Isolation and Functional Characterization of an AGAMOUS-LIKE 18 (AGL18) MADS-box Gene from Cucumber (Cucumis sativus L.)

MADS-box proteins play vital roles in plant growth and development. However, few studies have addressed the biological functions of MADS-box genes in cucumber. In this study, a MADS-box gene, CsMADS25, was cloned from cucumber (Cucumis sativus L.). The open reading frame (ORF) of CsMADS25 was 810 bp in length and encoded a deduced protein consisting of 269 amino acids with a calculated MW of 30.53 kDa and a theoretical pI of 5.38. Sequence alignment showed that CsMADS25 shared the highest amino acid identity with CmMADS09 from Cucumis melo. Phylogenetic tree analysis indicated that CsMADS25 was clustered with AGL18 proteins with high bootstrap values. qRT-PCR analysis showed that the expression of CsMADS25 was observably regulated by various abiotic stresses and GAtreatments. Overexpression of CsMADS25 resulted in dwarf and small-leaf phenotypes in transgenic Arabidopsis plants, and the leaf index value (leaf length/width ratio) of transgenic plants was dramatically increased compared with that of wild-type (WT) plants. These findings suggest that CsMADS25 might play important roles in various developmental processes and in response to abiotic stress of cucumber.

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Isolation of a MADS-box gene (ERAF17) and correlation of its expression with the induction of formation of female flowers by ethylene in cucumber plants (Cucumis sativus L.)

Planta ◽

10.1007/s004250100571 ◽

2001 ◽

Vol 213 (6) ◽

pp. 943-952 ◽

Cited By ~ 36

Author(s):

Sugihiro Ando ◽

Yuka Sato ◽

Shin'ichiro Kamachi ◽

Shingo Sakai

Keyword(s):

Cucumis Sativus ◽

Mads Box ◽

Cucumis Sativus L ◽

Mads Box Gene ◽

Female Flowers

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Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2018.1534556 ◽

2019 ◽

Vol 33 (1) ◽

pp. 54-63 ◽

Cited By ~ 2

Author(s):

Yong Zhou ◽

Lifang Hu ◽

Jianbo Song ◽

Lunwei Jiang ◽

Shiqiang Liu

Keyword(s):

Flowering Time ◽

Cucumis Sativus ◽

Leaf Morphology ◽

Transgenic Arabidopsis ◽

Mads Box ◽

Cucumis Sativus L ◽

Isolation And Characterization ◽

Mads Box Gene

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A Cucumber AGAMOUS-LIKE 15 (AGL15) MADS-Box Gene Mediates Abnormal Leaf Morphology in Arabidopsis

Agronomy ◽

10.3390/agronomy8110265 ◽

2018 ◽

Vol 8 (11) ◽

pp. 265

Author(s):

Yong Zhou ◽

Lingli Ge ◽

Lifang Hu ◽

Yingui Yang ◽

Shiqiang Liu

Keyword(s):

Functional Characterization ◽

Ectopic Expression ◽

Leaf Size ◽

Reproductive Organs ◽

Mads Box ◽

Cucumis Sativus L ◽

Reading Frame ◽

Relationship Analysis ◽

Mads Box Gene

The AGL15 subfamily MADS-box proteins play vital roles in various developmental processes, such as floral transition, somatic embryogenesis, and leaf and fruit development. In this work, an AtAGL15 ortholog, CsMADS26, was cloned from cucumber (Cucumis sativus L.). The open reading frame (ORF) of CsMADS26 is 669 bp in length, encoding a predicted protein of 222 amino acids. The CsMADS26 protein contains a highly conserved MADS-box domain and a variable C domain, as well as less conserved I and K domains. Phylogenetic relationship analysis revealed that CsMADS26 was clustered into the AGL15 clade of AGL15 subfamily. Expression analysis based on qRT-PCR showed that CsMADS26 is mainly expressed in reproductive organs including flowers and fruits. Transgenic Arabidopsis plants with ectopic expression of CsMADS26 exhibited curled rosette and cauline leaves, and the leaf size was much smaller than that of wild-type (WT) plants. These results provide clues for the functional characterization of CsMADS26 in the future.

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Identification and Characterization of a SEPALLATA-like MADS-box Gene from Cucumber (Cucumis sativus L.)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47411610 ◽

2019 ◽

Vol 47 (4) ◽

pp. 1168-1177 ◽

Cited By ~ 1

Author(s):

Yanwei CHENG ◽

Peng HE ◽

Lunwei JIANG ◽

Shiqiang LIU ◽

Yong ZHOU

Keyword(s):

Cucumis Sativus ◽

Growth And Development ◽

Ectopic Expression ◽

Digital Object Identifier ◽

Amino Acid Residues ◽

Mads Box ◽

Cucumis Sativus L ◽

Mads Box Gene ◽

Identification And Characterization

SEPALLATA (SEP) genes encode the E class MADS-box transcription factors that play vital roles in various aspects of plant growth and development. In this study, we isolated a SEP-like gene from cucumber (Cucumis sativus L.), which was previously named as CsMADS01. CsMADS01 had a coding sequence (CDS) of 741 bp, and coded a deduced protein of 246 amino acid residues that was predicted to be located in the nucleus. The putative CsMADS01 protein was typically characterized by the MIKC-type MADS (including MADS, I, K, and C domains) and shared high homology with other SEP-like proteins. Phylogenetic analysis of CsMADS01 and SEP proteins from other plants revealed that CsMADS01 was a member of the SEP1/2 clade of SEP proteins and was the most closely related to ZjMADS48 from Chinese jujube. Promoter analysis showed that several cis-elements related to stress response and hormones were present in the promoter region of CsMADS01. Expression analysis indicated that CsMADS01 was highly expressed during the development of male and female flowers, and the expression increased gradually along with fruit development. Ectopic expression of CsMADS01 in Arabidopsis resulted in a phyllody-like phenotype and the transgenic plants never flowered. These results suggest that CsMADS01 plays an important role in the growth and development of cucumber. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Overexpression of an APETALA1-like gene from cucumber (Cucumis sativus L.) induces earlier flowering and abnormal leaf development in transgenic Arabidopsis

Canadian Journal of Plant Science ◽

10.1139/cjps-2018-0107 ◽

2019 ◽

Vol 99 (2) ◽

pp. 210-220 ◽

Cited By ~ 1

Author(s):

Yong Zhou ◽

Lifang Hu ◽

Shuifeng Ye ◽

Lunwei Jiang ◽

Shiqiang Liu

Keyword(s):

Cucumis Sativus ◽

Plant Development ◽

Flower Development ◽

Leaf Development ◽

Molecular Mechanisms ◽

Transgenic Arabidopsis ◽

Functional Characterization ◽

Ectopic Expression ◽

Mads Box ◽

Cucumis Sativus L

MADS-box proteins are important transcription factors that play essential roles in various aspects of plant development, particularly in flower development. In this study, we performed the identification and functional characterization of CsMADS09 isolated from cucumber (Cucumis sativus L.). CsMADS09 contains a 648-bp open reading frame encoding 215 amino acid residues, and shares high sequence identities with the members of the AP1/FUL family of MADS-box proteins, especially the euAPETALA1 (euAP1) subclade. Many cis-elements related to plant development, stress response, and hormones were identified in the promoter region of CsMADS09. Quantitative real-time polymerase chain reaction results showed that CsMADS09 was mainly expressed in reproductive tissues such as male flowers and unexpanded ovaries, while its expression was low in roots and only traceable in fertilized ovaries. Moreover, the results revealed that CsMADS09 expression tended to decline during male flower development and stayed nearly constant during female flower development. Ectopic expression of CsMADS09 resulted in earlier flowering and abnormal leaf development in transgenic Arabidopsis. This study is the first functional analysis of an AP1-like gene from cucumber and provides some clues for revealing the molecular mechanisms of flower development in cucumber.

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Molecular Cloning and Expression Analysis of a MADS-Box Gene (GbMADS2) from Ginkgo biloba

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4319760 ◽

2015 ◽

Vol 43 (1) ◽

pp. 19-24 ◽

Cited By ~ 3

Author(s):

Xiaohui WANG ◽

Junhuan CHENG ◽

Feng XU ◽

Xingxiang LI ◽

Weiwei ZHANG ◽

...

Keyword(s):

Expression Analysis ◽

Ginkgo Biloba ◽

Flower Development ◽

Time Course ◽

Male Flower ◽

Cloning And Expression ◽

Pcr Analysis ◽

Mads Box ◽

Typical Structure ◽

Mads Box Gene

As a kind of transcription factors gene family, MADS-box genes play an important role in plant development processes. To find genes involved in the floral transition of Ginkgo biloba, a MADS-box gene, designated as GbMADS2, was cloned from G. biloba based on EST sequences by RT-PCR. Sequence analysis results showed that the cDNA sequence of GbMADS2 contained a 663 bp length ORF encoding 221 amino acids protein, which displayed typical structure of plant MADS-box protein including MADS, I, and K domains and C terminus. The sequence of GbMADS2 protein was highly homologous to those of MADS-box proteins from other plant species with the highest homologous to AGAMOUS (CyAG) from Cycas revoluta. The phylogenetic tree analysis revealed that GbMADS2 belonged to AGAMOUS clade genes. Real-time PCR analysis indicated that expression levels of GbMADS2 gene in female and male flower were significantly higher than those in root, stem, and leaves, and that GbMADS2 expression level increased along with time of flower development. The spatial and time-course expression profile of GbMADS2 implied that GbMADS2 might be involved in development of reproductive organs. The isolation and expression analysis of GbMADS2 provided basis for further studying the molecular mechanism of flower development in G. biloba.

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