Seedling resistance toRhizoctoniaandPythiumspp. in wheat chromosome group 4 addition lines fromThinopyrumspp.

To produce rye–wheat addition lines, 21-chromosome rye–wheat hybrids were produced by crossing tetraploid triticale with diploid rye. The subsequent selfing of the hybrids lead to monosomic and double monosomic wheat additions to rye. Screening of the progeny was carried out using the C-banding technique. The wheat additions can be fertile. Wheat chromosome 6B was the chromosome most frequently added to rye. Ribosomal spacer probe pTa250.4 was used to confirm the results obtained by C-banding for the 6B wheat additions to rye. Embryos of the 21-chromosome rye–wheat hybrids showed a good potential for propagating more plantlets after they had been transferred to artificial medium.Key words: rye–wheat addition lines.

Download Full-text

Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect

Theoretical and Applied Genetics ◽

10.1007/s00122-005-1972-1 ◽

2005 ◽

Vol 110 (7) ◽

pp. 1315-1323 ◽

Cited By ~ 92

Author(s):

M. Mori ◽

N. Uchino ◽

M. Chono ◽

K. Kato ◽

H. Miura

Keyword(s):

Wheat Chromosome ◽

Combined Effect ◽

Grain Dormancy ◽

Group 4 ◽

Chromosome 3A

Download Full-text

Chromosomal location of genes controlling lactate dehydrogenase in rye, wheat, and barley

Genome ◽

10.1139/g92-005 ◽

1992 ◽

Vol 35 (1) ◽

pp. 32-34 ◽

Cited By ~ 2

Author(s):

Volker Thiele ◽

Gilbert Melz

Keyword(s):

Lactate Dehydrogenase ◽

Chinese Spring ◽

Chromosomal Location ◽

Standard Addition ◽

Wheat Chromosome ◽

Gene Location ◽

Group 4 ◽

Cereal Species ◽

Chromosome 4H ◽

Ldh Isozymes

The chromosomal location of lactate dehydrogenease (LDH) genes in rye, barley, and wheat was investigated using the disomic standard addition sets of 'Chinese Spring' – 'Imperial' (wheat–rye) and 'Chinese Spring' – 'Betzes (wheat–barley) and the monosomic rye–wheat additions, respectively. Gel electrophoresis was performed on 5% polyacrylamide gels. Diagnostic bands for LDH isozymes were present only in zone 2. Genes controlling LDH isozymes in rye were found to be located on rye chromosome 4R (Ldh-R1and Ldh-R2) and in barley on chromosome 4Hv (Ldh-H1 and Ldh-H2). Because of the incomplete rye–wheat addition set, only one gene for LDH isozymes of wheat could be located (wheat chromosome 4B; Ldh-B1). In the three cereal species investigated, LDH genes are located generally on homoeologous group 4 chromosomes.Key words: gene location, lactate dehydrogenase, Secale cereale, Triticum aestivum, Hordeum vulgare.

Download Full-text

A New Source of Resistance to Tapesia yallundae Associated with a Homoeologous Group 4 Chromosome in Thinopyrum ponticum

Phytopathology ◽

10.1094/phyto.2004.94.9.932 ◽

2004 ◽

Vol 94 (9) ◽

pp. 932-937 ◽

Cited By ~ 12

Author(s):

H. J. Li ◽

M. Arterburn ◽

S. S. Jones ◽

T. D. Murray

Keyword(s):

Pacific Northwest ◽

Wheat Chromosome ◽

Polymerase Chain Reaction Analysis ◽

The United States ◽

Homoeologous Group ◽

Thinopyrum Ponticum ◽

Eyespot Resistance ◽

Group 4 ◽

Tapesia Yallundae ◽

The Pacific

Wheat (Thinopyrum ponticum line SS767; PI 611939) with 42 chromosomes previously was identified as a new source of eyespot resistance. Individual plants of SS767 were tested for reaction to Tapesia yallundae, the major pathogen of eyespot in the Pacific Northwest region of the United States. Resistance of this line was similar to the resistant winter wheat cv. Madsen (carrying gene Pch1 for eyespot resistance). Polymerase chain reaction analysis with primers specific for the J or E genomes revealed that SS767 contains Thinopyrum chromatin. Cytological and Cbanding analyses demonstrated that SS767 is a chromosome substitution line in which wheat chromosome 4D is replaced by a homoeologous group 4 chromosome of Thinopyrum ponticum. Genomic in situ hybridization using St genomic DNA from Pseudoroegneria strigosa as a probe, which can differentiate chromosomes from different genomes of Thinopyrum, indicated that this chromosome belongs to the J genome. Molecular analysis of an F2 population segregating for chromosome 4J and resistance to eyespot confirmed that eyespot resistance in line SS767 is associated with chromosome 4J of Thinopyrum ponticum. This is the first report of genetic control of resistance to eyespot derived from Thinopyrum ponticum. This source of resistance provides a new opportunity to improve wheat resistance to eyespot by adding to the diversity of resistance sources available.

Download Full-text

Chromosomal location of three wheat sequences with homology to pollen allergen encoding, DNA replication regulating, and DNA (cytosine-5)-methyltransferase genes in wheat and rye

Genome ◽

10.1139/g96-154 ◽

1996 ◽

Vol 39 (6) ◽

pp. 1213-1215 ◽

Cited By ~ 2

Author(s):

V. Korzun ◽

H.-J. Balzer ◽

A. Balzer ◽

H. Bäumlein ◽

A. Börner

Keyword(s):

Dna Replication ◽

Key Words ◽

Chinese Spring ◽

Chromosomal Location ◽

Chromosomal Rearrangements ◽

Pollen Allergen ◽

Addition Lines ◽

Homoeologous Group ◽

Group 4

Three wheat sequences, shown to be homologous to pollen allergen encoding, DNA replication regulating, and DNA (cytosine-5)-methyltransferase genes were localized on chromosomes using nullisomic–tetrasomic wheat ('Chinese Spring') and wheat–rye ('Chinese Spring'/'Imperial') addition lines. Whereas the loci for the pollen allergen encoding sequence (Tri a III) were shown to be located on homoeologous group 4, the DNA replication regulating (Rep) and DNA (cytosine-5)-methyltransferase (Mtase) genes were located to homoeologous groups 1 and 7, respectively, of Triticeae. Chromosomal rearrangements in wheat and rye relative to each other are discussed. Key words : chromosomal location, known function probes, RFLP, rye, wheat.

Download Full-text

Morphological Changes in the Rat Granulosa Cell Surface During Differentiation Induced by Estradiol and Gonadotropins

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079851 ◽

1977 ◽

Vol 35 ◽

pp. 484-485

Author(s):

P. Bagavandoss ◽

JoAnne S. Richards ◽

A. Rees Midgley

Keyword(s):

Cell Surface ◽

Granulosa Cell ◽

Morphological Changes ◽

Follicular Development ◽

Female Rats ◽

Functional Changes ◽

Group 4 ◽

Group 3 ◽

Group 5 ◽

Group 2

During follicular development in the mammalian ovary, several functional changes occur in the granulosa cells in response to steroid hormones and gonadotropins (1,2). In particular, marked changes in the content of membrane-associated receptors for the gonadotropins have been observed (1).We report here scanning electron microscope observations of morphological changes that occur on the granulosa cell surface in response to the administration of estradiol, human follicle stimulating hormone (hFSH), and human chorionic gonadotropin (hCG).Immature female rats that were hypophysectcmized on day 24 of age were treated in the following manner. Group 1: control groups were injected once a day with 0.1 ml phosphate buffered saline (PBS) for 3 days; group 2: estradiol (1.5 mg/0.2 ml propylene glycol) once a day for 3 days; group 3: estradiol for 3 days followed by 2 days of hFSH (1 μg/0.1 ml) twice daily, group 4: same as in group 3; group 5: same as in group 3 with a final injection of hCG (5 IU/0.1 ml) on the fifth day.

Download Full-text