Adhesion of Pseudomonas aeruginosa, Achromobacter xylosoxidans, Delftia acidovorans, Stenotrophomonas maltophilia to contact lenses under the influence of an artificial tear solution

Biofouling ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 32-43 ◽  
Author(s):  
Jaya Dantam ◽  
Lakshman N. Subbaraman ◽  
Lyndon Jones
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stenotrophomonas Maltophilia ◽  
Contact Lenses ◽  
Achromobacter Xylosoxidans ◽  
Delftia Acidovorans ◽  
Artificial Tear

Comparative activity of antimicrobials against Pseudomonas aeruginosa, Achromobacter xylosoxidans and Stenotrophomonas maltophilia keratitis isolates

2018 ◽  
Vol 102 (5) ◽  
pp. 708-712 ◽  
Author(s):  
Oriel Spierer ◽  
Darlene Miller ◽  
Terrence P O’Brien
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stenotrophomonas Maltophilia ◽  
Antimicrobial Agents ◽  
Achromobacter Xylosoxidans ◽  
Eye Drops ◽  
Beta Lactams ◽  
Intrinsic Resistance ◽  
Significant Difference ◽  
Susceptibility Rate

Background/aimsAchromobacter xylosoxidans and Stenotrophomonas maltophilia are emerging corneal pathogens, which are closely related to Pseudomonas aeruginosa, and have intrinsic resistance to many commonly available antimicrobials. The purpose of this study is to compare the in vitro efficacy of 12 antimicrobial agents against A. xylosoxidans, S. maltophilia and P. aeruginosa isolates recovered from clinical cases of keratitis.MethodsRecovered corneal isolates (n=58) were identified and extracted from the Microbiology Data Bank of the Bascom Palmer Eye Institute. Comparative in vitro minimum inhibitory concentration (MIC) susceptibility profiles for fluoroquinolones, aminoglycosides, beta-lactams and miscellaneous antibiotics were recorded using the E-test methodology. Pharmacodynamic indices (Cmax/MIC) were calculated.ResultsA. xylosoxidans and S. maltophilia isolates were resistant to fluoroquinolones, aminoglycosides and ceftazidime (susceptibility rate ranging from 0% to 30%) while P. aeruginosa isolates showed a susceptibility rate of 95%–100% to these antimicrobials (P<0.00001 for the various antimicrobials). Exception was moxifloxacin with 80% of susceptibility rate to S. maltophilia isolates and Cmax/MIC=10.19. Ninety to 100% susceptibility rates were found for minocycline and trimethoprim/sulfamethoxazole for both A. xylosoxidans and S. maltophilia. One hundred per cent of the A. xylosoxidans isolates were susceptible to piperacillin/tazobactam and ticarcillin/clavulanic acid.ConclusionsThere is a significant difference in susceptibility patterns between A. xylosoxidans, S. maltophilia and P. aeruginosa. Fluoroquinolones and aminoglycosides may not be effective against A. xylosoxidans and S. maltophilia. Antibiotics that are not commercially available as eye drops, such as beta-lactams for A. xylosoxidans, and trimethoprim/sulfamethoxazole and minocycline for both A. xylosoxidans and S. maltophilia should be considered.

scholarly journals ISOLASI DAN KARAKTERISASI BAKTERI NITRIFIKASI DAN DENITRIFIKASI SEBAGAI KANDIDAT PROBIOTIK

2018 ◽  
Vol 12 (4) ◽  
pp. 369 ◽  
Author(s):  
Yosmaniar Yosmaniar ◽  
Hessy Novita ◽  
Eri Setiadi
Keyword(s):  
Water Quality ◽  
Pseudomonas Aeruginosa ◽  
Stenotrophomonas Maltophilia ◽  
Denitrifying Bacteria ◽  
Achromobacter Xylosoxidans ◽  
Nitrogen Compounds ◽  
Burkholderia Vietnamiensis ◽  
Ochrobactrum Intermedium

Senyawa nitrogen yang tinggi pada limbah budidaya perikanan intensif dapat memperburuk kualitas air, sehingga perlu diatasi dengan penambahan probiotik untuk proses bioremediasi. Tujuan penelitian ini adalah untuk mendapatkan bakteri nitrifikasi dan denitrifikasi yang berpotensi sebagai kandidat probiotik pengendali senyawa nitrogen pada budidaya ikan air tawar. Tahap penelitian terdiri atas: 1) koleksi sampel air dan sedimen dari kolam budidaya ikan patin di kawasan minapolitan Desa Pudak Kecamatan Kumpeh Kabupaten Muaro Jambi Provinsi Jambi dan Desa Koto Mesjid Kecamatan XIII Koto Kampar Kabupaten Kampar Provinsi Riau; 2) pengujian sampel secara in vitro yang meliputi: a) Isolasi dan seleksi bakteri nitrifikasi dan denitrifikasi; b) Karakterisasi morfologis bakteri terpilih; c) Karakterisasi fisiologi/biokimia isolat bakteri terpilih; d) Karakterisasi genetika isolat bakteri terpilih dengan sekuensing 16S-rRNA. Analisis data dilakukan secara deskriptif. Berdasarkan hasil penelitian diperoleh empat isolat bakteri nitrifikasi dan empat isolate bakteri denitrifikasi. Isolat bakteri nitrifikasi Pandoraea pnomenusa strain 1318 (NP1); Pseudomonas aeruginosa strain PSE12 (NP2); Pseudomonas aeruginosa strain PSE12 (NP3); Burkholderia vietnamiensis strain NE 7 (NP4); dan denitrifikasi Achromobacter xylosoxidans strain TPL14 (DP1); Stenotrophomonas acidaminiphila strain BTY (DP2); Stenotrophomonas maltophilia strain BHWSL2 (DP3); Ochrobactrum intermedium strain: SQ 20 (DP4) Achromobacter xylosoxidans strain TPL14 (DP1); Stenotrophomonas acidaminiphila strain BTY (DP2); Stenotrophomonas maltophilia strain BHWSL2 (DP3); Ochrobactrum intermedium strain: SQ 20 (DP4); yang berpotensi digunakan sebagai kandidat probiotik pengendali senyawa nitrogen pada budidaya ikan air tawar. Wastes from an intensive aquaculture contain nitrogen compounds which, if untreated, could rapidly reduce water quality condition within the system. The addition of probiotics as bioremediation to aquaculture system has been used to improve water quality with promising results. The aim of this study was to obtain potential nitrifying and denitrifying bacteria that could be used as probiotic candidates to control excessive nitrogen compounds in freshwater culture. This study consisted of two steps, 1) the collection of water samples and sediments from catfish ponds at ‘Minapolitan Area” in Pudak Village, Jambi Province and Koto Mesjid Village, Riau Province; 2) in vitro tests consisting of isolation and selection of nitrifying and denitrifying bacteria; morphological characterization of the selected nitrifying and denitrifying bacteria; characterization of physiological/biochemical selected nitrifying and denitrifying bacteria; genetic characterization of the selected nitrifying and denitrifying bacteria with 16SrRNA sequencing. All data were analyzed descriptively. The study had found four nitrifying bacteria isolates: Pandoraea pnomenusa strain 1318 (NP1); Pseudomonas aeruginosa strain PSE 12 (NP2); Pseudomonas aeruginosa strain PSE12 (NP3); Burkholderia vietnamiensis strain NE 7 (NP4). The study also found four isolates of denitrifying bacteria isolates: Achromobacter xylosoxidans strain TPL14 (DP1); Stenotrophomonas acidaminiphila strain BTY (DP2); Stenotrophomonas maltophilia strain BHWSL2 (DP3); Ochrobactrum intermedium strain: SQ 20 (DP4). All the identified nitrifying and denitrifying bacteria isolates have the potential to be used as probiotic candidates to control nitrogen compound in freshwater aquaculture.

scholarly journals Infekcijos etiologija sergant cistine fibroze

2011 ◽  
Vol 9 (2) ◽  
Author(s):  
Astra Vitkauskienė
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Stenotrophomonas Maltophilia ◽  
Achromobacter Xylosoxidans

Pirmoji bakterija, kurios klinikinė reikšmė infekcijos etiologijoje sergant cistine fibroze buvo lengvai nustatyta, yra Staphylococcus aureus. Pseudomonas aeruginosa (P. aeruginosa), kuri buvo dažnai išskiriama cistine fibroze sergantiems pacientams 1960 m., 1970 m. pradžioje laikyta ne patogenu ir iki 1990 m. dėl jos reikšmės buvo diskutuojama. Dabar P. aeruginosa klinikinė reikšmė įrodyta. Nustatyta, kad yra ir kitų, retesnių, sukėlėjų, kurie svarbūs cistine fibroze sergan-tiems pacientams: Stenotrophomonas maltophilia (anksčiau vadinta P. maltophilia, Xantomonas maltophilia), Achromobacter xylosoxidans, Pandoraea spp. (įskaitant Pandoraea apista), netuberkuliozinės Mycobacteria spp. Mikrobiologinė diagnostika ir rezultatų interpretavimas tiriant cistine fibroze sergančius pacientus turi tam tikrų savitumų. Interpretuojant rezultatus, reikia atskirti laikiną kolonizaciją nuo lėtinės infekcijos, dažnai atliekant kartotinius pasėlius ir/arba nustatant antikūnų atsaką į mikroorganizmą. Jei skrepliuose išskirtas sukėlėjas susijęs su klinikiniais uždegimo simptomais, patologiniais radio-loginiais požymiais, kvėpavimo funkcijos blogėjimu, pasėlyje šio sukėlėjo kiekiui mažėjant ir išnykus, gydant antibakteriniais vaistais, turi būti pastebimas ir klinikinis būklės gerėjimas. Kvalifikuota mikrobiologinė tarnyba, galinti tiksliai nustatyti patogeno morfologinį tipą ir padėti parinkti optimalius antibiotikus, yra būtina sėkmingo antimikrobinio cistinės fibrozės gydymo sąlyga.

Efficacy of Ophthalmic Solutions to Detach Adhering Pseudomonas aeruginosa from Contact Lenses

Cornea ◽  
1998 ◽  
Vol 17 (3) ◽  
pp. 293-300 ◽  
Author(s):  
Andrew S. Landa ◽  
Henny C. van der Mei ◽  
Gabriel van Rij ◽  
Henk J. Busscher
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contact Lenses ◽  
Ophthalmic Solutions

Diagnostic Value of Multiplex Polymerase Chain Reaction in Detection of Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from Sepsis in Pediatrics

2021 ◽  
Vol 15 ◽  
Author(s):  
Sara Galeb ◽  
Maysaa El Sayed Zaki ◽  
Raghdaa Shrief ◽  
Rasha Hassan ◽  
Mohamed Anies
Keyword(s):  
Polymerase Chain Reaction ◽  
Pseudomonas Aeruginosa ◽  
Acinetobacter Baumannii ◽  
Multiplex Pcr ◽  
Stenotrophomonas Maltophilia ◽  
Multiplex Polymerase Chain Reaction ◽  
Diagnostic Value ◽  
Blood Cultures ◽  
Chain Reaction ◽  
Polymerase Chain

Background: Proper identification of the causative organism in pediatric sepsis is crucial for early diagnosis and prevention of septic shock and organ failure. Objectives: The present study aimed to evaluate the multiplex Polymerase Chain Reaction (PCR) to detect Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from positive blood cultures for these pathogens isolated from children, with hospital-acquired sepsis compared to the conventional biochemical reactions for identification of these organisms. Methods: This study was a cross-sectional study performed on 100 isolates from pediatric blood cultures, including Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The study also included 100 isolates of Escherichia coli as a negative control. All isolates were identified by API 20NE and the multiplex PCR, with primers specific to the 3 tested bacteria. Results: Multiplex PCR was positive in 96% of isolates, and 4 isolates had negative results. False positive results were reported with three E. coli strains. Multiplex PCR identified all the isolates of Acinetobacter baumannii, 29 isolates of Pseudomonas aeruginosa, and 27 isolates of Stenotrophomonas maltophilia. Compared to the biochemical identification, the diagnostic value of the multiplex PCR revealed 96.04% sensitivity, 96.9% specificity, 97.00%, positive predictive value, 96.00% negative predictive value, and 96.50% accuracy. Conclusion: The present study highlights the diagnostic value of multiplex PCR to identify Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from positive blood cultures. Multiplex PCR was sensitive, specific, and accurate. The accuracy differs according to the organisms, with 100% accuracy for Acinetobacter baumannii.

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