Microbial communities in uranium mine tailings and mine water sediment from Jaduguda U mine, India: A culture independent analysis

The composition of microbial communities in five acid mine water samples were studied, using culture-independent 16S rDNA based cloning and restriction fragment length polymorphism (RFLP) analysis. Phylogenetic analysis revealed that the bacteria in these five samples fell into 4 major groups: Proteobacteria, Nitrospira, Firmicutes and Bacteroidetes. Proteobacteria organisms such as A. ferrooxidans appeared in samples SX3, K1 and K2, but was scarce in samples SX1 and SX2; Nitrospira organisms Leptospirillum ferrooxidans, Leptospirillum ferriphilum and Leptospirillum group III, were prevalent in samples SX1, K1 and K2, but with fewer in samples SX2 and SX3. Archaea were only detected in samples K1 and K2 from the Tong shankou copper mine. Thermoplasma and Ferroplasma lineages were detected abundantly in these two samples. Meanwhile, the results of Principal Component Analysis (PCA) based on the percentages of OTUs and data of biogeochemical parameters, revealed that biogeochemical properties affected the diversity of microbial communities in mine water. The pH, temperature and different concentrations of elements such as S, Ni, Co and Cu seemed to be key factors resulting in the diverse distribution of microbes.

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Microbial communities in low permeability, high pH uranium mine tailings: characterization and potential effects

Journal of Applied Microbiology ◽

10.1111/jam.12180 ◽

2013 ◽

Vol 114 (6) ◽

pp. 1671-1686 ◽

Cited By ~ 36

Author(s):

V.F. Bondici ◽

J.R. Lawrence ◽

N.H. Khan ◽

J.E. Hill ◽

E. Yergeau ◽

...

Keyword(s):

Microbial Communities ◽

Mine Tailings ◽

Uranium Mine ◽

Low Permeability ◽

High Ph

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Culture-independent analysis of the bacterial community in Chinese fermented vegetables and genomic analysis of lactic acid bacteria

Archives of Microbiology ◽

10.1007/s00203-021-02375-7 ◽

2021 ◽

Author(s):

Jianming Zhang ◽

Hye Seon Song ◽

Chengcheng Zhang ◽

Yeon Bee Kim ◽

Seong Woon Roh ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Bacterial Community ◽

Genomic Analysis ◽

Independent Analysis ◽

Culture Independent ◽

Fermented Vegetables

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Culture-Independent Analysis of Indomethacin-Induced Alterations in the Rat Gastrointestinal Microbiota

Applied and Environmental Microbiology ◽

10.1128/aem.00378-06 ◽

2006 ◽

Vol 72 (10) ◽

pp. 6707-6715 ◽

Cited By ~ 38

Author(s):

Andrew B. Dalby ◽

Daniel N. Frank ◽

Allison L. St. Amand ◽

Alison M. Bendele ◽

Norman R. Pace

Keyword(s):

Small Intestine ◽

Lymph Nodes ◽

Small Subunit ◽

Rrna Genes ◽

Small Subunit Rrna ◽

Mesenteric Lymph Nodes ◽

Gastrointestinal Microbiota ◽

Mesenteric Lymph ◽

Independent Analysis ◽

Culture Independent

ABSTRACT Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed for a variety of inflammatory conditions; however, the benefits of this class of drugs are accompanied by deleterious side effects, most commonly gastric irritation and ulceration. NSAID-induced ulceration is thought to be exacerbated by intestinal microbiota, but previous studies have not identified specific microbes that contribute to these adverse effects. In this study, we conducted a culture-independent analysis of ∼1,400 bacterial small-subunit rRNA genes associated with the small intestines and mesenteric lymph nodes of rats treated with the NSAID indomethacin. This is the first molecular analysis of the microbiota of the rat small intestine. A comparison of clone libraries and species-specific quantitative PCR results from rats treated with indomethacin and untreated rats revealed that organisms closely related to Enterococcus faecalis were heavily enriched in the small intestine and mesenteric lymph nodes of the treated rats. These data suggest that treatment of NSAID-induced ulceration may be facilitated by addressing the microbiological imbalances.

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