Enhanced β-mannanase production by Bacillus licheniformis by optimizing carbon source and feeding regimes

2021 ◽  
pp. 1-9
Author(s):  
Yangcun Sun ◽  
Xiaohang Zhou ◽  
Wen Zhang ◽  
Xue Tian ◽  
Wenxiang Ping ◽  
...  
Keyword(s):  
Carbon Source ◽  
Bacillus Licheniformis ◽  
Feeding Regimes

scholarly journals Production of thermostable extracellular α-amylase by a moderate thermophilic Bacillus licheniformis isolated from Qinarje Hot Spring (Ardebil prov. of Iran)

2017 ◽  
Vol 118 (4) ◽  
Author(s):  
Ali Deljou ◽  
Iman Arezi
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Bacillus Licheniformis ◽  
Bacterial Growth ◽  
Enzyme Production ◽  
Hot Spring ◽  
Basal Medium ◽  
Thermophilic Bacterium ◽  
Inoculum Size ◽  
Amylase Production

Background and Purpose: Amylases are most important industrial enzymes that account for about 30% of the world’s food, feed, fermentation, textile, detergent and cellulosic industries. This study aimed at optimum production of thermostable α-amylase via moderate thermophilic bacterium (Bacillus licheniformis) which was recently isolated from Qinarje Hot spring.Material and Methods: Initially, ability of bacterium for amylase activity was determined by starch hydrolysis test using Gram’s iodine staining. Then bacterial growth pattern and amylase production curves in basal production medium were graphically determined at different time intervals. Finally, effect of different temperature, pH, carbon source, nitrogen source, minerals and inoculum size were studied on bacterial growth and amylase production using turbidimetric and DNS method, respectively.Results: Optimum enzyme production achieved after 84 hours of inoculation from cultures growing at 40 ˚C and pH 9.0 in a medium containing 0.03% (w/v) of CaCl2, compared to the basal medium, results showed that the best enzyme production happened with inoculum size of 4% (v/v). The addition of 1% (w/v) rice husk (as a Carbon source) enhanced enzyme productivity up to 160% and substitution of the peptone and yeast extract with 1% (w/v) of tryptone (as a Nitrogen source) increased the α-amylase production up to 160%.Conclusion: Our findings show that B. licheniformis-AZ2 strain has an ability to produce the thermostable α-amylase which is suitable in starch processing and food industries. To be commercialized, further investigation is required for enhancement of the enzyme production.Keywords: Bacillus licheniformis; Optimization; Basal medium; Agricultural by-products.

scholarly journals Biodegradation of malathion by Bacillus licheniformis strain ML-1

2016 ◽  
Vol 68 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Sara Khan ◽  
Habiba Zaffar ◽  
Usman Irshad ◽  
Raza Ahmad ◽  
Abdul Khan ◽  
...  
Keyword(s):  
Carbon Source ◽  
Bacillus Licheniformis ◽  
Sole Carbon Source ◽  
Sole Source ◽  
Minimal Salt Medium ◽  
Organophosphate Pesticide ◽  
Soil Enrichment ◽  
Enrichment Technique ◽  
Malathion Carboxylesterase

Malathion, a well-known organophosphate pesticide, has been used in agriculture over the last two decades for controlling pests of economically important crops. In the present study, a single bacterium, ML-1, was isolated by soil-enrichment technique and identified as Bacillus licheniformis on the basis of the 16S rRNA technique. The bacterium was grown in carbon-free minimal salt medium (MSM) and was found to be very efficient in utilizing malathion as the sole source of carbon. Biodegradation experiments were performed in MSM without carbon source to determine the malathion degradation by the selected strain, and the residues of malathion were determined quantitatively using HPLC techniques. Bacillus licheniformis showed very promising results and efficiently consumed malathion as the sole carbon source via malathion carboxylesterase (MCE), and about 78% malathion was degraded within 5 days. The carboxylesterase activity was determined by using crude extract while using malathion as substrate, and the residues were determined by HPLC. It has been found that the MCE hydrolyzed 87% malathion within 96 h of incubation. Characterization of crude MCE revealed that the enzyme is robust in nature in terms of organic solvents, as it was found to be stable in various concentrations of ethanol and acetonitrile. Similarly, and it can work in a wide pH and temperature range. The results of this study highlighted the potential of Bacillus licheniformis strain ML-1 as a biodegrader that can be used for the bioremediation of malathion-contaminated soil.

scholarly journals Enhancing the compost maturation of swine manure and rice straw by applying bioaugmentation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wei-Kuang Wang ◽  
Chih-Ming Liang
Keyword(s):  
Enzyme Activity ◽  
Carbon Source ◽  
Phanerochaete Chrysosporium ◽  
Bacillus Licheniformis ◽  
Enzyme Activities ◽  
Swine Manure ◽  
Bacterial Isolates ◽  
Manure Compost ◽  
Maturation Rate ◽  
Source Concentration

AbstractMicroorganisms capable of decomposing cellulose, xylan, starch and protein were individually isolated from swine manure compost and soil in this study. The correlations with pH, carbon source concentration, C/N ratio and enzyme activity among these isolated microorganisms were also investigated. Furthermore, the effect of additional inoculation in the compost was studied by measuring variations in the C/N ratio, enzyme activity and compost maturation rate. The inoculated microorganisms used in this study included four bacterial isolates and one commercial microorganism Phanerochaete chrysosporium. The results indicated that the isolated Kitasatospora phosalacinea strain C1, which is a cellulose-degraded microorganism, presented the highest enzyme activity at 31 ℃ and pH 5.5, while the C/N ratio was 0.8%. The isolated xylan-degraded microorganism Paenibacillus glycanilyticus X1 had the highest enzyme activity at 45 ℃ and pH 7.5, while the C/N ratio was 0.5%. The starch-degraded microorganism was identified as Bacillus licheniformis S3, and its highest enzyme activities were estimated to be 31 ℃ and pH 7.5 while the C/N ratio was 0.8%. The highest enzyme activity of the protein-degraded microorganism Brevinacillus agri E4 was obtained at 45 ℃ and pH 8.5, while the C/N ratio was 1.0%. The rate of temperature increase in the compost inoculated with P. chrysosporium was only higher than that of the compost without inoculation, and its compost maturation level was also lower than that of other composts with additional inoculation. The optimal initial C/N ratio of the compost was 27.5 and the final C/N ratio was 18.9. The composting results also indicated that the secondary inoculation would benefit compost maturation, and the lowest final C/N ratio of 17.0 was obtained.

scholarly journals Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source

Polymers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1959
Author(s):  
Chien Thang Doan ◽  
Thi Ngoc Tran ◽  
Thi Thanh Nguyen ◽  
Thi Phuong Hanh Tran ◽  
Van Bon Nguyen ◽  
...  
Keyword(s):  
Carbon Source ◽  
Bacillus Licheniformis ◽  
Sucrose Concentration ◽  
Value Added ◽  
Hydrolytic Activity ◽  
Culture Conditions ◽  
Glycoside Hydrolase Family ◽  
Glycoside Linkage ◽  
Optimal Medium ◽  
Value Added Products

Recently, there has been increasing use of agro-byproducts in microbial fermentation to produce a variety of value-added products. In this study, among various kinds of agro-byproducts, pomelo albedo powder (PAP) was found to be the most effective carbon source for the production of sucrose hydrolyzing enzyme by Bacillus licheniformis TKU004. The optimal medium for sucrolytic enzyme production contained 2% PAP, 0.75% NH4NO3, 0.05% MgSO4, and 0.05% NaH2PO4 and the optimal culture conditions were pH 6.7, 35 °C, 150 rpm, and 24 h. Accordingly, the highest sucrolytic activity was 1.87 U/mL, 4.79-fold higher than that from standard conditions using sucrose as the carbon source. The purified sucrolytic enzyme (sleTKU004) is a 53 kDa monomeric protein and belongs to the glycoside hydrolase family 68. The optimum temperature and pH of sleTKU004 were 50 °C, and pH = 6, respectively. SleTKU004 could hydrolyze sucrose, raffinose, and stachyose by attacking the glycoside linkage between glucose and fructose molecules of the sucrose unit. The Km and Vmax of sleTKU004 were 1.16 M and 5.99 µmol/min, respectively. Finally, sleTKU004 showed strong sucrose tolerance and presented the highest hydrolytic activity at the sucrose concentration of 1.2 M–1.5 M.

Efficient synthesis of 2-phenylethanol from L-phenylalanine by engineered Bacillus licheniformis using molasses as carbon source

2020 ◽  
Vol 104 (17) ◽  
pp. 7507-7520
Author(s):  
Yangyang Zhan ◽  
Menglin Zhou ◽  
Huan Wang ◽  
Lixia Chen ◽  
Zhi Li ◽  
...  
Keyword(s):  
Carbon Source ◽  
Bacillus Licheniformis ◽  
Efficient Synthesis

scholarly journals Microbial Purification of Postfermentation Medium after 1,3-PD Production from Raw Glycerol

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Daria Szymanowska-Powałowska ◽  
Joanna Piątkowska ◽  
Katarzyna Leja
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Organic Acids ◽  
Butyric Acid ◽  
Bacillus Licheniformis ◽  
Fermentation Medium ◽  
Alcaligenes Faecalis ◽  
Bacterial Strains ◽  
Chemical Product ◽  
Raw Glycerol

1,3-Propanediol (1,3-PD) is an important chemical product which can be used to produce polyesters, polyether, and polyurethanes. In the process of conversion of glycerol to 1,3-PD byClostridiumlarge number of byproducts (butyric, acetic and lactic acid) are generated in the fermentation medium. The aim of this work was to isolate bacteria strains capable of the utilization of these byproducts. Screening of 30 bacterial strains was performed using organic acids as carbon source. Selected isolates were taxonomically characterized and identified asAlcaligenes faecalisandBacillus licheniformis. The most active strains,Alcaligenes faecalisJP1 andBacillus licheniformisJP19, were able to utilize organic acids almost totally. Finally, it was find out that by the use of coculture (C. butyricumDSP1 andA. faecalisJP1) increased volumetric productivity of 1,3-PD production (1.07 g/L/h) and the yield equal to 0.53 g/g were obtained in bioreactor fermentation. Moreover, the only by-product present was butyric acid in a concentration below 1 g/L.

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