Catechin isolated from faba beans (Vicia faba L.): insights from oxidative stress and hypoglycemic effect in yeast cells through confocal microscopy, flow cytometry, and in silico strategy

2021 ◽  
pp. 1-11
Author(s):  
Dhiraj Kumar Choudhary ◽  
Navaneet Chaturvedi ◽  
Amit Singh ◽  
Abha Mishra
Keyword(s):  
Oxidative Stress ◽  
Flow Cytometry ◽  
Confocal Microscopy ◽  
Vicia Faba ◽  
In Silico ◽  
Yeast Cells ◽  
Hypoglycemic Effect ◽  
Vicia Faba L ◽  
Faba Beans

The Nutritive Value of Ten Inbred Lines of Faba Beans (Vicia faba L.) in Relation to their Content of Antinutritional Constituents and Protein Quality

1988 ◽  
Vol 101 (4) ◽  
pp. 277-291 ◽  
Author(s):  
B. Bjerg ◽  
E. Kbmeyer ◽  
B. O. Eggum ◽  
T. Larsen ◽  
G. Robbelen ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Nutritive Value ◽  
Protein Quality ◽  
Inbred Lines ◽  
Vicia Faba L ◽  
Faba Beans

scholarly journals Overexpression of Mitochondrial Leishmania major Ascorbate Peroxidase Enhances Tolerance to Oxidative Stress-Induced Programmed Cell Death and Protein Damage

2009 ◽  
Vol 8 (11) ◽  
pp. 1721-1731 ◽  
Author(s):  
Subhankar Dolai ◽  
Rajesh K. Yadav ◽  
Swati Pal ◽  
Subrata Adak
Keyword(s):  
Oxidative Stress ◽  
Flow Cytometry ◽  
Confocal Microscopy ◽  
Ascorbate Peroxidase ◽  
Leishmania Major ◽  
Mitochondrial Respiratory Chain ◽  
Protein Carbonyl ◽  
Endonuclease G ◽  
Mitochondrial Membrane Depolarization ◽  
Induced Apoptosis

ABSTRACT Ascorbate peroxidase from Leishmania major (LmAPX) is one of the key enzymes for scavenging of reactive oxygen species generated from the mitochondrial respiratory chain. We have investigated whether mitochondrial LmAPX has any role in oxidative stress-induced apoptosis. The measurement of reduced glutathione (GSH) and protein carbonyl contents in cellular homogenates indicates that overexpression of LmAPX protects Leishmania cells against depletion of GSH and oxidative damage of proteins by H2O2 or camptothecin (CPT) treatment. Confocal microscopy and fluorescence spectroscopy data have revealed that the intracellular elevation of Ca2+ attained by the LmAPX-overexpressing cells was always below that attained in control cells. Flow cytometry assay data and confocal microscopy observation strongly suggest that LmAPX overexpression protects cells from H2O2-induced mitochondrial membrane depolarization as well as ATP decrease. Western blot data suggest that overexpression of LmAPX shields against H2O2- or CPT-induced cytochrome c and endonuclease G release from mitochondria and subsequently their accumulation in the cytoplasm. Caspase activity assay by flow cytometry shows a lower level of caspase-like protease activity in LmAPX-overexpressing cells under apoptotic stimuli. The data on phosphatidylserine exposed on the cell surface and DNA fragmentation results show that overexpression of LmAPX renders the Leishmania cells more resistant to apoptosis provoked by H2O2 or CPT treatment. Taken together, these results indicate that constitutive overexpression of LmAPX in the mitochondria of L. major prevents cells from the deleterious effects of oxidative stress, that is, mitochondrial dysfunction and cellular death.

Kinetic and thermodynamic compensation study of the hydration of faba beans (Vicia faba L.)

2019 ◽  
Vol 119 ◽  
pp. 390-397
Author(s):  
A. Garvín ◽  
P.E.D. Augusto ◽  
R. Ibarz ◽  
A. Ibarz
Keyword(s):  
Vicia Faba ◽  
Vicia Faba L ◽  
Faba Beans

Purification, Identification and Characterization of a Growth Inhibitor in Faba Beans (Vicia faba L. var. minor)

1977 ◽  
Vol 107 (7) ◽  
pp. 1313-1324 ◽  
Author(s):  
Ronald R. Marquardt ◽  
A. Thomas Ward ◽  
Lloyd D. Campbell ◽  
Peter E. Cansfield
Keyword(s):  
Vicia Faba ◽  
Growth Inhibitor ◽  
Vicia Faba L ◽  
Faba Beans ◽  
Identification And Characterization

NATURAL CROSS POLLINATION OF FABA BEANS (Vicia faba L.) GROWN IN MANITOBA

1984 ◽  
Vol 64 (1) ◽  
pp. 43-46 ◽  
Author(s):  
P. B. E. McVETTY ◽  
J. NUGENT-RIGBY
Keyword(s):  
Vicia Faba ◽  
Black Spot ◽  
Environmental Stresses ◽  
Cross Pollination ◽  
Year Effect ◽  
Vicia Faba L ◽  
Faba Beans ◽  
White Test ◽  
Natural Cross

Four populations of faba beans (Vicia faba L.) consisting of the black-spot-flowered varieties, Ackerperle, Diana, Herz Freya and UMFB-22, each combined with the white flowered variety, Triple White, were grown at three locations in each of 2 yr. The extent of natural cross pollination was estimated from a subsequent year’s grow-out of the Triple White test seed. The extent of natural cross pollination differed for varieties, locations and years, with years being the most significant. The year effect was probably due to different environmental stresses on the faba beans.Key words: Vicia faba, fertilization

pH, TEMPERATURE AND HYDRATION KINETICS OF FABA BEANS (VICIA FABA L.)

2003 ◽  
Vol 27 (1) ◽  
pp. 9-20 ◽  
Author(s):  
NANOR HALADJIAN ◽  
RIMA FAYAD ◽  
IMAD TOUFEILI ◽  
SOSSY SHADAREVIAN ◽  
MOATASIM SIDAHMED ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Hydration Kinetics ◽  
Vicia Faba L ◽  
Faba Beans ◽  
Kinetics Of
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