Topical application of Cinnamomum hydroethanolic extract improves wound healing by enhancing re-epithelialization and keratin biosynthesis in streptozotocin-induced diabetic mice

Malini DM, Madihah, Kamilawati F, Ratningsih N, Alipin K, Iskandar J. 2017. Topical treatment of ointment containing ethanol extract of Archidendron pauciflorum fruit peel on the wound healing in streptozotocin-induced diabetic mice. Nusantara Bioscience 9: 306-311. Diabetic wounds lead to severe tissue damage and are difficult to cure. One alternative medicine known well by local Indonesian communities to treat diabetic wounds is the fruit peel of djengkol. This study aimed to evaluate the ointment containing ethanol extract of djengkol fruit peel to accelerate wound healing process in the skin of streptozotocin-induced diabetic mice. The method was experimental using completely randomized design with six treatments and four replications. Diabetes was induced by intraperitoneal injection of streptozotocin 180 mg/kg BW. Mice with blood glucose level â‰¥150 mg/dL were used for diabetic mice models. The incision wound created at dorsolateral region of shaven skin at Â±1 cm2 using sterile scissors. The ointment containing extract was applied topically to the diabetic mice wounds at concentration of 5%, 10% and 15%, as well as BetadineÂ® as the reference group. The ointment basis was applied to the wound of diabetic mice as a positive control and to the wound of non-diabetic mice as a negative control. The treatment was done twice a day for 14 days. The results showed that topical application of ointment containing ethanol extract of djengkol fruit peel at concentration of 10% gave significant recovery (p<0.05) on the wounded skin by the enhancement of re-epithelization and granulation tissue, as well as the increase of capillary number and collagen density which were higher than other treatments and comparable to negative control group. It was concluded that the topical application of ointment containing ethanol extract of djengkol fruit peel can support the healing of diabetic wounds.

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Topical Application of Yeast Extract Accelerates Wound Healing in Diabetic Mice

Journal of Burn Care & Rehabilitation ◽

10.1097/00004630-199801001-00007 ◽

1998 ◽

Vol 19 ◽

pp. S138

Author(s):

M J Crowe ◽

R B McNeill ◽

D J Schlem ◽

D G Greenhalgh ◽

S J Keller

Keyword(s):

Wound Healing ◽

Yeast Extract ◽

Topical Application ◽

Diabetic Mice

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Topical application of ex vivo expanded endothelial progenitor cells promotes vascularisation and wound healing in diabetic mice

International Wound Journal ◽

10.1111/j.1742-481x.2012.01010.x ◽

2012 ◽

Vol 10 (5) ◽

pp. 527-533 ◽

Cited By ~ 32

Author(s):

Jun Asai ◽

Hideya Takenaka ◽

Masaaki Ii ◽

Michio Asahi ◽

Saburo Kishimoto ◽

...

Keyword(s):

Wound Healing ◽

Progenitor Cells ◽

Endothelial Progenitor Cells ◽

Topical Application ◽

Ex Vivo ◽

Diabetic Mice ◽

Endothelial Progenitor

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Topical Application of Propolis Enhances Cutaneous Wound Healing by Promoting TGF-Beta/Smad-Mediated Collagen Production in a Streptozotocin-Induced Type I Diabetic Mouse Model

Cellular Physiology and Biochemistry ◽

10.1159/000430221 ◽

2015 ◽

Vol 37 (3) ◽

pp. 940-954 ◽

Cited By ~ 48

Author(s):

Wael N. Hozzein ◽

Gamal Badr ◽

Ahmad A. Al Ghamdi ◽

Ayat Sayed ◽

Noori S. Al-Waili ◽

...

Keyword(s):

Wound Healing ◽

Mouse Model ◽

Topical Application ◽

Marked Reduction ◽

Diabetic Mouse ◽

Type I ◽

Diabetic Mice ◽

Tnf Α ◽

Diabetic Wounds ◽

Tgf Β1

Background/Aims: Impaired wound healing is considered to be one of the most serious complications associated with diabetes as it significantly increases the susceptibility of patients to infection. Propolis is a natural bee product used extensively in foods and beverages that has significant benefits to human health. In particular, propolis has antioxidant, anti-inflammatory and analgesic effects that could be useful for improving wound healing. In this study, we investigated the effects of topical application of propolis on the healing and closure of diabetic wounds in a streptozotocin (STZ)-induced type I diabetic mouse model. Methods: Sixty male mice were distributed equally into 3 experimental groups: group 1, non-diabetic control mice; group 2, diabetic mice; and group 3, diabetic mice treated daily with a topical application of propolis. Results: We found that diabetic mice exhibited delayed wound closure characterized by a significant decrease in the levels of TGF-β1 and a prolonged elevation of the levels of inflammatory cytokines (IL-1β, IL-6 and TNF-α) and MMP9 in wound tissues compared with control non-diabetic mice. Moreover, the wound tissues of diabetic mice showed a marked reduction in the phosphorylation of Smad2 and Smad3 as well as a marked reduction in collagen production. Interestingly, compared with untreated diabetic mice, topical application of propolis significantly enhanced the closure of diabetic wounds and decreased the levels of IL-1β, IL-6, TNF-α and MMP9 to near normal levels. Most importantly, compared with untreated diabetic mice, the treatment of diabetic mice with propolis significantly enhanced the production of collagen via the TGF-β1/Smad2,3 signaling axis in wounded tissues. Conclusion: Our findings reveal the molecular mechanisms underlying the improved healing and closure of diabetic wounds following topical propolis application.

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Sodium Butyrate Plus EGF and PDGF-BB Aids Cutaneous Wound Healing in Diabetic Mice

Advances in Biology ◽

10.1155/2015/527231 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10

Author(s):

Rohini Keshava ◽

Rajalakshmi Gope

Keyword(s):

Wound Healing ◽

Growth Factors ◽

Topical Application ◽

Sodium Butyrate ◽

Diabetic Patients ◽

Cutaneous Wound Healing ◽

Diabetic Mice ◽

Tissue Samples ◽

Cutaneous Wound ◽

Diabetic Wounds

Topical application of growth factors is known to aid defective and/or delayed wound healing in diabetic patients. In this study, the effect of topical application of sodium butyrate (Na-Bu), EGF, and PDGF-BB was analysed on the acute cutaneous wound healing in streptozotocin (STZ) induced diabetic mouse model. Two cutaneous wounds were created, one on each of the dorsolateral sides of the diabetic mice. Na-Bu, EGF, and PDGF-BB were applied to the wound either individually or in various combinations. The wound healing was monitored visually and scored as percentage wound closure. The tissue samples were collected from the wound site at 1, 7, and 14 days after wounding from the treated and untreated diabetic wounds and analysed for the levels of EGF-R, β-PDGF-R, HDAC1, p21, and phosphorylated and hypophosphorylated pRb proteins. Our results indicate that application of EGF plus PDGF-BB at the initial stages followed by subsequent addition of Na-Bu along with these growth factors helps wound healing in diabetic mice. It appears that, in addition to cell proliferative agents, a cell differentiation agent, Na-Bu, is necessary for diabetic wound healing. Topical application of EGF plus PDGF-BB along with Na-Bu could be developed as therapeutic agents to treat and manage human diabetic wounds.

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