Detection of mobile colistin-resistance gene variants (mcr-1 and mcr-2) in urinary tract pathogens in Bangladesh: the last resort of infectious disease management colistin efficacy is under threat

2021 ◽  
Author(s):  
Bayasrin Ara ◽  
Umme Laila Urmi ◽  
Tanjum Ara Haque ◽  
Shamsun Nahar ◽  
Adity Rumnaz ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Urinary Tract ◽  
Disease Management ◽  
Resistance Gene ◽  
Gene Variants ◽  
Colistin Resistance

scholarly journals Genome sequences of two clinical Escherichia coli isolates harboring the novel colistin-resistance gene variants mcr-1.26 and mcr-1.27

Gut Pathogens ◽  
2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Bernd Neumann ◽  
Wiebke Rackwitz ◽  
Klaus-Peter Hunfeld ◽  
Stephan Fuchs ◽  
Guido Werner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Gene ◽  
Gene Variants ◽  
The Novel ◽  
Colistin Resistance ◽  
Genome Sequences

scholarly journals Characterisation of mobile colistin resistance genes (mcr-3 and mcr-5) in river and storm water in regions of the Western Cape of South Africa

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Yolandi Snyman ◽  
Andrew C. Whitelaw ◽  
Jo M. Barnes ◽  
Motlatji R. B. Maloba ◽  
Mae Newton-Foot
Keyword(s):  
South Africa ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Water Sources ◽  
Storm Water ◽  
Gene Variants ◽  
Western Cape ◽  
Colistin Resistance ◽  
Three Rivers

Abstract Background Colistin is regarded as a last-resort antimicrobial against multi-drug resistant Gram-negative bacteria (GNB), therefore the dissemination of colistin resistance in the environment is of great concern. Horizontal transfer of mobile colistin resistance (mcr) genes to potential pathogens poses a serious problem. This study aimed to describe the presence of colistin resistant GNB and mcr genes in river and storm water in regions of the Western Cape. Methods Water samples were collected from three rivers during May 2019 and January 2020 and two storm water samples were collected in November 2019. Colistin resistant GNB were cultured on MacConkey agar containing colistin and identified by MALDI-TOF. Colistin resistance was confirmed using broth microdilution (BMD). mcr-1-5 genes were detected by PCR performed directly on the water samples and on the colistin resistant isolates. mcr functionality was assessed by BMD after cloning the mcr genes into pET-48b(+) and expression in SHuffle T7 E. coli. Results mcr-5.1 and various mcr-3 gene variants were detected in the Plankenburg-, Eerste- and Berg rivers and in storm water from Muizenberg, and only mcr-5.1 was detected in storm water from Fish Hoek. Colistin resistant GNB were isolated from all of the water sources. Aeromonas spp. were the most common colistin resistant organisms detected in the water sources; 25% (6/24) of colistin resistant Aeromonas spp. isolated from the Berg river contained novel mcr-3 variants; mcr-3.33 (n = 1), mcr-3.34 (n = 1) mcr-3.35 (n = 1) mcr-3.36 (n = 2) and mcr-3.37 (n = 1), which were confirmed to confer colistin resistance. Conclusions The mcr-5.1 and mcr-3 colistin resistance gene variants were present in widely dispersed water sources in regions of the Western Cape. The mcr genes were only detected in water sampled downstream of and alongside communities, suggesting that their presence is driven by human influence/contamination. This is the first documentation of mcr-3 and mcr-5 gene variants in any setting in South Africa. Spill-over of these genes to communities could result in horizontal gene transfer to pathogenic bacteria, exacerbating the challenge of controlling multidrug resistant GNB infections.

scholarly journals Addressing the growing burden of non–communicable disease by leveraging lessons from infectious disease management

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Peter Piot ◽  
Aya Caldwell ◽  
Peter Lamptey ◽  
Moffat Nyrirenda ◽  
Sunil Mehra ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Disease Management ◽  
Communicable Disease ◽  
Non Communicable Disease

scholarly journals The global distribution and spread of the mobilized colistin resistance gene mcr-1

2017 ◽  
Author(s):  
Ruobing Wang ◽  
Lucy van Dorp ◽  
Liam Shaw ◽  
Phelim Bradley ◽  
Qi Wang ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Global Distribution ◽  
Colistin Resistance ◽  
Posterior Density ◽  
Carbapenem Resistant ◽  
Significant Public Health ◽  
Monitoring And Surveillance ◽  
Multiple Levels ◽  
Carbapenem Resistant Enterobacteriaceae

AbstractColistin represents one of the very few available drugs for treating infections caused by carbapenem resistant Enterobacteriaceae (CRE). As such, the recent plasmid-mediated spread of the mobilized colistin resistance gene mcr-1 poses a significant public health threat requiring global monitoring and surveillance. In this work, we characterize the global distribution of mcr-1 using a dataset of 457 mcr-1 positive sequenced isolates consisting of currently publicly available mcr-1 carrying sequences combined with an additional 110 newly sequenced mcr-1 positive isolates from China. We find mcr-1 in a diversity of plasmid backgrounds but identify an immediate background common to all mcr-1 sequences. Our analyses establish that all mcr-1 elements in circulation descend from the same initial mobilization of mcr-1 by an ISApl1 transposon in the mid 2000s (2002-2008; 95% higher posterior density), followed by a dramatic demographic expansion, which led to its current global distribution. Our results provide the first systematic phylogenetic analysis of the origin and spread of mcr-1, and emphasize the importance of understanding the movement of mobile elements carrying antibiotic resistance genes across multiple levels of genomic organization.

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