Phytochemical analysis with the antioxidant and aldose reductase inhibitory capacities of Tephrosia humilis aerial parts’ extracts

The genus Phyllanthus (family Euphorbiaceae) is considered one of the important medicinal and ornamental plants. A phytochemical analysis of the extracts was performed to search for the active ingredient. Results of the investigation of the hepatoprotective activity of Phyllanthus atropurpureus Boj. Hort. Maurit. revealed that the activities of alcoholic extracts of its aerial parts and roots were quite similar to those of silymarin. Both of them improve the parameters of CCl4-induced liver injury including serum aspartate aminotransferase and alanine aminotransferase. Among the extracts tested, the root extract showed maximum activity compared to the aerial parts extract and to silymarin.

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Phytochemical Analysis of Podospermum and Scorzonera n-Hexane Extracts and the HPLC Quantitation of Triterpenes

Molecules ◽

10.3390/molecules23071813 ◽

2018 ◽

Vol 23 (7) ◽

pp. 1813 ◽

Cited By ~ 4

Author(s):

Özlem Bahadır-Acıkara ◽

Serkan Özbilgin ◽

Gülcin Saltan-İşcan ◽

Stefano Dall’Acqua ◽

Veronika Rjašková ◽

...

Keyword(s):

Hplc Method ◽

Phytochemical Analysis ◽

Qualitative And Quantitative Analysis ◽

Qualitative And Quantitative ◽

Aerial Parts ◽

Hexane Extracts ◽

Lupeol Acetate ◽

Anti Inflammatory

Previously tested n-hexane extracts of the Scorzonera latifolia showed promising bioactivity in vivo. Because triterpenes could account for this activity, n-hexane extracts were analyzed by HPLC to identify and quantify the triterpenes as the most abundant constituents. Other Scorzonera and Podospermum species, potentially containing triterpenic aglycones, were included in the study. An HPLC method for simultaneous determination of triterpene aglycones was therefore developed for analysis of Podospermum and Scorzonera species. n-Hexane extracts of root and aerial parts of S. latifolia, ten other Scorzonera species and two Podospermum species were studied to compare the content of triterpenes. HPLC was used for the qualitative and quantitative analysis of α-amyrin, lupeol, lupeol acetate, taraxasteryl acetate, 3-β-hydroxy-fern-7-en-6-one acetate, urs-12-en-11-one-3-acetyl, 3-β-hydroxy-fern-8-en-7-one acetate, and olean-12-en-11-one-3-acetyl. Limits of detection and quantification were determined for each compound. HPLC fingerprinting of n-hexane extracts of Podospermum and Scorzonera species revealed relatively large amounts of triterpenes in a majority of investigated taxa. Lupeol, lupeol acetate, and taraxasteryl acetate were found in a majority of the species, except S. acuminata. The presence of α-amyrin, 3β-hydroxy-fern-7-en-6-one-acetate, urs-12-en-11-one-3-acetyl, 3β-hydroxy-fern-8-en-7-one-acetate, and olean-12-en-11-one-3-acetyl was detected in varying amounts. The triterpene content could correlate with the analgesic and anti-inflammatory activity of Scorzonera, which was previously observed and Scorzonera species that have been determined to contain triterpenes in large amounts and have not yet been tested for their analgesic activity should be tested for their potential analgesic and anti-inflammatory potential. The presented HPLC method can be used for analysis of triterpene aglycones, for example dedicated to chemosystematic studies of the Scorzonerinae.

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A PHYTOCHEMICAL STUDY ON EUPATORIUM GLANDULOSUM

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i1.29534 ◽

2019 ◽

pp. 77-80

Author(s):

SILPA M ◽

SURESH JOGHEE ◽

HAMSALAKSHMI

Keyword(s):

Ethyl Acetate ◽

Water Soluble ◽

Phytochemical Analysis ◽

Flavonoid Content ◽

Plant Materials ◽

Phytochemical Study ◽

Aerial Parts ◽

Standard Procedures ◽

Soxhlet Method ◽

Alcohol Ethyl

Objective: Eupatorium glandulosum is a useful medicinal plant belongs to the family Asteraceae. It is traditionally used to treat various diseases such as wound healing, antioxidant, and antiproliferative. The present study was aimed to investigate the physicochemical and phytochemical properties of various extracts of aerial parts of E. glandulosum. Methods: The E. glandulosum plant materials were extracted using the solvents alcohol, ethyl acetate, and chloroform by Soxhlet method. The extracts were screened for physiochemical constants, preliminary phytochemical analysis for carbohydrates, glycosides, alkaloids, flavonoids, phenols, tannins, and saponins. The quantitative phytochemical analysis was carried out for total flavonoid and total phenols using standard procedures. Results: The physiochemical constituents such as total ash, acid-insoluble ash, and water-soluble ash were found to be 14.25% (w/w), 5% (w/w), and 7.30% (w/w), respectively. The preliminary phytochemical screening confirmed the presence of carbohydrates, glycosides, alkaloids, flavonoids, phenols, tannins, and saponins. The flavonoid content of the plant extracts was found to be in the descending order ethyl acetate ˃alcohol ˃chloroform and the phenolic content was found to be alcohol ˃ethyl acetate ˃chloroform. Conclusion: The result showed the presence of phytochemical constituents and higher values of phenolic and flavonoid content make the plant useful for the formulation of the different drugs for human uses for treating various diseases.

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Rindera graeca (Boraginaceae) Phytochemical Profile and Biological Activities

Molecules ◽

10.3390/molecules25163625 ◽

2020 ◽

Vol 25 (16) ◽

pp. 3625

Author(s):

Christos Ganos ◽

Nektarios Aligiannis ◽

Ioanna Chinou ◽

Nikolaos Naziris ◽

Maria Chountoulesi ◽

...

Keyword(s):

Free Radical ◽

Biological Activities ◽

Biological Membrane ◽

3D Structure ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Endemic Plant ◽

Aerial Parts ◽

Potential Applications ◽

First Time

Rindera graeca is a Greek endemic plant of the Boraginaceae family which has never been studied before. Consequently, this study attempted to phytochemically examine the aerial parts of this species. Nine phenolic secondary metabolites were identified, consisting of seven caffeic acid derivatives and two flavonol glucosides, namely rutin and quercetin-3-rutinoside-7-rhamnoside. These flavonoids, together with rosmarinic acid, were isolated via column chromatography and structurally determined through spectral analysis. Quercetin-3-rutinoside-7-rhamnoside is an unusual triglycoside, which is identified for the first time in Rindera genus and among Boraginaceae plants. This metabolite was further examined with thermal analysis and its 3D structure was simulated, revealing some intriguing information on its interaction with biological membrane models, which might have potential applications in microcirculation-related conditions. R. graeca was also analyzed for its pyrrolizidine alkaloids content, and it was found to contain echinatine together with echinatine N-oxide and rinderine N-oxide. Additionally, the total phenolic and flavonoid contents of R. graeca methanol extract were determined, along with free radical inhibition assays. High total phenolic content and almost complete inhibition at experimental doses at the free radical assays indicate a potent antioxidant profile for this plant. Overall, through phytochemical analysis and biological activity assays, insight was gained on an endemic Greek species of the little-studied Rindera genus, while its potential for further applications has been assessed.

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Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

Molecules ◽

10.3390/molecules23020463 ◽

2018 ◽

Vol 23 (2) ◽

pp. 463 ◽

Cited By ~ 12

Author(s):

Nassima Boutaoui ◽

Lahcene Zaiter ◽

Fadila Benayache ◽

Samir Benayache ◽

Simone Carradori ◽

...

Keyword(s):

Phytochemical Analysis ◽

Qualitative And Quantitative ◽

Thymus Algeriensis ◽

Aerial Parts

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Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of Smilax larvata (Smilacaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/9894610 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 3

Author(s):

Beatriz Cristina Konopatzki Hirota ◽

Cristiane da Silva Paula ◽

Vinícius Bednarczuk de Oliveira ◽

Joice Maria da Cunha ◽

Anne Karoline Schreiber ◽

...

Keyword(s):

Antinociceptive Effect ◽

Opioid Antagonist ◽

In Vitro Assays ◽

Mustard Oil ◽

Phytochemical Analysis ◽

Etoac Extract ◽

Aerial Parts ◽

Nociceptive Responses ◽

Anti Inflammatory

The tea of aerial parts of Smilax larvata Griseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts of S. larvata were phytochemically and pharmacologically characterized. The phytochemical analysis of EtOAc extract of S. larvata revealed the presence of three flavonoids, drabanemoroside, kaempferol 3-O-α-L-rhamnopyranosyl(1→2)-α-L-rhamnopyranoside, and kaempferol, the first two being isolated for the first time in this genus, two phenolic compounds p-hydroxybenzoic acid and p-coumaric acid, and alkaloids. In vitro assays demonstrated a potential antioxidant property of SLG. The treatment with SLG induced a significant reduction of the formalin-evoked flinches in rats, an effect reversed by opioid antagonist naloxone. Treatment with SLG also induced a significant increase in the hot plate latency and a decrease of intestinal motility by 45%. No effect was observed over nociceptive responses induced by a TRPA1 agonist mustard oil or over acetic acid-induced writhing in mice. Together, our data suggested that SLG has an in vivo antinociceptive effect, which seems to be associated with the opioid system activation. These findings support previous claims of medical use of Smilax larvata in the treatment of pain conditions.

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Evaluation of antinociceptive, antinflammatory activities and phytochemical analysis of aerial parts of Urtica urens L.

Phytotherapy Research ◽

10.1002/ptr.3188 ◽

2010 ◽

Vol 24 (12) ◽

pp. 1807-1812 ◽

Cited By ~ 33

Author(s):

Carla Marrassini ◽

Cristina Acevedo ◽

Jorge Miño ◽

Graciela Ferraro ◽

Susana Gorzalczany

Keyword(s):

Phytochemical Analysis ◽

Aerial Parts ◽

Urtica Urens

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Evaluation of In Vitro Anti-Oxidant Activity of Mentha arvensis Linn in Memory Enhancing Study

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/phyto.v9i08.9627 ◽

2017 ◽

Vol 9 (08) ◽

Author(s):

Nazima Ameen ◽

Sabeeha Shafi

Keyword(s):

Heart Disease ◽

Ischemic Heart Disease ◽

Parkinson Disease ◽

Ethanolic Extract ◽

Phytochemical Analysis ◽

Mentha Arvensis ◽

Aerial Parts ◽

Anti Oxidant ◽

Memory Enhancing

The main objective of the study was to evaluate the in vitro anti-oxidant parameters for the study of memory enhancing activity of Mentha arvensis Linn closely related to Alzheimers disease. Mentha arvensisLinn is a plant commonly known as Pudina.It is used in our daily cuisines in India. The study included the aerial parts of Mentha arvensis belonging to family Lamiaceae. Two extracts were taken the aqueous and ethanolic extract. The phytochemical analysis of plant of Mentha arvensis Linn showed the presence of alkaloids, tannins,saponins, glycosides, terpenes, flavonoids, fats, carbohydrates, steroids and proteins.Oxidative compounds are responsible for causing many diseases like ischemic heart disease, Parkinson disease, hyperlipidemia, atherosclerosis and Alzhemers disease. In order to cure these diseases anti-oxidants are used. Keeping these facts in view, the anti-oxidant activity of Mentha arvensis Linn was evaluated in memory enhancing activity.

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