lowest of phenol, MDA and H2O2 were displayed in the treatment Pf15→Mi, followed by Mi+Pf and Mi15→Pf.

scholarly journals Evaluación de inductores de resistencia al TMV en tomate

2020 ◽  
Vol 11 (2) ◽  
pp. 377-390
Author(s):  
Ramiro Hernández Santiago ◽  
Mateo Vargas Hernández ◽  
Erika Janet Zamora Macorra
Keyword(s):  
Bacillus Subtilis ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L ◽  
Das Elisa

Se realizaron aplicaciones de los inductores de resistencia: Messenger gold®, Virus Stop®, Actigard®, Virablock®, Kendal®, fosfito de potasio, Stymulus® Maxx, Bacillus subtilis y MC Cream® en el año 2018, de forma individual y combinados en secuencias, para evaluar su efecto sobre la concentración viral (densidad óptica), crecimiento, longitud de raíz, severidad, peso seco total, número y peso de frutos en el cultivo de jitomate (Solanum lycopersicum L.) infectado con Tobacco mosaic virus (TMV). La secuencia de los inductores Messenger gold®+ Messenger gold®+ MC Cream® + MC Cream® redujeron la concentración de TMV a los 38 días después de la inoculación, determinada mediante DAS-ELISA. La aspersión de Virablock® obtuvo la mayor altura promedio. Las plantas tratadas con la secuencia Virus Stop® + Virus Stop® + fosfito de potasio + fosfito de potasio obtuvieron la menor severidad promedio. Las plantas asperjadas con Messenger gold® + Messenger gold® + MC Cream® + MC Cream® registraron la mayor longitud de raíz, mayor peso seco total y el segundo mayor número y peso promedio de frutos que no mostraron síntomas de TMV en frutos.

scholarly journals Bioefficacy Study of Bacillus subtilis Based Biofungicide on Leaf Spot Disease, Growth and Yield Attributes of Tomato [Solanum lycopersicum L.] CV. Arka Vikas

2020 ◽  
pp. 35-41
Author(s):  
Ajay Kumar Tiwari ◽  
B. K. Singh ◽  
Trivikram . ◽  
A. K. Singh ◽  
Pankaj Kumar Singh
Keyword(s):  
Bacillus Subtilis ◽  
Solanum Lycopersicum ◽  
Leaf Spot ◽  
Fruit Yield ◽  
Growth And Yield ◽  
Leaf Spot Disease ◽  
Yield Attributes ◽  
Spot Disease ◽  
Solanum Lycopersicum L ◽  
Yield Parameters

The present study entitled “Bioefficacy study of Bacillus subtilis based biofungicide on leaf spot disease, growth and yield attributes of tomato [Solanum lycopersicum L.] cv. ArkaVikas” was conducted at Vegetable Research Farm, Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi during rainy season of 2016-17 in Randomized Block Design (RBD) with three replications. The treatments included foliar spray of chemical fungicide (Copper oxychlooride) and biofungicide (Taegro® and Trichoderma) either alone or in combination. Taegro® is a bacterial biofungicide containing 1×1010 CFU/g (13%w/w) of Bacillus amyloliuefaciens strain FZB24 formulated as WP. A total of 12 characters including disease, growth and yield parameters were studied. Disease parameter included leaf spot disease incidence (%) and disease severity (%) whereas growth and yield parameters included days to 50% flowering, height, number of branches per plant, fruit length (cm), fruit width (cm), number of fruits per plant, average fruit weight (g), number of seed per fruit, fruit yield per plot (kg), fruit yield per hectare (kg) were studied. The biofungicide Taegro exhibited significant potential in reducing the leaf spot in tomato and improving the growth and yield attributes of tomato as compared to control. But combined used of Taegro with standard chemical copper oxychloride gave better result than Taegro alone. As a consequence, this may be used as part of an integrated disease management approach so as to minimizes the use of standard fungicides and also protect the environment from pollution and maintenance of the human health.

scholarly journals Especies nativas de Trichoderma spp. y su actividad antagónica contra Meloidogyne incognita en Solanum lycopersicum L.

2018 ◽  
pp. 5-12
Author(s):  
Jiram Ivan Cetz Chi ◽  
Jairo Cristóbal Alejo ◽  
José María Tún Suárez ◽  
Fernando Antonio Peraza Luna ◽  
Juan Candelero De la Cruz
Keyword(s):  
Solanum Lycopersicum ◽  
Meloidogyne Incognita ◽  
Trichoderma Spp ◽  
Solanum Lycopersicum L

En condiciones protegidas, se evaluó el potencial antagónico de cinco especies nativas de Trichoderma; T. harzianum (Th02-04), T. harzianum (Th10-D86), T. simmonsi (Th33-58), T. virens (Th33-59) y T. virens  (Th26-52), especies comerciales y un testigo sin inoculantes fúngicos, distribuidas en un diseño completamente al azar y cuatro repeticiones en plántulas de tomate (Solanum lycopersicum L.), inoculadas con tres aplicaciones de 1 x106 conidias ml-1. Se inocularon 18, 500 huevos de M. incognita y 10 ml con la concentración inicial de las conidias fúngicas, con aplicaciones al momento, a los ocho y 15 días después del trasplante. El producto comercial y las especies T. virens (Th26-52 y Th3359) lograron disminuir hasta 22% la formación de agallas, 87 y 52.39% de inhibición de producción de formación de hembras por gramo de raíz. El control de estas especies contra el nematodo favoreció el mejor crecimiento vegetativo de las plantas.

scholarly journals Variations in Growth, Physiology, and Antioxidative Defense Responses of Two Tomato (Solanum lycopersicum L.) Cultivars after Co-Infection of Fusarium oxysporum and Meloidogyne incognita

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 159 ◽  
Author(s):  
Ambreen Maqsood ◽  
Haiyan Wu ◽  
Muhammad Kamran ◽  
Hussain Altaf ◽  
Adnan Mustafa ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Solanum Lycopersicum ◽  
Meloidogyne Incognita ◽  
Photosynthetic Capacity ◽  
Dry Weight ◽  
Defense Responses ◽  
Root Cells ◽  
Chlorophyll Contents ◽  
Growth Physiology ◽  
Solanum Lycopersicum L

The soil-borne fungus Fusarium oxysporum (Fo) and the nematode Meloidogyne incognita (Mi) are destructive pathogens that cause substantial yield losses to tomato (Solanum lycopersicum L.) crops worldwide. The present study sought to elucidate the physiological, biochemical, and cytological responses of tomato cultivars (Gailing maofen 802 and Zhongza 09) by root invasion of Fo (1 × 105 CFUmL−1) and Mi (1500 second-stage juveniles (J2) alone and in combination after 14 days. Results revealed that combined inoculation of Fo and Mi significantly increased disease intensity, electrolyte leakage, and hydrogen peroxide and malondialdehyde contents; and decreased photosynthetic capacity and enzyme activity in both cultivars as compared to their solo inoculation. Increasing the disease intensity reduced the maximum morphological traits, such as shoot length, total dry weight, and total chlorophyll contents, in G. maofen 802 (by 32%, 54.2%, and 52.3%, respectively) and Zhongza 09 (by 18%, 32%, and 21%, respectively) as compared to the control. Others factors were also reduced in G. maofen 802 and Zhongza 09, such as photosynthetic capacity (by 70% and 57%, respectively), stomatal conductance (by 86% and 70%, respectively), photochemical quantum yield of photosystem II (YII) (by 36.6% and 29%, respectively), and electron transport rate (by 17.7% and 10%, respectively), after combined inoculation of Fo and Mi. Furthermore, the combined infestation of Fo and Mi resulted in reduced activity of plant-defense-related antioxidants in G. maofen 802 compared with their single application or control. However, these antioxidants were highly up-regulated in Zhongza 09 (by 59%–93%), revealing the induction of tolerance against studied pathogens. The transmission electron microscopy (TEM) results further demonstrated that root cells of Zhongza 09 had unique tetrahedral crystal-like structures in the membrane close to mitochondria under all treatments except control. Therefore, it is concluded that Mi caused severe root damage, suppressed plant growth, depleted antioxidants, and caused high generation of ROS in the presence of Fo as compared to its solo inoculation. Tolerant cultivars adopted different mechanistic strategies at the structural and cellular levels to tolerate the Mi and Fo stresses.

scholarly journals Respuesta de quince introducciones de tomate cherry (Solanum lycopersicum L.) al nematodo nodulador (Meloidogyne spp. GOELDI) e identificación de las especies

2016 ◽  
Vol 40 (156) ◽  
pp. 450
Author(s):  
Luisa Fernanda Cardona-Piedrahíta ◽  
Jairo Castaño-Zapata ◽  
Nelson Ceballos Aguirre
Keyword(s):  
Solanum Lycopersicum ◽  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Solanum Lycopersicum L ◽  
El Sistema ◽  
Meloidogyne Spp

<p>Meloidogyne spp. es un problema de gran importancia económica en el cultivo de tomate tipo cherry, causando pérdidas en rendimiento del 25 al 50%, lo cual conlleva a la búsqueda de materiales silvestres resistentes o promisorios. El objetivo de este trabajo fue contribuir en el conocimiento de la epidemiología del nematodo del Nudo radical, causado por Meloidogyne spp. en quince introducciones de tomate silvestre tipo cereza sembrados a libre exposición y bajo semitecho y, determinar las especies de Meloidogyne presentes en el cultivo. Se utilizó un diseño de parcelas divididas, las parcelas principales fueron asignadas a los dos sistemas de producción (libre exposición y semitecho), mientras que las subparcelas fueron asignadas a las introducciones de tomate, con cuatro repeticiones. El tamaño efectivo de la unidad experimental fue de cuatro plantas por repetición. Se evaluó el daño radical causado por el nematodo y se hizo identificación de especies tanto en hembras como en juveniles (J2). Se realizó un análisis de varianza y pruebas de promedio tipo Duncan. En el sistema productivo a libre exposición la población de nematodos osciló entre 29.472 en IAC1624 y 170.804 en IAC421 con un promedio de 97.677 nematodos y en el sistema bajo semitecho, osciló entre 57.871 en IAC391 y 197.800 en LA2692 con un promedio de 108.572 nematodos, lo que demuestra que este sistema reduce el ciclo del nematodo, y por consiguiente, aumenta la población. Las especies identificadas según el método del patrón perineal sobre las hembras fueron Meloidogyne incognita (80%) y Meloidogyne javanica (20%). © 2016. Acad. Colomb. Cienc. Ex. Fis. Nat.</p>

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