Microbial consortia adaptation to substrate changes in anaerobic digestion

Evaluation of bioelectrochemical systems for wastewater treatment and sludge digestion

10.32469/10355/45850 ◽

2014 ◽

Author(s):

◽

Shashikanth Gajaraj

Keyword(s):

Wastewater Treatment ◽

Anaerobic Digestion ◽

Membrane Fouling ◽

Microbial Fuel Cells ◽

Microbial Consortia ◽

Electrolysis Cell ◽

Bioelectrochemical Systems ◽

Wastewater Remediation ◽

Sludge Digestion ◽

Removal Efficiencies

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Much attention has been drawn by bioelectrochemical systems (BES) in the past years for wastewater treatment, due to its potential for enhanced wastewater treatment and resource recovery with added advantages in terms of energy generation, environmental footprint, operating stability and economics. This dissertation focuses on the potential to improve treatment efficiency of different wastewater components when assisted by BES. Modified Ludzack-Ettinger (MLE) process and membrane bioreactor (MBR) process assisted by microbial fuel cells (MFC) showed improved the nitrate-nitrogen removal efficiencies by upto 31% and 20% respectively, and reduced sludge produced by 11% and 6% respectively over the control reactors. While the unique design of the cathode significantly reduced physical membrane fouling, all other bioreactor performance was unaffected. Microbial electrolysis cell (MEC) assisted Cr[VI] reduction was faster in 60 days as compared to 69 days with MFC assisted systems and 85 days with the control. The total Cr removal efficiencies in the control system and the MFC or MEC-assisted systems were 20%, 55%, and 65%, respectively, demonstrating the ability of BES in assisting wastewater remediation process. Finally, MECs incorporated into anaerobic digestion resulted in increased production of methane of 9.4 % or 8.5% with both glucose and activated sludge respectively as the substrate. The integration of MEC had no impact on acetoclastic methanogens involved in anaerobic digestion, but significantly increased the populations of hydrogenotrophic methanogens, especially Methanobacteriales. In conclusion, the integration of BES with conventional wastewater treatment and sludge digestion process enhanced the removal of organic matter, nitrate and toxic metals while supporting healthy microbial consortia.

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A Review on Anaerobic Co-Digestion with a Focus on the Microbial Populations and the Effect of Multi-Stage Digester Configuration

Energies ◽

10.3390/en12061106 ◽

2019 ◽

Vol 12 (6) ◽

pp. 1106 ◽

Cited By ~ 50

Author(s):

Anahita Rabii ◽

Saad Aldin ◽

Yaser Dahman ◽

Elsayed Elbeshbishy

Keyword(s):

Anaerobic Digestion ◽

Microbial Population ◽

System Stability ◽

Microbial Consortia ◽

Microbial Populations ◽

Stage Versus ◽

Two Stage ◽

Waste Sludge ◽

Multi Stage ◽

Mode A

Recent studies have shown that anaerobic co-digestion (AnCoD) is superior to conventional anaerobic digestion (AD). The benefits of enhanced bioenergy production and solids reduction using co-substrates have attracted researchers to study the co-digestion technology and to better understand the effect of multi substrates on digester performance. This review will discuss the results of such studies with the main focus on: (1) generally the advantages of co-digestion over mono-digestion in terms of system stability, bioenergy, and solids reduction; (2) microbial consortia diversity and their synergistic impact on biogas improvement; (3) the effect of digester mode, i.e., multi-stage versus single stage digestion on AnCoD. It is essential to note that the studies reported improvement in the synergy and diverse microbial consortia when using co-digestion technologies, in addition to higher biomethane yield when using two-stage mode. A good example would be the co-digestion of biodiesel waste and glycerin with municipal waste sludge in a two-stage reactor resulting in 100% increase of biogas and 120% increase in the methane content of the produced biogas with microbial population dominated by Methanosaeta and Methanomicrobium.

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Effects of pretreatments on anaerobic digestion performances in batch reactor for pig mortality and microbial consortia management

Biomass and Bioenergy ◽

10.1016/j.biombioe.2020.105652 ◽

2020 ◽

Vol 140 ◽

pp. 105652

Author(s):

Binbin Tang ◽

Guoqing Li ◽

Mimi He ◽

Jianfeng Liu ◽

Junlin Ji ◽

...

Keyword(s):

Anaerobic Digestion ◽

Batch Reactor ◽

Microbial Consortia

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Enhanced anaerobic digestion performance by two artificially constructed microbial consortia capable of woody biomass degradation and chlorophenols detoxification

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2020.122076 ◽

2020 ◽

Vol 389 ◽

pp. 122076 ◽

Cited By ~ 7

Author(s):

Sameh S. Ali ◽

Michael Kornaros ◽

Alessandro Manni ◽

Jianzhong Sun ◽

Abd El-Raheem R. El-Shanshoury ◽

...

Keyword(s):

Anaerobic Digestion ◽

Woody Biomass ◽

Microbial Consortia ◽

Biomass Degradation

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Effects of shock loading versus stepwise acclimation on microbial consortia during the anaerobic digestion of glycerol

Biomass and Bioenergy ◽

10.1016/j.biombioe.2016.02.001 ◽

2016 ◽

Vol 86 ◽

pp. 129-135 ◽

Cited By ~ 14

Author(s):

Juan Vásquez ◽

Kiyohiko Nakasaki

Keyword(s):

Anaerobic Digestion ◽

Shock Loading ◽

Microbial Consortia

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Mathematical Analysis and Update of ADM1 Model for Biomethane Production by Anaerobic Digestion

Fermentation ◽

10.3390/fermentation7040237 ◽

2021 ◽

Vol 7 (4) ◽

pp. 237

Author(s):

Stefano Bertacchi ◽

Mika Ruusunen ◽

Aki Sorsa ◽

Anu Sirviö ◽

Paola Branduardi

Keyword(s):

Mathematical Modeling ◽

Anaerobic Digestion ◽

Mathematical Analysis ◽

Microbial Growth ◽

Point Of View ◽

Microbial Consortia ◽

Complex Nature ◽

Substrate Consumption ◽

Biomethane Production ◽

Informatics Tools

Biomethane is a renewable product that can directly substitute its fossil counterpart, although its synthesis from residual biomasses has some hurdles. Because of the complex nature of both biomasses and the microbial consortia involved, innovative approaches such as mathematical modeling can be deployed to support possible improvements. The goal of this study is two-fold, as we aimed to modify a part of the Anaerobic Digestion Model No. 1 (ADM1), describing biomethane production from activated sludge, matching with its actual microbial nature, and to use the model for identifying relevant parameters to improve biomethane production. Firstly, thermodynamic analysis was performed, highlighting the direct route from glucose to biomethane as the most favorable. Then, by using MATLAB® and Simulink Toolbox, we discovered that the model fails to predict the microbiological behavior of the system. The structure of the ADM1 model was then modified by adding substrate consumption yields in equations describing microbial growth, to better reflect the consortium behavior. The updated model was tested by modifying several parameters: the coefficient of decomposition was identified to increase biomethane production. Approaching mathematical models from a microbiological point of view can lead to further improvement of the models themselves. Furthermore, this work represents additional evidence of the importance of informatics tools, such as bioprocess simulations to foster biomethane role in bioeconomy.

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Comparison of functional microbial profile between mesophilic and thermophilic anaerobic digestion of vegetable waste

10.21203/rs.3.rs-54152/v1 ◽

2020 ◽

Author(s):

Tianjie Ao ◽

Zhijie Xie ◽

Pan Zhou ◽

Xiaofeng Liu ◽

Liping Wan ◽

...

Keyword(s):

Microbial Community ◽

Anaerobic Digestion ◽

Volatile Fatty Acids ◽

Stable System ◽

Microbial Consortia ◽

Vegetable Waste ◽

Microbial Profile ◽

Hydrogenotrophic Methanogenesis ◽

Highly Correlated ◽

Stable Stage

Abstract Background With increasing accumulation of vegetable waste in China, the valorization of vegetable waste becomes an urgent concern. Based on the characteristics of high moisture content and biodegradable organic matter of vegetable waste, anaerobic digestion as an effective technique was selected to reuse this kind of agriculture waste. The anaerobic digestion performance is highly correlated with the functional microbial community. In this study, mesophilic and thermophilic digestions of vegetable waste were conducted, and dynamics of the microbial community were investigated. Results The mesophilic and thermophilic collapsed stages occurred at organic loading rates of 1.5 and 2.0 g volatile solid (VS)/(L·d), respectively, due to severe accumulation of volatile fatty acids. The mesophilic digestion exhibited a higher microbial diversity and richness than the thermophilic digestion. Syntrophic acetate-oxidizing coupled with hydrogenotrophic methanogenesis was the dominant pathway in the thermophilic stable system, and acetoclastic methanogenesis in the mesophilic stable system. The dominant acidogens, syntrophus, and methanogens were Candidatus_Cloacamonas, norank_f__Synergistaceae, Methanosaeta, and Methanosarcina in the mesophilic stable stage, and Anaerobaculum, Syntrophaceticus, Methanosarcina, and Methanothermobacter in thermophilic stable stage. Spirochaetae and Thermotogae phyla were the characteristic microorganisms in the mesophilic and thermophilic collapsed stages, respectively. Conclusions This study unveiled the distribution of the functional microbial consortia at the stable and collapsed periods of the anaerobic digestion of vegetable waste under mesophilic and thermophilic conditions to providing guidelines for the further research of anaerobic digestion of vegetable waste.

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