Effects of starvation for 4-20 days on the amino acid content of Biomphalaria glabrata as determined by cellulose HPTLC-densitometry with confirmation of identity using silica gel and RP-18 W plates with two mobile phases causing inversion of the separation mechanism

2018 ◽  
Vol 41 (6) ◽  
pp. 373-376
Author(s):  
Ellen Armour ◽  
Bernard Fried ◽  
Joseph Sherma
Keyword(s):  
Amino Acid ◽  
Silica Gel ◽  
Acid Content ◽  
Amino Acid Content ◽  
Biomphalaria Glabrata ◽  
Separation Mechanism ◽  
Mobile Phases

scholarly journals Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Rasdin Ridwan ◽  
Hairil Rashmizal Abdul Razak ◽  
Mohd Ilham Adenan ◽  
Wan Mazlina Md Saad
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Citrullus Lanatus ◽  
Amino Acid Content ◽  
Hplc Method ◽  
Efficient Separation ◽  
Rp Hplc ◽  
Mobile Phases ◽  
Freeze Dried ◽  
Arginine Concentration

Watermelons(Citrullus lanatus)are known to have sufficient amino acid content. In this study, watermelons grown and consumed in Malaysia were investigated for their amino acid content, L-citrulline and L-arginine, by the isocratic RP-HPLC method. Flesh and rind watermelons were juiced, and freeze-dried samples were used for separation and quantification of L-citrulline and L-arginine. Three different mobile phases, 0.7% H3P04, 0.1% H3P04, and 0.7% H3P04 : ACN (90 : 10), were tested on two different columns using Zorbax Eclipse XDB-C18and Gemini C18with a flow rate of 0.5 mL/min and a detection wavelength at 195 nm. Efficient separation with reproducible resolution of L-citrulline and L-arginine was achieved using 0.1% H3P04on the Gemini C18column. The method was validated and good linearity of L-citrulline and L-arginine was obtained withR2= 0.9956,y=0.1664x+2.4142andR2=0.9912,y=0.4100x+3.4850, respectively. L-citrulline content showed the highest concentration in red watermelon of flesh and rind juice extract (43.81 mg/g and 45.02 mg/g), whereas L-arginine concentration was lower than L-citrulline, ranging from 3.39 to 11.14 mg/g. The isocratic RP-HPLC method with 0.1% H3P04on the Gemini C18column proved to be efficient for separation and quantification of L-citrulline and L-arginine in watermelons.

scholarly journals The effects of insulin and adrenaline on the amino-acid content of blood

1933 ◽  
Vol 27 (5) ◽  
pp. 1648-1654 ◽  
Author(s):  
James Murray Luck ◽  
Stanley Wallace Morse
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Amino Acid Content ◽  
Soybean Seeds ◽  
Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

Sign in / Sign up

Export Citation Format

Share Document