scholarly journals Ethanol extract ofCotinus coggygrialeaves accelerates wound healing process in diabetic rats

2016 ◽  
Vol 54 (11) ◽  
pp. 2732-2736 ◽  
Author(s):  
Halil Aksoy ◽  
Ali Sen ◽  
Mesut Sancar ◽  
Turgut Sekerler ◽  
Dilek Akakin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Wound Healing Process

Use of bromelain in cutaneous wound healing in streptozocin-induced diabetic rats: an experimental model

2020 ◽  
Vol 29 (9) ◽  
pp. 488-495
Author(s):  
Amene Nikgoftar Fathi ◽  
Mohammad Hassan Sakhaie ◽  
Sepehr Babaei ◽  
Soroush Babaei ◽  
Fateme Slimabad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Wound Area ◽  
Small Vessels ◽  
Histological Sections ◽  
Therapeutic Benefits ◽  
Wide Range

Objective: To assess the effect of bromelain on different aspects of the wound healing process in type 1 diabetic rats. Method: In this study, 112 streptozocin-diabetic (type 1) male Wistar rats were euthanised; 28 each on days three, five, seven and 15, after a wound incision had been made. To estimate changes in a number of different cellular and tissue elements, histological sections were provided from all wound areas and stained with haematoxylin and eosin. Some 1.056mm2 of total wound area from all specimens were evaluated, by assessment of 4200 microscope photos provided from all histological sections, by stereological methods. A biomechanical test of each wound area was performed with an extensometer to evaluate the work-up to maximum force and maximum stress of the healed wound on day 15. Results: In the experimental groups, bromleain caused significant wound contraction and reduced granulation tissue formation by day 7 (p=0.003); increased neovasculars (new small vessels that appear in the wound area during wound healing) on days three, five and seven (p=0.001); significantly increased fibroblasts on day five but decreased by day seven (p=0.002); and significantly decreased macrophage numbers and epithelium thickness on all days of study (p=0.005). Wound strength significantly increased in experimental groups by day 15. Conclusion: Bromelain has a wide range of therapeutic benefits, but in most studies the mode of its action is not properly understood. It has been proved that bromelain has no major side effects, even after prolonged use. According to the results of this study, bromelain can be used as an effective health supplement to promote and accelerate wound healing indices, reduce inflammation and improve biomechanical parameters in diabetic wounds.

scholarly journals An EGF- and Curcumin-Co-Encapsulated Nanostructured Lipid Carrier Accelerates Chronic-Wound Healing in Diabetic Rats

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4610
Author(s):  
Hye-Jin Lee ◽  
Moses Jeong ◽  
Young-Guk Na ◽  
Sung-Jin Kim ◽  
Hong-Ki Lee ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Healing Process ◽  
Double Emulsion ◽  
Average Diameter ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Nanostructured Lipid Carrier

Nanostructured lipid carriers (NLC) are capable of encapsulating hydrophilic and lipophilic drugs. The present study developed an NLC containing epidermal growth factor (EGF) and curcumin (EGF–Cur-NLC). EGF–Cur-NLC was prepared by a modified water-in-oil-in-water (w/o/w) double-emulsion method. The EGF–Cur-NLC particles showed an average diameter of 331.8 nm and a high encapsulation efficiency (81.1% and 99.4% for EGF and curcumin, respectively). In vitro cell studies were performed using two cell types, NIH 3T3 fibroblasts and HaCaT keratinocytes. The results showed no loss of bioactivity of EGF in the NLC formulation. In addition, EGF–Cur-NLC improved in vitro cell migration, which mimics the wound healing process. Finally, EGF–Cur-NLC was evaluated in a chronic wound model in diabetic rats. We found that EGF–Cur-NLC accelerated wound closure and increased the activity of antioxidant enzymes. Overall, these results reveal the potential of the NLC formulation containing EGF and curcumin to promote healing of chronic wounds.

scholarly journals Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments

2008 ◽  
Vol 53 (No. 12) ◽  
pp. 652-659 ◽  
Author(s):  
P. Gal ◽  
Kilik ◽  
R ◽  
M. Mokry ◽  
B. Vidinsky ◽  
...  
Keyword(s):  
Wound Healing ◽  
Quantitative Methods ◽  
Healing Process ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Polymorphonuclear Leucocytes ◽  
Skin Wound Healing ◽  
Simple Method

The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appropriate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment.

scholarly journals Sida cordifolia Linn. accelerates wound healing process in type 2 diabetic rats

2016 ◽  
Vol 6 (4) ◽  
pp. 82-89 ◽  
Author(s):  
Rajesh Singh Pawar ◽  
Shweta Kumar ◽  
Fedelic Ashish Toppo ◽  
Lakshmi PK ◽  
Pratibha Suryavanshi
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Diabetic Rats ◽  
Wound Healing Process ◽  
Sida Cordifolia ◽  
Type 2 Diabetic

scholarly journals Effects of Low-Intensity Laser Irradiation on Wound Healing in Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Hui Ma ◽  
Ying-xin Li ◽  
Hong-li Chen ◽  
Mei-ling Kang ◽  
Timon Cheng-Yi Liu
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Laser Irradiation ◽  
Healing Process ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Low Intensity ◽  
Intensity Laser ◽  
Later Phase

Objective.The effects of low-intensity 630 nm semiconductor laser irradiation at 3.6 J/cm2(LISL) on wound healing in diabetic rats were studied in this paper.Methods.36 diabetic rats with dorsal cutaneous excisional wounds were divided into three LISL groups and a control group randomly. The three LISL groups were irradiated with LISL at 5, 10, and 20 mW/cm2five times a week for two weeks, respectively. The process of wound healing was assessed by assessing blood glucose, calculating percentage of wound closure, histopathological evaluation, and immunohistochemical quantification.Results.Blood glucose of all groups remained at similar levels throughout the experiment. LISL could obviously promote wound contraction, fibroblasts proliferation, and collagen synthesis, alter bFGF and TGF-β1 expression, and reduce inflammatory reaction in the early and middle phases of chronic wound-healing process. However, LISL could not shorten cicatrization time, and the treatment effects were not sensitive to illuminate parameters in the later phase of the experiment.Conclusions.LISL might have auxiliary effects in the early and middle phases of wound healing in STZ-induced diabetic rats, but the reciprocity rule might not hold. The wound-healing process of early-phase diabetes rats shows typical characteristics of self-limited disease.

scholarly journals The effect of ant-plant (Hydnophytum formicarum) ethanol extract on collagen fibers for wound healing after tooth extraction in the guinea pig (Cavia cobaya)

2019 ◽  
Vol 31 (3) ◽  
pp. 189
Author(s):  
Siti Velanita ◽  
Efa Ismardianita ◽  
Andries Pascawinata
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Guinea Pig ◽  
Tooth Extraction ◽  
Healing Process ◽  
Ethanol Extract ◽  
Collagen Fibers ◽  
Control Group ◽  
Wound Healing Process ◽  
One Way Anova

Introduction: Tooth extraction causes tissue damage around the socket. Wound healing is essential for repairing damaged tissues. One of the medicinal plants often used is ant-plant (Hydnophytum formicarum), which contains flavonoids, triterpenoids, saponins and tannins. This research was aimed to determine the effect of ant-plant ethanol extract on the density of collagen fibers for wound healing after tooth extraction in the guinea pig (Cavia cobaya). Methods: The type of research was true experimental with a post-test only control group design. The study used 48 male guinea pig divided into 4 groups. Experimental groups received lower left incisor extraction, the control group was orally administered with 0.5% CMC, and the treatment group was administered with ant-plant extract dissolved with 0.5% CMC with dose of 4.65 mg, 6.2 mg, and 9.3 mg respectively; each guinea pig was given 3 cc of the extract 3 times a day until the termination day. On day 3, 7, 14, and 21 respectively, the guinea pigs were terminated and to make the histological preparations. The difference in collagen fibers density of the two observation groups was tested with one-way ANOVA, and the LSD test to analyse the significance between groups. Results: Application of ant-nest ethanol extract showed significant effects on the density of collagen fibers in the wound healing process after tooth extraction. Results of the one-way ANOVA test showed significant value on all treatment groups (p < 0.05). There were also found significant differences between the control group and the treatment group (p < 0.05). Conclusion: Ant-plant (Hydnophytum formicarum) ethanol extract increase the collagen fibers density on the wound healing after tooth extraction with the effective dose of 4.65 mg.Keywords: Ant-plant, Hydnophytum formicarum, wound healing, collagen, guinea pig, Cavia cobaya.

scholarly journals Effects of Sildenafil Citrate On Testicular Histology and Sperm Count During Wound Healing Process in Diabetic Rats

2019 ◽  
Vol 16 (1) ◽  
pp. 1-11
Author(s):  
Mohammed B. Mahre ◽  
Saidu I Ngulde ◽  
Bukar Umaru ◽  
Fakilahyel M Mshelbwala ◽  
Dauda Yahi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Sperm Count ◽  
Healing Process ◽  
Diabetic Rats ◽  
Sildenafil Citrate ◽  
Wound Healing Process ◽  
Testicular Histology

scholarly journals Efektivitas Sediaan Salep Limbah Kulit Singkong Sebagai Penyembuh Luka

2019 ◽  
Vol 5 (2) ◽  
pp. 133
Author(s):  
Widia Nila Risnadewi ◽  
Nadya Arianita Turisia ◽  
Alsifah Nurhidayati ◽  
Candra Dwipayana Hamdin
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
Ethanol Extract ◽  
Good Effect ◽  
Wound Healing Process ◽  
Tissue Integrity ◽  
Herbal Plants ◽  
Physical And Chemical

The skin is an important organ that functions to receive stimuli such as touch, pain and other influences from the outside. Some problems that occur on the skin can becaused because the skin is very vulnerable to trauma and injury. Wound is a condition of the breakdown of tissue integrity caused by violence both mechanical, physical and chemical in nature. In general, wound care in the community is done with a dressing accompanied by compresses betadine and normal saline because these materials are easily available. However, long-term use of the dressing can cause slow wound healing and various infections can appear. This causes people to look for other alternative treatments, one of them with herbal plants, namely cassava plants have the potential to be used as a wound healing and is able to inhibit bacteria because they are suspected to contain phenolic compounds. This study was a true experimental study, the group of test animals was divided into 6 groups, after that the making of wounds and smeared with bacteria were given ointments with 3 concentrations namely 5%, 10%, and 15%. Ointment extract of cassava skin waste gives effect to the wound healing process seen from the length of the wound. The ethanol extract ointment which has a good effect on wound healing is an ointment with a concentration of 10% and 15%.

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