scholarly journals Note on the Extraction of Silk-Fibroin by Formic Acid

1926 ◽  
Vol 2 (7) ◽  
pp. 81-82
Author(s):  
Tokuhei Kametaka
Keyword(s):  
Formic Acid ◽  
Silk Fibroin

Large‐area thin films of silk fibroin prepared by two methods with formic acid as solvent and glycerol as plasticizer

2021 ◽  
pp. 50759
Author(s):  
Francisco Vieira Santos ◽  
Sergio Akinobu Yoshioka ◽  
Marcia Cristina Branciforti
Keyword(s):  
Thin Films ◽  
Formic Acid ◽  
Silk Fibroin ◽  
Large Area

scholarly journals Effect of Na2CO3 degumming concentration on LiBr-formic acid-silk fibroin solution properties

2016 ◽  
Vol 20 (3) ◽  
pp. 985-991
Author(s):  
Zhi Liu ◽  
Yuqin Wan ◽  
Hao Dou ◽  
Ji-Huan He
Keyword(s):  
Formic Acid ◽  
Silk Fibroin ◽  
Sodium Carbonate ◽  
High Efficiency ◽  
Lithium Bromide ◽  
Solution Properties ◽  
Acid System ◽  
Acid Dissolution ◽  
Carbonate Concentration ◽  
Degumming Process

Salt-acid system has been proved to be of high efficiency for silk fibroin dissolution. Using salt-acid system to dissolve silk, native silk fibrils can be preserved in the regenerated solution. Increasing experiments indicate that acquirement of silk fibrils in solution is strongly associated with the degumming process. In this study, the effect of sodium carbonate degumming concentration on solution properties based on lithium bromide-formic acid dissolution system was systematically investigated. Results showed that the morphology transformation of silk fibroin in solution from nanospheres to nanofibrils is determined by sodium carbonate concentration during the degumming process. Solutions containing different silk fibroin structure exhibited different rheological behaviors and different electrospinnability, leading to different electrospun nanofibre properties. The results have guiding significance for preparation and application of silk fibroin solutions.

scholarly journals Fabrication of Silk Nanofibres with Needle and Roller Electrospinning Methods

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Nongnut Sasithorn ◽  
Lenka Martinová
Keyword(s):  
Formic Acid ◽  
Production Rate ◽  
Calcium Chloride ◽  
Silk Fibroin ◽  
Applied Voltage ◽  
Rotating Cylinder ◽  
Spinning Process

In this study, silk nanofibres were prepared by electrospinning from silk fibroin in a mixture of formic acid and calcium chloride. A needle and a rotating cylinder were used as fibre generators in the spinning process. The influences of the spinning electrode and spinning parameters (silk concentration and applied voltage) on the spinning process, morphology of the obtained fibres, and the production rate of the spinning process were examined. The concentration of the spinning solution influenced the diameter of the silk electrospun fibres, with an increase in the concentration increasing the diameters of the fibres in both spinning systems. The diameters of the electrospun fibres produced by roller electrospinning were greater than those produced by needle electrospinning. Moreover, increasing the concentration of the silk solution and the applied voltage in the spinning process improved the production rate in roller electrospinning but had less influence on the production rate in needle electrospinning.

Effect of Solvent on the Characteristics of Electrospun Regenerated Silk Fibroin Nanofibers

2007 ◽  
Vol 342-343 ◽  
pp. 813-816 ◽  
Author(s):  
Lim Jeong ◽  
Kuen Yong Lee ◽  
Won Ho Park
Keyword(s):  
Secondary Structure ◽  
Formic Acid ◽  
Silk Fibroin ◽  
Structural Changes ◽  
Effective Means ◽  
Electrospun Nanofibers ◽  
Random Coil ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
Β Sheet

Nonwoven nanofiber matrices were prepared by electrospinning a solution of silk fibroin (SF) dissolved either in formic acid or in 1,1,1,3,3,3-hexafluoro-2-isopropyl alcohol (HFIP). The mean diameter of the electrospun nanofibers prepared from SF dissolved in formic acid was 80 nm with a unimodal size distribution, which was smaller than those prepared from HFIP (380 nm). SF nanofibers were then treated with an aqueous methanol solution, and structural changes due to solvent-induced crystallization of SF were investigated using IR and 13C solid-state CP/MAS NMR spectroscopy. SF nanofibers prepared from formic acid were found to have a higher proportion of β-sheet conformations than those prepared from HFIP. Methanol treatment provided a fast and effective means to alter the secondary structure of both types of SF nanofibers from a random coil form to a β-sheet form. As demonstrated in the present study, this approach to controlling the dimensions and secondary structure of proteins using various solvents may be useful for the design and tailoring of materials for biomedical applications, especially for tissue engineering applications.

Study on SF Nanofibrous Mats via Electrospun with Different Spinning Solvent as TFA/DCM and FA

2013 ◽  
Vol 662 ◽  
pp. 113-116
Author(s):  
Sha Sha Guo ◽  
Xiang Yu Jin ◽  
Qin Fei Ke ◽  
Yun Qian Cao
Keyword(s):  
Formic Acid ◽  
Silk Fibroin ◽  
Electrospun Nanofibers ◽  
Ftir Spectra ◽  
Solution Viscosity ◽  
Sem Images ◽  
Trifluoracetic Acid

Silk fibroin (SF) was regenerated for electrospinning. A mixture solvents Trifluoracetic acid (TFA) and dichloromethane (DCM) was selected as the solvent of regenerated SF. Its spinning properties and characteristics of end nanofibers mats were compared with those of the regenerated SF electrospinning solution dissolved in formic acid (FA). From the comparison of SEM images of regenerated SF electrospinning from the two different solvents solutions, it could be found that the spinning concentration range in TFA&DCM was smaller than that in FA for the solution viscosity was bigger than that in FA. Moreover, from the FTIR spectra of electrospun nanofibers obtained from TFA&DCM and FA was both similar with that of regenerated SF. This meant that there was no new group formed when TFA& DCM was selected as solvents. Therefore, TFA&DCM were also a nice option for the electrospinning of regenerated SF.

Comparisons between silk fibroin nonwoven electrospun fabrics using aqueous and formic acid solutions

2017 ◽  
Vol 67 (7) ◽  
pp. 462-467 ◽  
Author(s):  
Yuki Kishimoto ◽  
Takanori Kobashi ◽  
Shigeru Yamanaka ◽  
Hideaki Morikawa ◽  
Yasushi Tamada
Keyword(s):  
Formic Acid ◽  
Silk Fibroin ◽  
Acid Solutions

Chemical Crosslinking of Silk Fibroin, Chitosan and Gelatin Blend Nanofiber Mats

2016 ◽  
Vol 695 ◽  
pp. 273-277 ◽  
Author(s):  
Pornpen Siridamrong ◽  
Penchom Phrotjanatharee ◽  
Niyom Thamronganaskul
Keyword(s):  
Formic Acid ◽  
Silk Fibroin ◽  
Room Temperature ◽  
Chemical Crosslinking ◽  
Electrospinning Technique ◽  
Blend Composition ◽  
Nanofiber Mats ◽  
Crosslinking Agents

Silk fibroin (SF), chitosan (C), and gelatin (G) blend composition in formic acid have been manufactured by using electrospinning technique in our previous work. The SF: G: C at weight ratios of 10: 20: 1 and 20: 10: 1 (%wt: %wt: ml) were used for crosslinking testing. Glutaraldehyde (GA), and 1-ethyl-3-(3-dimethylaminopropyl) cabodiimide (EDC) / N-hydroxy succcinimide (NHS) (EDC/NHS) were chose as crosslinking agents. All samples were treated in such agents by fumigation for 72 hours followed by dipping for 10 minutes. Then those samples were washed in distill water for 3 times (1 time per 10 minutes) and dried in desiccator at room temperature. GA caused immediately shrink in both nanofiber mats and became clearly visible yellowish. However, a little shrinkage occurred after dipping in EDC/NHS. It possibly concluded that the EDC / NHS appropriated for SF: G: C blend nanofiber mats.

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