High-intensity interval training (HIIT) effectively enhances heart function via miR-195 dependent cardiomyopathy reduction in high-fat high-fructose diet-induced diabetic rats

This study demonstrates the effect of a single high-intensity interval training (HIIT) session on the redox status of rat ovaries with excess adiposity. Forty Wistar female rats (mean (±s.e.m.) weight 94.40 ± 13.40 g) were divided into two groups and fed either a standard diet (SD) or a high-fat diet (HFD) for 62 days. At the end of this period, the rats were subjected to a single HIIT session and were killed 24 h after exercise. Both groups subjected to exercise (SDex and HFDex) generated a significantly higher antioxidant environment by presenting a higher thiol content, which represents a lower oxidation rate of GSH than their respective controls (SD and HFD). The percentage of morphologically normal primary follicles decreased, whereas that of antral follicles increased, in the SDex group. In addition, the HFD group had a higher percentage of degenerated antral follicles than the SD and SDex groups. Cells immunoreactive for α-smooth muscle actin were seen in the cortical stroma and thecal layer enclosing late secondary and tertiary follicles in all groups. Moreover, heme oxygenase and cytochrome P450 family 19 subfamily A member 1 (Cyp19A1) labelling was seen in all antral follicles. Progesterone concentrations were significantly higher in the HFDex than SDex group. In conclusion, this study indicates that a single session of HIIT may result in an improvement in ovary redox status because of metabolic muscle activity by inducing physiological adaptation after exercise in a paracrine manner.

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The effect of high-intensity interval training (HIIT) on gene expression of the factors involved in the skeletal muscle metabolism of diabetic rats

Pars of Jahrom University of Medical Sciences ◽

10.52547/jmj.17.2.32 ◽

2019 ◽

Vol 17 (2) ◽

pp. 32-39

Author(s):

mona Bostan manesh nik javan ◽

saeedeh shadmehri ◽

mozhgan ahmadi ◽

◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

High Intensity ◽

Muscle Metabolism ◽

Interval Training ◽

Diabetic Rats ◽

High Intensity Interval Training ◽

Skeletal Muscle Metabolism ◽

High Intensity Interval

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High-intensity interval training improved fasting blood glucose and lipid profiles in type 2 diabetic rats more than endurance training; possible involvement of irisin and betatrophin

Physiology International ◽

10.1556/2060.106.2019.24 ◽

2019 ◽

Vol 106 (3) ◽

pp. 213-224 ◽

Cited By ~ 5

Author(s):

J Amri ◽

M Parastesh ◽

M Sadegh ◽

SA Latifi ◽

M Alaee

Keyword(s):

Blood Glucose ◽

High Intensity ◽

Fasting Blood Glucose ◽

Interval Training ◽

Diabetic Rats ◽

Lipid Profiles ◽

High Intensity Interval Training ◽

Training Groups ◽

High Intensity Interval

Background and aims In this study, we aimed to investigate the effects of 10 weeks of high-intensity interval training (HIIT) and endurance training (END) on irisin, betatrophin, insulin, fasting blood glucose (FBG) concentrations, and lipid profiles in diabetic rats. Methods Twenty-four Wistar rats (weight: 200–250 g) were randomly assigned into four groups as follows: (1) control (Cnt), (2) diabetic (Dibt), (3) diabetic HIIT (Dibt-HIIT), and (4) diabetic END (Dibt-END). For inducing diabetes, after 12 h of food starvation, nicotinamide (120 mg/kg) and streptozotocin (STZ; 65 mg/kg) were intraperitoneally injected. The diabetic training groups received 10 weeks of HIIT or END training following the induction of diabetes. Twenty-four hours following the last training session, blood serum samples were collected for evaluating the concentration of irisin, betatrophin, and insulin hormones through enzyme-linked immunosorbent assay. Results FBG and lipid profiles were measured by biochemical kits. A significant increase in the serum concentration of irisin (p < 0.05), betatrophin (p < 0.05), and insulin (p < 0.001) and significant decrease in the FBG (P < 0.01) and lipid profiles (p < 0.01) were observed in the Dibt-HIIT group compared to the Dibt-END group. In addition, irisin revealed a significant positive association with betatrophin and insulin values in diabetic training groups (p < 0.01). Conclusions It seems that HIIT leads to a more extensive improvement in diabetic conditions compared to the END training. Therefore, HIIT appears to be an important time-efficient approach for the treatment of type 2 diabetes.

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Effects of High Intensity Interval Training on the Gene Expression of PGC1-Α, CS and P-53 in the Cardiomyocyte of Male Obese Rats in Type 2 Diabetes

Journal of Shahid Sadoughi University of Medical Sciences ◽

10.18502/ssu.v28i11.5222 ◽

2021 ◽

Author(s):

Nadia Khayampour ◽

Maghsoud Peeri ◽

Mohammad Ali Azarbayjani ◽

Maryam Delfan

Keyword(s):

High Intensity ◽

Interval Training ◽

Diabetic Rats ◽

Diabetic Patients ◽

High Intensity Interval Training ◽

Healthy Control ◽

High Intensity Interval ◽

Obese Male ◽

P 53

Introduction: Exercise training with different intensity regulates metabolism at the cellular level by regulating the expression of genes involved in mitochondrial biogenesis in diabetic patients. The aim of this study was to evaluate the effect of 4 weeks of high intensity interval training on the expression of PGC-1α, CS and p-53 genes in the cardiomyocytes of obese male rats with type 2 diabetes. Methods: The present study was an experimental one. Eighteen obese male diabetic rats were divided into three groups of six: high intensity interval training (HIIT), diabetic control (DC), healthy control (NC). Diabetes was induced in all groups except the healthy control group by streptozotocin (STZ) injection. After anesthesia, blood serum was obtained directly from their left ventricle and immediately extracted from their left ventricle. Plasma glucose was measured by glucose oxidase assay. To determine the expression of PGC-1α, CS and P-53 genes, PCR-Real time method and group comparison were used by one-way ANOVA test with application 8 version graph pad prism at alpha level of 0.05. Results: The increase in PGC-1α gene expression in HIIT group compared to DC (P = 0.0001) and NC (P = 0.001) groups was significant. Increased expression of CS gene in HIIT group was significant compared to DC (P = 0.0001) and NC (P = 0.009) groups. Decreased expression of P-53 gene in HIIT group compared to DC (P = 0.0001) and NC (P = 0.001) groups were significantly different. Weight and glucose were significantly reduced in the HIIT group. Conclusion: The results showed that by increasing the PGC-1α, CS genes and decreasing the expression of P-53 gene in cardiomyocytes of obese diabetic rats, it improves the energy metabolism in diabetic patients due to mitochondrial deficiency and possibly it can improve diabetic cardiomyopathy.

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The effect of Progressive Resistance Training and High Intensity Interval Training on TRAF6 gene expression of the heart muscle and serum TNF-α levels in male diabetic rats

Medical Journal of Tabriz University of Medical Sciences ◽

10.34172/mj.2021.47 ◽

2021 ◽

Vol 43 (2) ◽

pp. 209-219

Author(s):

Seyedeh Fatemeh Tonkaboni ◽

Neda Khaledi ◽

Hossein Askari

Keyword(s):

Gene Expression ◽

Resistance Training ◽

High Intensity ◽

Interval Training ◽

Diabetic Rats ◽

Progressive Resistance Training ◽

High Intensity Interval Training ◽

Tnf Α ◽

High Intensity Interval ◽

Progressive Resistance

Background: The most common causes of mortality in diabetic patients are cardiovascular disorders, one of the reasons being inflammatory factors. Given that physical activity can reduce inflammation, the present study was to investigate the effects of two types of Progressive Resistance Training (RT) and High Intensity Interval (HIIT) on the expression of TRAF6 gene and serum levels of TNF-α in male diabetic rats. Methods: In this experimental study, 72 male rats were divided into 6 groups of 12 diabetic rats (n=12), control (n=12), diabetic High Intensity Interval Training (n=12), High Intensity Interval Training(n=12), diabetic Progressive Resistance Training(n=12) and Progressive Resistance Training(n=12). Progressive Resistance Training was performed in a 6-weekly 3-session, climbing the vertical ladder, with 50%, 75%, 90% and 100% the body weight of the animals. After successful completion, 30 gr were added to the weights, to the extent that the rats cannot carry the ladder. High Intensity Interval Training were also performed at 6-weekly 3-session, with an intensity of 50 to 110% of the VO2max. 24 hours after the completion of the training, the functional test was taken and the animals were autopsy 48 hours after the test. Finally, the expression of TRAF6 gene was evaluated using Real Time PCR and serum TNF-α level by ELISA method. Results: TRAF6 levels increased significantly after the two Training in the diabetic group, which was higher in the HIIT group and serum TNF-α levels decreased significantly after both types of training, which was more prominent in the HIIT group. Conclusion: HIIT and RT can play an important role in reducing the inflammatory factor of TNF-α in diabetic patient that HIIT is more effective in this regard. Increasing the gene expression of the mediating agent TRAF6 can be induced in inflammatory pathways, which may require a reduction in exercise intensity or in anti-inflammatory routes indicating a positive effect of training on diabetes.

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