In a number of manufacturing processes, tooling installation, calibration and maintenance guarantee the precision of fixtures and play important roles towards the overall quality of products. Recently a new type of measurement equipment called “laser tracker” was developed and utilized for assembly fixture calibration to shorten calibration time and improve the accuracy of the currently used theodolite systems. Though, the calibration of assembly fixture is critical for product quality, the calibration time creates significant burden for productivity of multi-station assembly processes. In order to shorten the calibration lead time, the number of necessary calibration setups which is determined by visibility analysis needs to be minimized. This paper presents a screen space transformation based visibility analysis that allows minimizing the number of setups. The screen space transformation is applied to transform the visibility problem from 3D to 2D space, consequently the visibility problem can be solved efficiently. A case study illustrates the procedure and verifies the validity of the proposed methodology.