Effect of potassium persulphate addition on sludge disintegration of a mesophilic anaerobic fermentation system

2020 ◽  
pp. 1-14
Author(s):  
Jinghua Lv ◽  
Mengmiao Tu ◽  
Xingyue Chen ◽  
Suzhou Li ◽  
Yunbei Li ◽  
...  
Keyword(s):  
Anaerobic Fermentation ◽  
Potassium Persulphate ◽  
Fermentation System

Study of Gas Production by Anaerobic Fermentation and Microbial Community Changes by In-Situ Microaerobic Desulphurization

2020 ◽  
Vol 14 (4) ◽  
pp. 517-523
Author(s):  
Yan Zhou ◽  
Hucheng Liu ◽  
Wei Kou ◽  
Lijie Shao ◽  
Peihan Liu ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
High Efficiency ◽  
Anaerobic Fermentation ◽  
Low Cost ◽  
Total Solid ◽  
Common Species ◽  
Gas Production ◽  
High Concentration ◽  
Fermentation System ◽  
The Stability

The enhancement of biogas quality at low cost and high efficiency process was one of the purposes of biogas engineering. In this work, we designed a reactor for microaerobic desulphurization. We used this reactor to study the anaerobic fermentation in systems that used cow manure with total solid (TS) concentrations of 18.5%, 15% and 10%. The influence of anaerobic fermentation on the stability of gas production and the characteristics of the gas produced with different concentrations of fermentation materials was studied. The strain structure of the fermentation system was obtained by high-throughput sequencing and taxonomy was compared. The H2S removal results showed that the average rates of the H2S removal in concentrations of fermentation materials of 18.5%, 15%, and 10% TS were 99.2%, 97.8%, and 78.8%, respectively. 16SrRNA sequencing was performed in different fermented samples as well as a comparison between samples in order to determine the number of unique species (NUS) and the number of common species (NCS). By comparing TS 18.5 with TS 15 and TS 10 samples, it was determined that under fermentation conditions, NUS were 113 and 106, respectively. Whereas NUS were 31 and 41, respectively, when comparing TS 15 and TS 10. These demonstrated that the number of strain species in the fermentation system with TS 18.5% was far more than those found in the systems with low concentration of fermentation. Also, the ability for disturbance resistance of the microaerobic desulphurization system was stronger at high concentration of the fermentation.

The New Hydrogenogen and its Industrial Application in Molasses Fermentation System

2011 ◽  
Vol 183-185 ◽  
pp. 542-546
Author(s):  
Jing Wei Zhang ◽  
Wei Han ◽  
Jian Yu Yang ◽  
Zhan Qing Wang ◽  
Yong Juan Zhang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Hydrogen Production ◽  
Production Technology ◽  
Anaerobic Fermentation ◽  
Industrial Applications ◽  
Hydrogen Metabolism ◽  
Product Analysis ◽  
Flat Panel ◽  
Fermentation System ◽  
Fermentation Liquid

The important premise for hydrogen production technology of fermentation generator was to separate and identify the efficient hydrogen producing bacteria. By use of Hungater technology and flat-panel technology with wide-body narrow-neck flask, LM-1 and HPB-LR medium to separate and identify the anaerobic fermentation hydrogen production bacteria, and five hydrogen-producing bacteria were obtained, whose hydrogen metabolism was ethanol fermentation. The product analysis on the hydrogen production bacteria fermentation liquid showed that ethanol and acetic acid accounted for 95%-99% of the total metabolites. These strains can be fermented to produce hydrogen, and can be used for industrial applications.

scholarly journals Effect of Zn Addition on the Cd-Containing Anaerobic Fermentation Process: Biodegradation and Microbial Communities

2019 ◽  
Vol 16 (16) ◽  
pp. 2998 ◽  
Author(s):  
Yonglan Tian ◽  
Huayong Zhang ◽  
Lei Zheng ◽  
Shusen Li ◽  
He Hao ◽  
...  
Keyword(s):  
Fermentation Process ◽  
Anaerobic Fermentation ◽  
Cost Effective ◽  
Gas Production ◽  
Process Stability ◽  
Absolute Abundance ◽  
Biomass Waste ◽  
Fermentation System ◽  
Zn Addition ◽  
Bacteria Communities

Anaerobic fermentation is considered as a cost-effective way of biomass waste disposal. However, the compound heavy metals contained in the biomass may induce complex effects on anaerobic fermentation, which limit the utilization of metal-contaminated biowaste. In this study, the impacts of Cd and Zn addition on biogas properties, process stability, substrate biodegradation, enzyme activity, and microbial properties were studied. The results showed that the addition of Cd together with Zn (Cd+Zn) increased the maximum daily and cumulative biogas yields, and brought forward the gas production peak compared with the Cd-added group. Taking the whole fermentation process into account, the promotion effects of adding Zn into the Cd-containing fermentation system on biogas yields were mainly attributable to better process stability, higher average NH4+-N concentration in the later stage of fermentation, reduced COD (p < 0.05), and increased biodegradability of lignocelluloses (p < 0.01), especially cellulose (p < 0.05) and lignin (p < 0.01). Meanwhile, the addition of Zn promoted the coenzyme M activity (p < 0.05), and increased the absolute abundance of Methanothermobacter. The bacteria communities during the fermentation process were responsible for the degradation of lignocelluloses. The results demonstrated that the addition of appropriate Zn into the Cd-containing fermentation system enhanced the efficiency of anaerobic fermentation and utilization of biowaste.

scholarly journals Paenibacillus ottowii sp. nov. isolated from a fermentation system processing bovine manure

2020 ◽  
Vol 70 (3) ◽  
pp. 1463-1469 ◽  
Author(s):  
Luis F. Velazquez ◽  
Shashi Rajbanshi ◽  
Shaohua Guan ◽  
Maud Hinchee ◽  
Allana Welsh
Keyword(s):  
Anaerobic Fermentation ◽  
Phylogenetic Analyses ◽  
Paenibacillus Polymyxa ◽  
Optimal Growth ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Diagnostic Features ◽  
Content Type ◽  
Fermentation System ◽  
Link Type

Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas. Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA–DNA relatedness through DNA–DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively. Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores. The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species. The G+C content of MS2379T is 45.9 mol%. Fermentation of glucose yields acid and gas end-products. The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine. The growth range of MS2379T was observed from 10–45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6–10 and up to 3 % NaCl. Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction. Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T. Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp. nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).

scholarly journals Determining functional fiber properties of berry pomaces via an anaerobic fermentation system

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Mfamara L Goita ◽  
Kathleen Lee McCutcheon ◽  
Anne M Raggio ◽  
John W Finley
Keyword(s):  
Anaerobic Fermentation ◽  
Fermentation System ◽  
Fiber Properties ◽  
Functional Fiber

Adaptability of a caproate-producing bacterium contributes to its dominance in an anaerobic fermentation system

2021 ◽  
Author(s):  
Huilin Wang ◽  
Yang Gu ◽  
Weicheng Zhou ◽  
Dong Zhao ◽  
Zongwei Qiao ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Fermentation ◽  
Abiotic Factors ◽  
Microbial Community Composition ◽  
Mixed Cultures ◽  
Ph Homeostasis ◽  
Fermentation System ◽  
Medium Chain ◽  
Metabolic Versatility ◽  
Pit Mud

The transformation of diverse feedstocks into medium-chain fatty acids (MCFAs) by mixed cultures is a promising biorefinery route because of the high value of MCFAs. A particular concern is how to maintain the microbial consortia in mixed cultures to achieve stable MCFA production. Chinese strong aroma-type liquor ( Baijiu ) fermentation system continually produces caproic acid for decades through a spontaneous inoculation of anaerobes from pit mud into fermented grains. Therefore, illuminating the dominant caproate-producing bacterium (CPB) in pit mud and how the CPB sustains in the spontaneous fermentation system will benefit to reveal the microbiological mechanisms of the stable caproate production. Here, we examined pit mud samples across four Chinese strong aroma-type Baijiu producing areas and found that a caproate-producing Caproicibacterium sp. was widely distributed in these distilleries with relative abundance ranging from 1.4% to 35.5% and an average abundance of 11.4%. Through controlling carbon source availability, we achieved different simplified caproate-producing consortia and found that the growth advantage of Caproicibacterium sp. was highly dependent on glucose. Then two strains, named Caproicibacterium sp. LBM19010 and Caproicibacterium sp. JNU-WLY1368, were isolated from pit mud of two regions. The metabolic versatility of this bacterium utilizing starch, maltose, glucose and lactate reflected its adaptability to the fermentation environment where these carbon sources coexist. The simultaneous utilization of glucose and lactate contributed to the balance between cell growth and pH homeostasis. This study reveals that multiple adaptation strategies employed by the predominant CPB promotes its stability and dominance in a saccharide- and lactate-rich anaerobic habitat. IMPORTANCE Chinese strong aroma-type liquor ( Baijiu ) fermentation environment is a typical medium-chain fatty acid producing system with complex nutrients. Although several studies have revealed the correlation between microbial community composition and abiotic factors, the adaptation mechanisms of dominant species to abiotic environment are still unknown in this special anaerobic habitat. This study identified the predominant CPB in Chinese strong aroma-type Baijiu fermentation system. Metabolic versatility and flexibility of the dominant CPB with a small-size genome indicated that this bacterium can effectively exploit available carbon and nitrogen sources, which could be a key factor to promote its ecological success in a multi-species environment. The understanding of growth and metabolic features of CPB responsible for its dominance in microbial community will not only contribute to the improvement of Chinese strong aroma-type Baijiu production but also expand its potential industrial applications in caproate production.

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