A 67-bp variable duplication in the promoter region of the ADIPOQ is associated with milk traits in Xinjiang brown cattle

2021 ◽  
pp. 1-9
Author(s):  
Tingting Liu ◽  
Xing Ju ◽  
Menghua Zhang ◽  
Chen Wei ◽  
Dan Wang ◽  
...  
Keyword(s):  
Promoter Region ◽  
Milk Traits ◽  
Brown Cattle

The effect of single nucleotide polymorphism in the promoter region of bovine alpha-lactalbumin (LALBA) gene on LALBA expression in milk cells and milk traits of cows

2021 ◽  
Author(s):  
Malgorzata Ostrowska ◽  
Lech Zwierzchowski ◽  
Paulina Brzozowska ◽  
Ewelina Kawecka-Grochocka ◽  
Beata Żelazowska ◽  
...  
Keyword(s):  
Promoter Region ◽  
Somatic Cells ◽  
Production Traits ◽  
Milk Traits ◽  
Single Nucleotide ◽  
Protein Levels ◽  
Holstein Friesian ◽  
Daily Milk Yield ◽  
Retinoid X Receptor Alpha ◽  
Alpha Lactalbumin

Abstract Polymorphisms of milk protein genes have been proposed as candidate markers for dairy production traits in cattle. In the present study, a polymorphism was detected in the 5’flanking (promoter) region of the bovine alpha-lactalbumin (LALBA) gene, a T/C transition located at nucleotide (nt) -1001 relative to the transcription start site g.-1001T > C (NC_037332.1:g.31183170T > C) which is recognizable with PstI restriction endonuclease. In silico analyses showed that this mutation created novel retinoid X receptor alpha (RXRA) and vitamin D receptor (VDR) transcription factor binding sites. Real-time PCR found that cows with different genetic variants of the promoter demonstrated different levels of expression of LALBA mRNA in milk somatic cells (MSC). The TT genotype cows demonstrated low expression, while those with CT demonstrated much higher expression (P < 0.05). ELISA analysis found milk LALBA protein levels also differed between the TT and CT cows (P < 0.05), and that these levels were not correlated with the mRNA abundance in MSC. Association analysis found that the g.-1001T > C polymorphism in the promoter region of the LALBA gene influenced milk production traits in Polish Holstein-Friesian (HF) cows. High daily milk yield and dry matter yield, and high lactose yield and concentration were associated with the TT genotype. The TT genotype cows also had a lower number of somatic cells in the milk, considered as an indicator of udder health status. Therefore, the TT genotype could be more desirable from the breeder’s perspective.

Effects of eight InDel variants in FHIT on milk traits in Xinjiang brown cattle

2020 ◽  
pp. 1-9 ◽  
Author(s):  
Xing Ju ◽  
Xixia Huang ◽  
Menghua Zhang ◽  
Xianyong Lan ◽  
Dan Wang ◽  
...  
Keyword(s):  
Milk Traits ◽  
Brown Cattle

scholarly journals Determination of genetic variation within the <i>DYRK2</i> gene and its associations with milk traits in cattle

2020 ◽  
Vol 63 (2) ◽  
pp. 315-323
Author(s):  
Cui Mao ◽  
Xing Ju ◽  
Haijian Cheng ◽  
Xixia Huang ◽  
Fugui Jiang ◽  
...  
Keyword(s):  
Genome Wide Association Study ◽  
Cattle Breeding ◽  
Milk Traits ◽  
Dual Specificity ◽  
Genome Wide ◽  
Brown Cattle ◽  
Speed Up ◽  
Indel Variation ◽  
Kinesin Family

Abstract. To speed up the progress of marker-assisted selection (MAS) in cattle breeding, the dual-specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2), cadherin 2 (CDH2), and kinesin family member 1A (KIF1A) genes were chosen based on our pervious genome-wide association study (GWAS) analysis results. DYRK2 is a kinase that may participate in cell growth and/or development; it shows phosphorylation activity toward serine, threonine, and tyrosine fragments of proteins, and it is different from other protein kinases. The CDH2 gene encodes a classic cadherin, which is a member of the cadherin superfamily. The protein encoded by KIF1A is a member of the kinesin family and plays a role in the transportation of membrane organelles along axon microtubules. We detected insertion/deletion (InDel) variation in these three candidate genes in 438 individual cattle (Xinjiang Brown cattle and Wagyu × Luxi crossbreed cattle). Only DYRK2-P3-11 bp was polymorphic and genotyped. The polymorphism information content of DYRK2-P3-11 bp was 0.336. Correlation analyses showed that InDel polymorphism was significantly associated with six different milk traits. These findings may aid future analyses of InDel genotypes in cattle breeds, and speed up the progress of MAS in cattle breeding.

968: Gelsolin Gene Silencing Involving Unusual Chromatin Structures and a Novel Stem Loop Structure of the Promoter Region in Human Bladder Cancer

2004 ◽  
Vol 171 (4S) ◽  
pp. 256-257
Author(s):  
Kazunori Haga ◽  
Ataru Sazawa ◽  
Toru Harabayashi ◽  
Nobuo Shinohara ◽  
Minoru Nomoto ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Gene Silencing ◽  
Promoter Region ◽  
Loop Structure ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Stem Loop ◽  
Stem Loop Structure

Variation in the Promoter Region of the β Fibrinogen Gene Is Associated with Plasma Fibrinogen Levels in Smokers and Non-Smokers

1991 ◽  
Vol 65 (05) ◽  
pp. 487-490 ◽  
Author(s):  
A E Thomas ◽  
F R Green ◽  
C H Kelleher ◽  
H C Wilkes ◽  
P J Brennan ◽  
...  
Keyword(s):  
Heart Disease ◽  
Promoter Region ◽  
Fragment Length Polymorphism ◽  
Fibrinogen Level ◽  
Plasma Fibrinogen ◽  
Plasma Fibrinogen Level ◽  
Healthy Men ◽  
History Of ◽  
General Practices ◽  
Restriction Fragment Length

SummaryWe investigated the association between fibrinogen levels and a HaeIII restriction fragment length polymorphism located at −453 bp from the start of transcription of the β fibrinogen gene. 292 healthy men aged 45 to 69 years, recruited from general practices throughout Britain, were studied. None had a history of ischaemic heart disease. 41.1% (120) were smokers and fibrinogen levels were higher in this group. The frequency of the noncutting allele (designated H2) was 0.19 and was the same in smokers and non-smokers. The H2 allele was associated with elevated levels of fibrinogen in both smokers and non-smokers and the effect of genotype was similar in both groups. After smoking, HaeIII genotype was the strongest predictor of fibrinogen levels and explained 3.1% of the variance in fibrinogen levels. These results confirm earlier studies that variation at the fibrinogen locus contributes to the between-individual differences in plasma fibrinogen level.

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