The article is devoted to the study of effective websites design principles, adherence to which is very important and necessary in the modern information space. The article analyzes scientific research on the above problem and its results. The author defines the concept of usability, and describes its main indicators. The article considers the most important features of the user’s perception of interfaces and formulates the rules of usability through the organization of screen space. It is emphasized that the basic rules of website design are based on knowledge of the human perception mechanisms. Users’ psychophysical processes influence visual perception of screen space. Therefore, one needs to consider the psychology of human perception of the computer, the theory of information transfer, and also human psychophysiology to create highly usable interfaces and achieve effective users’ interactions with websites. All this reduces the cognitive load and time for information search, and meets users’ needs in the most convenient way. In addition, the principles of organizing web text in a way that ensures the best perception of information by the user are defined, in particular: structure, visualization, laconism and clarity. The article reveals the significance of website usability, describes criteria and methods of its assessment. Among the indicators used in website evaluation, the following are highlighted: effectiveness, navigation convenience, memorability. The author describes the methods for evaluating the effectiveness of the website, namely: statistics analysis, collection and analysis of visitors’ opinions, observing users’ actions, engaging users in testing. It is proved that the observance of usability principles results in an increased number of visitors and time they spend on a website, higher conversion rate, etc. The principles described in the article are generalization of the world experience of website design professionals.