Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using 99mTc-sulfur colloid functional imaging

2021 ◽  
pp. 1-7
Author(s):  
Fatma J. Al-Saeedi ◽  
Salah Kh. Al-Waheeb ◽  
Peramaiyan Rajendran ◽  
Khalid M. Khan ◽  
Moudhi Sadan
Keyword(s):  
Functional Imaging ◽  
Diabetic Rats ◽  
Early Initiation ◽  
Sulfur Colloid ◽  
Functional Changes ◽  
99Mtc Sulfur Colloid

Functional changes in adenylyl cyclases and associated decreases in relaxation responses in mesenteric arteries from diabetic rats

2005 ◽  
Vol 289 (5) ◽  
pp. H2234-H2243 ◽  
Author(s):  
Takayuki Matsumoto ◽  
Kentaro Wakabayashi ◽  
Tsuneo Kobayashi ◽  
Katsuo Kamata
Keyword(s):  
Mesenteric Artery ◽  
Diabetic Rats ◽  
Functional Change ◽  
Water Soluble ◽  
Mesenteric Arteries ◽  
Diabetic Rat ◽  
Adenylyl Cyclases ◽  
Camp Production ◽  
Functional Changes ◽  
Rat Mesenteric Artery

To assess the functional change in adenylyl cyclases (AC) associated with the diabetic state, we investigated AC-mediated relaxations and cAMP production in mesenteric arteries from rats with streptozotocin (STZ)-induced diabetes. The relaxations induced by the water-soluble forskolin (FSK) analog NKH477, which is a putative AC5 activator, but not by the β-adrenoceptor agonist isoproterenol (Iso) and the AC activator FSK, were reduced in intact diabetic mesenteric artery. In diabetic rats, however, Iso-, FSK-, and NKH477-induced relaxations were attenuated in the presence of inhibitors of nitric oxide synthase and cyclooxygenase. To exclude the influence of phosphodiesterase (PDE), we also examined the relaxations induced by several AC activators in the presence of 3-isobutyl-1-methylxanthine (IBMX; a PDE inhibitor). Under these conditions, the relaxation induced by Iso was greatly impaired in STZ-diabetic rats. This Iso-induced relaxation was significantly attenuated by pretreatment with SQ-22536, an AC inhibitor, in mesenteric rings from age-matched controls but not in those from STZ-diabetic rats. Under the same conditions, the relaxations induced by FSK or NKH477 were impaired in STZ-diabetic rats. Neither FSK- nor A-23187 (a Ca2+ ionophore)-induced cAMP production was significantly different between diabetics and controls. However, cAMP production induced by Iso or NKH477 was significantly impaired in diabetic mesenteric arteries. Expression of mRNAs and proteins for AC5/6 was lower in diabetic mesenteric arteries than in controls. These results suggest that AC-mediated relaxation is impaired in the STZ-diabetic rat mesenteric artery, perhaps reflecting a reduction in AC5/6 activity.

scholarly journals Retinal functional imager (RFI): Non-invasive functional imaging of the retina

2013 ◽  
Vol 5 (2) ◽  
pp. 250-257 ◽  
Author(s):  
Sunil Ganekal
Keyword(s):  
Functional Imaging ◽  
Structural Changes ◽  
Imaging System ◽  
Retinal Blood Flow ◽  
Capillary Perfusion ◽  
Functional Changes ◽  
Non Invasive ◽  
Retinal Disorders ◽  
Retinal Blood Flow Velocity ◽  
Perfusion Maps

Retinal functional imager (RFI) is a unique non-invasive functional imaging system with novel capabilities for visualizing the retina. The objective of this review was to show the utility of non-invasive functional imaging in various disorders. Electronic literature search was carried out using the websites www.pubmed.gov and www.google.com. The search words were retinal functional imager and non-invasive retinal imaging used in combination. The articles published or translated into English were studied. The RFI directly measures hemodynamic parameters such as retinal blood-flow velocity, oximetric state, metabolic responses to photic activation and generates capillary perfusion maps (CPM) that provides retinal vasculature detail similar to flourescein angiography. All of these parameters stand in a direct relationship to the function and therefore the health of the retina, and are known to be degraded in the course of retinal diseases. Detecting changes in retinal function aid early diagnosis and treatment as functional changes often precede structural changes in many retinal disorders. Nepal J Ophthalmol 2013; 5(10): 250-257 DOI: http://dx.doi.org/10.3126/nepjoph.v5i2.8738

scholarly journals Hypersensitivity of Spinothalamic Tract Neurons Associated With Diabetic Neuropathic Pain in Rats

2002 ◽  
Vol 87 (6) ◽  
pp. 2726-2733 ◽  
Author(s):  
Shao-Rui Chen ◽  
Hui-Lin Pan
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Thermal Hyperalgesia ◽  
Diabetic Rats ◽  
Projection Neurons ◽  
Diabetic Neuropathic Pain ◽  
Spinothalamic Tract ◽  
Functional Changes ◽  
Spinal Dorsal

Diabetic neuropathic pain is often considered to be caused by peripheral neuropathy. The involvement of the CNS in this pathological condition has not been well documented. Development of hypersensitivity of spinal dorsal horn neurons is involved in neuropathic pain induced by traumatic nerve injury. In the present study, we determined the functional changes of identified spinothalamic tract (STT) neurons and their correlation to diabetic neuropathic pain. Diabetes was induced in rats by intraperitoneal injection of streptozotocin. Hyperalgesia and allodynia were assessed by the withdrawal responses to pressure, radiant heat, and von Frey filaments applied to the hindpaw. Single-unit activity of STT neurons was recorded from the lumbar spinal cord in anesthetized rats. The responses of STT neurons to mechanical and thermal stimuli and the sensitivity to intravenous morphine were determined in diabetic and normal rats. In 12 diabetic rats, mechanical allodynia and hyperalgesia, but not thermal hyperalgesia, developed within 2 wk after streptozotocin injection and lasted for ≥7 wk. Compared to the 32 STT neurons recorded in normal animals, the 37 STT neurons in diabetic rats displayed a higher spontaneous discharge activity and enlarged receptive fields. Also, the STT neurons in diabetic rats exhibited lower thresholds and augmented responses to mechanical stimulation. Intravenous injection of 2.5 mg/kg of morphine suppressed significantly the responses of STT neurons to noxious stimuli in 12 nondiabetic rats. However, such an inhibitory effect of morphine on the evoked response of STT neurons was diminished in 14 diabetic animals. This electrophysiological study provides new information that development of hypersensitivity of spinal dorsal horn projection neurons may be closely related to neuropathic pain symptoms caused by diabetes. Furthermore, the attenuated inhibitory effects of morphine on evoked responses of STT neurons in diabetes likely accounts for its reduced analgesic efficacy in this clinical form of neuropathic pain.

scholarly journals Geometry-dependent functional changes in iPSC-derived cardiomyocytes probed by functional imaging and RNA sequencing

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0172671 ◽  
Author(s):  
Christopher A. Werley ◽  
Miao-Ping Chien ◽  
Jellert Gaublomme ◽  
Karthik Shekhar ◽  
Vincent Butty ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Functional Imaging ◽  
Functional Changes

scholarly journals Prognostic Utility of 90Y Radioembolization Dosimetry Based on Fusion 99mTc-Macroaggregated Albumin-99mTc-Sulfur Colloid SPECT

2013 ◽  
Vol 54 (12) ◽  
pp. 2055-2061 ◽  
Author(s):  
M. G. E. H. Lam ◽  
M. L. Goris ◽  
A. H. Iagaru ◽  
E. S. Mittra ◽  
J. D. Louie ◽  
...  
Keyword(s):  
Sulfur Colloid ◽  
Macroaggregated Albumin ◽  
Prognostic Utility ◽  
90Y Radioembolization ◽  
99Mtc Sulfur Colloid

scholarly journals Long-term Effects of Local Irradiation of the Marrow on Erythron and Red Cell Function

Blood ◽  
1970 ◽  
Vol 36 (5) ◽  
pp. 617-622 ◽  
Author(s):  
WIL B. NELP ◽  
MAHENDRA N. GOHIL ◽  
STEVEN M. LARSON ◽  
RAE ELLEN BOWER
Keyword(s):  
Cell Function ◽  
Cell Activity ◽  
Maximum Level ◽  
Similar Degree ◽  
Partial Recovery ◽  
Long Term Effects ◽  
Sulfur Colloid ◽  
Secondary Recovery ◽  
99Mtc Sulfur Colloid

Abstract Changes in erythron and RE cell function were examined in the marrow of the rabbit after 250-5000 R of localized irradiation by comparing the amount of 59Fe and 99mTc sulfur colloid concentrated in the irradiated tibiofibula to that in the unirradiated control. At all levels of irradiation, there was immediate and severe loss of erythron function while RE cell activity remained nearly intact. Erythron function showed prompt partial recovery to a maximum level at approximately 8 days but with doses greater than 1000 R there was a secondary decline to 20 per cent of normal during the next 8 weeks. After 15 days, RE cell function had decreased to the same level as the erythron and subsequently fell in parallel with it. After 15 months, the marrow showed a secondary recovery of both RE cell and erythron function to 50 and 66 per cent of normal. The results of these experiments suggest that radiocolloid photoscans of the marrow showing decreased or absent RE cell activity will reflect a similar degree of erythropoietic damage if the studies are obtained weeks or months following radiation therapy.

Age-related changes in endothelium-dependent relaxation in aorta from genetically diabetic WBN/Kob rats

1992 ◽  
Vol 262 (4) ◽  
pp. H1104-H1109 ◽  
Author(s):  
N. Miyata ◽  
K. Tsuchida ◽  
S. Okuyama ◽  
S. Otomo ◽  
K. Kamata ◽  
...  
Keyword(s):  
Sodium Nitroprusside ◽  
Matched Control ◽  
Vascular Complications ◽  
Diabetic Rats ◽  
Response Curves ◽  
Functional Changes ◽  
Age Related ◽  
Relaxation Responses ◽  
Effects Of Aging ◽  
Endothelium Dependent Relaxation

Experiments were designed to investigate the effects of aging and hyperglycemia on relaxation of the aorta for both endothelium-dependent and -independent agents in Wistar (control) and WBN/Kob (genetically diabetic) rats. The concentration of glucose in serum was elevated significantly in aged (90-92 wk) but not young (13-15 wk) WBN/Kob rats. Endothelium-dependent relaxations of both control and WBN/Kob rats to acetylcholine were reduced by aging. The relaxations induced by acetylcholine in aortic strips were significantly attenuated in both young (nondiabetic) and aged (diabetic) WBN/Kob rats, compared with those from age-matched control vessels, respectively. The concentration-response curves for sodium nitroprusside in aortic strips from both aged control and aged WBN/Kob rats were shifted to the left when compared with those from young rats, respectively. However, the maximal relaxation responses to sodium nitroprusside showed no difference among all vessels studied. The relaxations induced by sodium nitroprusside in aortic strips from both young and aged WBN/Kob rats were similar to those from age-matched control rats, respectively. The relaxations induced by atrial natriuretic peptide showed no difference among all vessels studied. In genetically diabetic rats, functional changes in endothelium occurred before elevation of the levels of glucose in the serum. Thus impaired endothelium-dependent relaxation may play an important role in the high incidence of vascular complications in diabetes mellitus.

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