scholarly journals Air-Liquid Interface Culture of Serially Passaged Human Nasal Epithelial Cell Monolayer forIn VitroDrug Transport Studies

Drug Delivery ◽  
2005 ◽  
Vol 12 (5) ◽  
pp. 305-311 ◽  
Author(s):  
Min-Ki Lee ◽  
Jin-Wook Yoo ◽  
Hongxia Lin ◽  
You-Sun Kim ◽  
Dae-Duk Kim ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Monolayer ◽  
Liquid Interface ◽  
Nasal Epithelial Cell ◽  
Human Nasal Epithelial Cell ◽  
Transport Studies ◽  
Air Liquid Interface

Enhancing effect of surfactants on fexofenadine·HCl transport across the human nasal epithelial cell monolayer

2007 ◽  
Vol 330 (1-2) ◽  
pp. 23-31 ◽  
Author(s):  
Hongxia Lin ◽  
Matthias Gebhardt ◽  
Shengjie Bian ◽  
Kyoung Ae Kwon ◽  
Chang-Koo Shim ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Monolayer ◽  
Nasal Epithelial Cell ◽  
Human Nasal Epithelial Cell ◽  
Enhancing Effect

Transport of anti-allergic drugs across the passage cultured human nasal epithelial cell monolayer

2005 ◽  
Vol 26 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Hongxia Lin ◽  
Jin-Wook Yoo ◽  
Hwan-Jung Roh ◽  
Min-Ki Lee ◽  
Suk-Jae Chung ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Monolayer ◽  
Nasal Epithelial Cell ◽  
Human Nasal Epithelial Cell

scholarly journals Enhancing Effect of Borneol and Muscone on Geniposide Transport across the Human Nasal Epithelial Cell Monolayer

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e101414 ◽  
Author(s):  
Zhenzhen Chen ◽  
Xin Gong ◽  
Yang Lu ◽  
Shouying Du ◽  
Zhihui Yang ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Monolayer ◽  
Nasal Epithelial Cell ◽  
Human Nasal Epithelial Cell ◽  
Enhancing Effect

Passive siRNA transfection method for gene knockdown in air-liquid interface airway epithelial cell cultures

2021 ◽  
Author(s):  
Colleen M Bartman ◽  
Kimberly E Stelzig ◽  
David R Linden ◽  
Y. S. Prakash ◽  
Sergio E Chiarella
Keyword(s):  
Epithelial Cell ◽  
Liquid Interface ◽  
Airway Epithelial ◽  
Loss Of Function ◽  
Sirna Transfection ◽  
Simple Method ◽  
Primary Epithelial Cell ◽  
Transfection Protocol ◽  
Air Liquid Interface

Differentiation of human bronchial epithelial cells (HBEs) in air-liquid interface (ALI) cultures recapitulates organotypic modeling of the in vivo environment. Although ALI cultures are invaluable for studying the respiratory epithelial barrier, loss-of-function studies are limited by potentially cytotoxic reagents in classical transfection methods, the length of the differentiation protocol, and the number of primary epithelial cell passages. Here, we present the efficacy and utility of a simple method for siRNA transfection of HBEs in ALI cultures that does not require potentially cytotoxic transfection reagents and does not detrimentally alter the physiology of HBEs during the differentiation process. This transfection protocol introduces a reproducible and efficient method for loss-of-function studies in HBE ALI cultures that can be leveraged for modeling the respiratory system and airway diseases.

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