scholarly journals Exhaled nitric oxide in mylar balloons: influence of storage time, humidity and temperature

2003 ◽  
Vol 12 (1) ◽  
pp. 47-49 ◽  
Author(s):  
Alessandro Bodini ◽  
Mariëlle W. H. Pijnenburg ◽  
Atillio L. Boner ◽  
Johan C. de Jongste
Keyword(s):  
Nitric Oxide ◽  
Silica Gel ◽  
Storage Time ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Air Samples ◽  
Exhaled Air ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration ◽  
The Stability

Background:Mylar balloons are used to collect exhaled air for analysis of fractional nitric oxide concentration (FENO).Aim:We studied the effect of storage conditions on the stability of nitric oxide (NO) in mylar balloons.Methods:Exhaled air samples and calibration gases were stored in mylar balloons at 4, 21 and 37°C, with or without silica gel. NO was measured after 0, 6, 9, 24 and 48 h. Scheffe F-tests were used to compare NO values. Results NO remained stable in balloons for 9 h at all temperatures, without silica gel. NO increased between 9 and 48 h, but only with low initial FENO. Silica gel increased variability.Conclusions:FENO in mylar balloons is stable for at least 9 h. The storage temperature is not critical, but silica gel increases variability.

scholarly journals Influence of Storage Conditions and Preservatives on Metabolite Fingerprints in Urine

Metabolites ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 203 ◽  
Author(s):  
Xinchen Wang ◽  
Haiwei Gu ◽  
Susana A. Palma-Duran ◽  
Andres Fierro ◽  
Paniz Jasbi ◽  
...  
Keyword(s):  
Large Scale ◽  
Storage Time ◽  
Storage Temperature ◽  
Urinary Metabolites ◽  
Storage Conditions ◽  
Inhibitory Effect ◽  
Urine Samples ◽  
C Storage ◽  
Liquid Chromatography Tandem Mass ◽  
The Stability

Human urine, which is rich in metabolites, provides valuable approaches for biomarker measurement. Maintaining the stability of metabolites in urine is critical for accurate and reliable research results and subsequent interpretation. In this study, the effect of storage temperature (4, 22, and 40 °C), storage time (24 and 48 h), and use of preservatives (boric acid (BA), thymol) and para-aminobenzoic acid (PABA) on urinary metabolites in the pooled urine samples from 20 participants was systematically investigated using large-scale targeted liquid chromatography tandem mass spectrometry (LC-MS/MS)-based metabolomics. Statistical analysis of 158 reliably detected metabolites showed that metabolites in urine with no preservative remained stable at 4 °C for 24 and 48 h as well as at 22 °C for 24 h, but significant metabolite differences were observed in urine stored at 22 °C for 48 h and at 40 °C. The mere addition of BA caused metabolite changes. Thymol was observed to be effective in maintaining metabolite stability in urine in all the conditions designed, most likely due to the inhibitory effect of thymol on urine microbiota. Our results provide valuable urine preservation guidance during sample storage, which is essential for obtaining reliable, accurate, and reproducible analytical results from urine samples.

scholarly journals Influence of atmospheric nitric oxide concentration on the measurement of nitric oxide in exhaled air

Thorax ◽  
1998 ◽  
Vol 53 (8) ◽  
pp. 673-676 ◽  
Author(s):  
M. Corradi ◽  
A. Pelizzoni ◽  
M. Majori ◽  
A. Cuomo ◽  
E. d. Munari ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Exhaled Air ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration

Relationship between alveolar nitric oxide concentration in exhaled air and small airway function in COPD

2013 ◽  
Vol 7 (4) ◽  
pp. 046002 ◽  
Author(s):  
Tsunahiko Hirano ◽  
Kazuto Matsunaga ◽  
Hisatoshi Sugiura ◽  
Yoshiaki Minakata ◽  
Akira Koarai ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Small Airway ◽  
Airway Function ◽  
Exhaled Air ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration

scholarly journals The effect of silica desiccation under different storage conditions on filter-immobilized environmental DNA

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Michael J. Allison ◽  
Jessica M. Round ◽  
Lauren C. Bergman ◽  
Ali Mirabzadeh ◽  
Heather Allen ◽  
...  
Keyword(s):  
Silica Gel ◽  
Storage Temperature ◽  
Environmental Dna ◽  
Cost Effective ◽  
Storage Conditions ◽  
Systematic Evaluation ◽  
Silica Bead ◽  
Gel Beads ◽  
Polymerase Chain

Abstract Objective Silica gel beads have promise as a non-toxic, cost-effective, portable method for storing environmental DNA (eDNA) immobilized on filter membranes. Consequently, many ecological surveys are turning to silica bead filter desiccation rather than ethanol preservation. However, no systematic evaluation of silica bead storage conditions or duration past 1 week has been published. The present study evaluates the quality of filter-immobilized eDNA desiccated with silica gel under different storage conditions for over a year using targeted quantitative real-time polymerase chain reaction (qPCR)-based assays. Results While the detection of relatively abundant eDNA target was stable over 15 months from either ethanol- or silica gel-preserved filters at − 20 and 4 °C, silica gel out-performed ethanol preservation at 23 °C by preventing a progressive decrease in eDNA sample quality. Silica gel filter desiccation preserved low abundance eDNA equally well up to 1 month regardless of storage temperature (18, 4, or − 20 °C). However only storage at − 20 °C prevented a noticeable decrease in detectability at 5 and 12 months. The results indicate that brief storage of eDNA filters with silica gel beads up to 1 month can be successfully accomplished at a range of temperatures. However, longer-term storage should be at − 20 °C to maximize sample integrity.

Effect of round window membrane application of nitric oxide on hearing and nitric oxide concentration in perilymph

2003 ◽  
Vol 67 (6) ◽  
pp. 585-590 ◽  
Author(s):  
Jonathan B. Hanson ◽  
Paul T. Russell ◽  
Andy T.A. Chung ◽  
Claire S. Kaura ◽  
Samantha H. Kaura ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Round Window ◽  
Round Window Membrane ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration
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