Determination of acetylcholinesterase and α-glucosidase inhibition by electrophoretically-mediated microanalysis and phenolic profile by HPLC-ESI-MS/MS of fruit juices from Brazilian Myrtaceae Plinia cauliflora (Mart.) Kausel and Eugenia uniflora L.

2019 ◽  
Vol 34 (18) ◽  
pp. 2683-2688 ◽  
Author(s):  
Diogo Alexandre Siebert ◽  
Flávia de Mello ◽  
Michele Debiasi Alberton ◽  
Luciano Vitali ◽  
Gustavo Amadeu Micke
Keyword(s):  
Fruit Juices ◽  
Phenolic Profile ◽  
Eugenia Uniflora ◽  
Esi Ms ◽  
Electrophoretically Mediated Microanalysis

Determination of Glutamate and GABA Released by Mouse Embryonic Stem Cells Using HILIC-ESI-MS/MS

2020 ◽  
Vol 10 (2) ◽  
pp. 122-129
Author(s):  
Haoyu Lv ◽  
Yabin Tang ◽  
Fan Sun ◽  
Shimin An ◽  
Xinjie Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Reversed Phase ◽  
Water Soluble ◽  
Substitution Matrix ◽  
Neural Cells ◽  
Esi Ms

Background:In recent years, more and more researches have shown that neurotransmitters can also be synthesized and released by peripheral non-neural cells. However, specificity and high sensitivity detection means were required for confirming ESCs autocrine glutamate and γ - aminobutyric acid (GABA). Glutamate and GABA are water-soluble and polar compounds which cannot be retained on a reversed phase C18 column, and their contents are often at a trace level. On the other hand, the biological matrix such as cell culture fluid contains a large number of amino acids, vitamins, carbohydrates, inorganic ions and other substances. Therefore, the main problem is the selection of the chromatographic column to avoid matrix interference.Objective:To establish a rapid and reliable method for the simultaneous determination of glutamate and GABA released from embryonic stem cells based on analytical chemistry.Methods:Glutamate and GABA released from mouse embryonic stem cells were determined on the basis of hydrophilic interaction chromatography coupled with electrospray ionization tandem Mass Spectrometry (HILIC- ESI- MS/MS), using isotope internal standards and substitution matrix method.Results:Undifferentiated embryonic stem cells autocrine glutamate and GABA and will reach releasing- reuptacking dynamic equilibriums at different time points. In contrast, neither glutamate nor GABA releasing could be detected from the MEFs, indicating the specificity release of the mESCs in the applied analytic method.Conclusion:A novel, simple, sensitive, selective and quantitative method was developed for determination of the glutamate and GABA from mouse embryonic stem cells.

scholarly journals Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 153
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Ioannis Raptis ◽  
Panagiota Petrou ◽  
Evangelia Livaniou ◽  
...  
Keyword(s):  
White Light ◽  
Optical Biosensor ◽  
Reflectance Spectroscopy ◽  
Fruit Juices ◽  
Sample Treatment ◽  
Assay Sensitivity ◽  
Reliable Determination ◽  
Light Reflectance ◽  
Sensitive Determination

Carbendazim is a systemic benzimidazole-type fungicide with broad-spectrum activity against fungi that undermine food products safety and quality. Despite its effectiveness, carbendazim constitutes a major environmental pollutant, being hazardous to both humans and animals. Therefore, fast and reliable determination of carbendazim levels in water, soil, and food samples is of high importance for both food industry and public health. Herein, an optical biosensor based on white light reflectance spectroscopy (WLRS) for fast and sensitive determination of carbendazim in fruit juices is presented. The transducer is a Si/SiO2 chip functionalized with a benzimidazole conjugate, and determination is based on a competitive immunoassay format. Thus, for the assay, a mixture of an in-house developed rabbit polyclonal anti-carbendazim antibody with the standards or samples is pumped over the chip, followed by biotinylated secondary antibody and streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the Si/SiO2 chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is able to detect 20 ng/mL of carbendazim in fruit juices with high accuracy and precision (intra- and inter-assay CVs ≤ 6.9% and ≤9.4%, respectively) in less than 30 min, applying a simple sample treatment that alleviates any “matrix-effect” on the assay results and a 60 min preincubation step for improving assay sensitivity. Excellent analytical characteristics and short analysis time along with its small size render the proposed WLRS immunosensor ideal for future on-the-spot determination of carbendazim in food and environmental samples.

Simplified and rapid determination of Ca, K, Mg, and Na in fruit juices by flowing liquid cathode atmospheric glow discharge optical emission spectrometry

2021 ◽  
Author(s):  
Monika Gorska ◽  
Pawel Pohl
Keyword(s):  
Glow Discharge ◽  
Rapid Determination ◽  
Optical Emission ◽  
Fruit Juices ◽  
Emission Spectrometry ◽  
Atmospheric Pressure Glow Discharge ◽  
Flowing Liquid ◽  
Liquid Cathode ◽  
Pressure Glow Discharge

Atmospheric pressure glow discharge (APGD) microplasma, sustained between a flowing liquid cathode (FLC) and a tungsten anode, was applied for the determination of Ca, K, Mg, and Na in fruit juices with a simplified sample preparation procedure.

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