scholarly journals Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

2019 ◽  
Vol 33 (1) ◽  
pp. 54-63 ◽  
Author(s):  
Yong Zhou ◽  
Lifang Hu ◽  
Jianbo Song ◽  
Lunwei Jiang ◽  
Shiqiang Liu
Keyword(s):  
Flowering Time ◽  
Cucumis Sativus ◽  
Leaf Morphology ◽  
Transgenic Arabidopsis ◽  
Mads Box ◽  
Cucumis Sativus L ◽  
Isolation And Characterization ◽  
Mads Box Gene

scholarly journals Identification and Characterization of a SEPALLATA-like MADS-box Gene from Cucumber (Cucumis sativus L.)

2019 ◽  
Vol 47 (4) ◽  
pp. 1168-1177 ◽  
Author(s):  
Yanwei CHENG ◽  
Peng HE ◽  
Lunwei JIANG ◽  
Shiqiang LIU ◽  
Yong ZHOU
Keyword(s):  
Cucumis Sativus ◽  
Growth And Development ◽  
Ectopic Expression ◽  
Digital Object Identifier ◽  
Amino Acid Residues ◽  
Mads Box ◽  
Cucumis Sativus L ◽  
Mads Box Gene ◽  
Identification And Characterization

SEPALLATA (SEP) genes encode the E class MADS-box transcription factors that play vital roles in various aspects of plant growth and development. In this study, we isolated a SEP-like gene from cucumber (Cucumis sativus L.), which was previously named as CsMADS01. CsMADS01 had a coding sequence (CDS) of 741 bp, and coded a deduced protein of 246 amino acid residues that was predicted to be located in the nucleus. The putative CsMADS01 protein was typically characterized by the MIKC-type MADS (including MADS, I, K, and C domains) and shared high homology with other SEP-like proteins. Phylogenetic analysis of CsMADS01 and SEP proteins from other plants revealed that CsMADS01 was a member of the SEP1/2 clade of SEP proteins and was the most closely related to ZjMADS48 from Chinese jujube. Promoter analysis showed that several cis-elements related to stress response and hormones were present in the promoter region of CsMADS01. Expression analysis indicated that CsMADS01 was highly expressed during the development of male and female flowers, and the expression increased gradually along with fruit development. Ectopic expression of CsMADS01 in Arabidopsis resulted in a phyllody-like phenotype and the transgenic plants never flowered. These results suggest that CsMADS01 plays an important role in the growth and development of cucumber.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

scholarly journals Isolation and Characterization of a MADS-box Gene cDNA, PnMADS1, That is Expressed in Both Vegetative and Floral Meristems of Pharbitis nil.

2000 ◽  
Vol 17 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Michiyuki ONO ◽  
Kimiyo SAGE-ONO ◽  
Wakako MARUYAMA ◽  
Michihiro KAWAKAMI ◽  
Masayasu INOUE ◽  
...  
Keyword(s):  
Pharbitis Nil ◽  
Mads Box ◽  
Isolation And Characterization ◽  
Mads Box Gene ◽  
Floral Meristems

scholarly journals Isolation and characterization of the C-class MADS-box gene involved in the formation of double flowers in Japanese gentian

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Takashi Nakatsuka ◽  
Misa Saito ◽  
Eri Yamada ◽  
Kohei Fujita ◽  
Noriko Yamagishi ◽  
...  
Keyword(s):  
Mads Box ◽  
Isolation And Characterization ◽  
Japanese Gentian ◽  
Mads Box Gene

scholarly journals A Cucumber AGAMOUS-LIKE 15 (AGL15) MADS-Box Gene Mediates Abnormal Leaf Morphology in Arabidopsis

Agronomy ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 265
Author(s):  
Yong Zhou ◽  
Lingli Ge ◽  
Lifang Hu ◽  
Yingui Yang ◽  
Shiqiang Liu
Keyword(s):  
Functional Characterization ◽  
Ectopic Expression ◽  
Leaf Size ◽  
Reproductive Organs ◽  
Mads Box ◽  
Cucumis Sativus L ◽  
Reading Frame ◽  
Relationship Analysis ◽  
Mads Box Gene

The AGL15 subfamily MADS-box proteins play vital roles in various developmental processes, such as floral transition, somatic embryogenesis, and leaf and fruit development. In this work, an AtAGL15 ortholog, CsMADS26, was cloned from cucumber (Cucumis sativus L.). The open reading frame (ORF) of CsMADS26 is 669 bp in length, encoding a predicted protein of 222 amino acids. The CsMADS26 protein contains a highly conserved MADS-box domain and a variable C domain, as well as less conserved I and K domains. Phylogenetic relationship analysis revealed that CsMADS26 was clustered into the AGL15 clade of AGL15 subfamily. Expression analysis based on qRT-PCR showed that CsMADS26 is mainly expressed in reproductive organs including flowers and fruits. Transgenic Arabidopsis plants with ectopic expression of CsMADS26 exhibited curled rosette and cauline leaves, and the leaf size was much smaller than that of wild-type (WT) plants. These results provide clues for the functional characterization of CsMADS26 in the future.

scholarly journals Isolation and Functional Characterization of an AGAMOUS-LIKE 18 (AGL18) MADS-box Gene from Cucumber (Cucumis sativus L.)

2018 ◽  
Vol 47 (2) ◽  
pp. 300-307
Author(s):  
Yong ZHOU ◽  
Lifang HU ◽  
Lingli GE ◽  
Peng HE ◽  
Yingui YANG ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Functional Characterization ◽  
Amino Acid Identity ◽  
Leaf Length ◽  
Pcr Analysis ◽  
Width Ratio ◽  
Mads Box ◽  
Phylogenetic Tree Analysis ◽  
Cucumis Sativus L ◽  
Mads Box Gene

MADS-box proteins play vital roles in plant growth and development. However, few studies have addressed the biological functions of MADS-box genes in cucumber. In this study, a MADS-box gene, CsMADS25, was cloned from cucumber (Cucumis sativus L.). The open reading frame (ORF) of CsMADS25 was 810 bp in length and encoded a deduced protein consisting of 269 amino acids with a calculated MW of 30.53 kDa and a theoretical pI of 5.38. Sequence alignment showed that CsMADS25 shared the highest amino acid identity with CmMADS09 from Cucumis melo. Phylogenetic tree analysis indicated that CsMADS25 was clustered with AGL18 proteins with high bootstrap values. qRT-PCR analysis showed that the expression of CsMADS25 was observably regulated by various abiotic stresses and GAtreatments. Overexpression of CsMADS25 resulted in dwarf and small-leaf phenotypes in transgenic Arabidopsis plants, and the leaf index value (leaf length/width ratio) of transgenic plants was dramatically increased compared with that of wild-type (WT) plants. These findings suggest that CsMADS25 might play important roles in various developmental processes and in response to abiotic stress of cucumber.

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