The effect of high-intensity interval training (HIIT) on gene expression of the factors involved in the skeletal muscle metabolism of diabetic rats

Background: The most common causes of mortality in diabetic patients are cardiovascular disorders, one of the reasons being inflammatory factors. Given that physical activity can reduce inflammation, the present study was to investigate the effects of two types of Progressive Resistance Training (RT) and High Intensity Interval (HIIT) on the expression of TRAF6 gene and serum levels of TNF-α in male diabetic rats. Methods: In this experimental study, 72 male rats were divided into 6 groups of 12 diabetic rats (n=12), control (n=12), diabetic High Intensity Interval Training (n=12), High Intensity Interval Training(n=12), diabetic Progressive Resistance Training(n=12) and Progressive Resistance Training(n=12). Progressive Resistance Training was performed in a 6-weekly 3-session, climbing the vertical ladder, with 50%, 75%, 90% and 100% the body weight of the animals. After successful completion, 30 gr were added to the weights, to the extent that the rats cannot carry the ladder. High Intensity Interval Training were also performed at 6-weekly 3-session, with an intensity of 50 to 110% of the VO2max. 24 hours after the completion of the training, the functional test was taken and the animals were autopsy 48 hours after the test. Finally, the expression of TRAF6 gene was evaluated using Real Time PCR and serum TNF-α level by ELISA method. Results: TRAF6 levels increased significantly after the two Training in the diabetic group, which was higher in the HIIT group and serum TNF-α levels decreased significantly after both types of training, which was more prominent in the HIIT group. Conclusion: HIIT and RT can play an important role in reducing the inflammatory factor of TNF-α in diabetic patient that HIIT is more effective in this regard. Increasing the gene expression of the mediating agent TRAF6 can be induced in inflammatory pathways, which may require a reduction in exercise intensity or in anti-inflammatory routes indicating a positive effect of training on diabetes.

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The Effect of Detraining High Intensity Interval Training on the Expression of AKT1 and mTORc1 Genes in the Left Ventricle of Diabetic Rats

Iranian journal of diabetes and obesity ◽

10.18502/ijdo.v12i1.3637 ◽

2020 ◽

Author(s):

Farzaneh Soltanipour jounaghani ◽

Maghsoud Peeri ◽

Mohammad-Ali Azarbayjani

Keyword(s):

Gene Expression ◽

Left Ventricle ◽

High Intensity ◽

Interval Training ◽

Diabetic Rats ◽

Single Step ◽

Male Rats ◽

P Value ◽

High Intensity Interval Training ◽

High Intensity Interval

Objective: The aim of this study was to investigate the effect of 6 weeks of detraining after 12 weeks of high intensity interval training (HIIT) on the expression of AKT1 and mTORc1 genes in the left ventricle of wistar diabetic rats. Materials and Methods: Twenty-eight wistar male rats were selected as the study sample and were divided in four groups of healthy control, diabetic control, diabetic HIIT and diabetic HIIT + detraining. The HIIT period was 12 weeks and the detraining period was 6 weeks. Each session consisted of 30 minutes, which included running on a treadmill with one-minute repetitions and a two-minute active recovery between them. To measure AKT1 mRNA and mTORc1 mRNA by RT-Real time PCR, a single-step single step SYBR TAKARA kits from Takara Company was used according to the company's instruction. Results: HIIT caused a significant increase in AKT1 gene expression (P-value= 0.001). AKT1 decreased with detraining that was not significant (P-value= 0.34) but it was still significantly higher than before training (P-value= 0.017). HIIT caused a significant increase in mTORc1 gene expression (P-value= 0.001) and although it decreased with detraining (P-value= 0.15) and it was no significantly higher than before training (P-value= 0.19). Conclusion: HIIT led to increased expression of AKT1 and mTORc1 genes in type 2 diabetic rats, while also producing favorable changes in the cardiac structure of these rats. Also, 6 weeks of detraining did somewhat reduce these favorable changes.

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The Effects of High-Intensity Interval Training on Skeletal Muscle Morphological Changes and Denervation Gene expression of Aged Rats

Montenegrin Journal of Sports Science and Medicine ◽

10.26773/mjssm.190906 ◽

2019 ◽

Vol 8 (2) ◽

pp. 39-45

Author(s):

Seyed Morteza Tayebi ◽

◽

Marefat Siahkouhian ◽

Mohammad Keshavarz ◽

Mojgan Yousefi ◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

High Intensity ◽

Morphological Changes ◽

Interval Training ◽

Aged Rats ◽

High Intensity Interval Training ◽

High Intensity Interval

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Effect of 8 Weeks High Intensity Interval Training on the Gene Expression of BAX and BCL-2 in the Left Ventricle of Diabetic Male Wistar Rats

Journal of Shahid Sadoughi University of Medical Sciences ◽

10.18502/ssu.v28i7.4267 ◽

2020 ◽

Author(s):

Alireza Safarnezhad ◽

Maghsoud Peeri ◽

Mohammad Ali Azarbayjani ◽

Maryam Delfan

Keyword(s):

Gene Expression ◽

Insulin Resistance ◽

Left Ventricle ◽

High Intensity ◽

Interval Training ◽

Diabetic Rats ◽

Diabetic Patients ◽

High Intensity Interval Training ◽

High Intensity Interval ◽

Bax Gene

Introduction: Regular exercise training with alternating volume fluctuate blood glucous levels and by regulating signaling in gene expression reduces in myocardial cell apoptosis in diabetic patients. The purpose of this study was the effect of 8 weeks of high intensity interval training on the gene expression of BAX and BCL-2 in the left ventricle of diabetic rats. Methods: The present study was a quasi-experimental one. 14 male diabetic rats were divided into 2 groups of 7; high intensity interval training (HIIT) and control (C) groups. Diabetes was induced in a pellet with a high-fat diet (30% fat and 25% fructose) for 16 weeks. Twenty-four hours after the last training and recovery session, the rats were sacrificed and their left ventricle was extracted. Glucose oxidase was used to measure glucose in plasma and insulin resistance using HOMA-IR method.. PCR-Real time was used to determine the expression of BAX and BCL-2 genes and the comparison of the groups by Independent T test was performed by Graph pad prism at alpha level of 0/05. Results: Results showed that, BAX gene expression was significantly decreased in the HIIT group compared to the C group (P=0/0001). BCL-2 gene was significantly increased in the HIIT group compare to the control group (P=0/0001). Insulin resistance index and plasma glucose showed a significant decrease in the training group (P=0/01) (P=0/021). Weight did not change significantly in any of the groups. Conclusion: Based on the findings, 8 weeks high intensity interval training can be reduced apoptosis in the left ventricle of Diabetic mice by decreasing the BAX gene expression and increacing the BCL-2 in myocardial and might improve diabetes cardiomyopathy.

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