Thermostable xylanase production and partial purification by solid-state fermentation using agricultural waste wheat straw

Two thermophilic fungi, Thermomyces lanuginosus BPJ-10 and Rhizomucor pusillus BPJ-2 were studied under solid state fermentation (SSF) using wheat bran for the production of thermostable xylanase. The optimum time required for the production of xylanase was found to be 4 days and 7 days for R. pusillus BPJ-2 and T. lanuginosus BPJ-10 respectively. The optimum temperatures for the production of xylanase by R. pusillus BPJ-2 and T. lanuginosus BPJ-10 were 45°C and 50°C respectively. The maximum activity of xylanase (1.685 IU/ml and 0.075 IU/ml) was exhibited by T. lanuginosus BPJ-10 and R. pusillus BPJ-2 at pH 7.0 and pH 4.0 respectively. The optimum moisture content for maximum xylanase production was 90% for both fungi. J. Asiat. Soc. Bangladesh, Sci. 39(1): 43-51, June 2013 DOI: http://dx.doi.org/10.3329/jasbs.v39i1.16032

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Xylanase production using agro-residue in solid-state fermentation from Bacillus pumilus ASH for biodelignification of wheat straw pulp

Biodegradation ◽

10.1007/s10532-011-9470-4 ◽

2011 ◽

Vol 22 (6) ◽

pp. 1143-1154 ◽

Cited By ~ 14

Author(s):

Gaurav Garg ◽

Ritu Mahajan ◽

Amanjot Kaur ◽

Jitender Sharma

Keyword(s):

Solid State ◽

Wheat Straw ◽

Solid State Fermentation ◽

Bacillus Pumilus ◽

Xylanase Production ◽

Wheat Straw Pulp

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Statistical Optimization of Media Components for Xylanase Production by Aspergillus spp. Using Solid State Fermentation and its Application in Fruit Juice Clarification

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i54a33733 ◽

2021 ◽

pp. 151-166

Author(s):

Laxmikant R. Patil ◽

Anil R. Shet ◽

Sharanappa Achappa ◽

Shivalingsarj V. Desai ◽

Veeranna S. Hombalimath ◽

...

Keyword(s):

Solid State ◽

Yeast Extract ◽

Wheat Bran ◽

Solid State Fermentation ◽

Carbon Sources ◽

Gel Filtration ◽

Partial Purification ◽

Elution Time ◽

Xylanase Production ◽

Juice Clarification

Xylanases are enzymes that convert xylan into xylose, xylobiose, and xylotriose. The present study deals with the production and optimization of xylanase through Solid-State Fermentation (SSF) using different agricultural wastes by Aspergillus spp. The Plackett Burman (PB) design was used to screen significant media components affecting the xylanase production. The carbon sources screened were wheat bran, rice bran, sugarcane bagasse, corn cob, and orange peel. The nitrogen sources screened were yeast extract, peptone, (NH4)2SO4, Na2NO3, and urea. Also, nine different salts such as KCl, MgSO4, Na2HPO4, CaCl2, FeSO4, ZnSO4, Na2CO3, KH2PO4, and NaH2PO4 which act as trace elements were screened. The results showed that wheat bran, yeast extract, Na2NO3 and KCl are the significant factors that affect xylanase production. A 33 Full Factorial Design (FFD) was performed to optimize the significant media components (wheat bran, KCl, yeast extract) obtained from PB design using Response Surface Methodology (RSM). Statistical analysis of results showed that wheat bran, KCl, yeast extract, and interaction between wheat bran and yeast extract were found to be significant. The optimum concentration of wheat bran, KCl, yeast extract was 8 g/L, 0.1 g/L and 3 g/L. The Partial purification of xylanase was carried out using ammonium salt precipitation and dialysis. Gel filtration chromatography was performed to optimize the elution time, which was found to be 6 minutes. Application of xylanase in orange juice clarification was studied at 40 °C, 50 °C, and 60 °C. The optimum temperature obtained was 60 ºC.

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Optimization of Xylanase Production from Aspergillus Flavus in Solid State Fermentation Using Agricultural Waste as a Substrate

International Journal of Advances in Interdisciplinary Research ◽

10.29191/ijaidr.2017.4.3.06 ◽

2017 ◽

Vol 4 (3) ◽

pp. 29 ◽

Cited By ~ 1

Author(s):

Tamilmani Periasamy ◽

Kalaivani Aiyasamy ◽

Margret Rosaland Fathima Mary George ◽

Thirumalaisamy Rathinavel ◽

...

Keyword(s):

Solid State ◽

Aspergillus Flavus ◽

Solid State Fermentation ◽

Agricultural Waste ◽

Xylanase Production

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Optimization of the simultaneous production of cellulase and xylanase by submerged and solid-state fermentation of wheat chaff

Journal of the Serbian Chemical Society ◽

10.2298/jsc190530080j ◽

2020 ◽

Vol 85 (2) ◽

pp. 177-189 ◽

Cited By ~ 1

Author(s):

Mirjana Jovanovic ◽

Damjan Vucurovic ◽

Bojana Bajic ◽

Sinisa Dodic ◽

Vanja Vlajkov ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Submerged Fermentation ◽

Ph Value ◽

Agricultural Waste ◽

Hydrolytic Enzymes ◽

Raw Material ◽

Optimal Conditions ◽

Xylanase Production ◽

Wheat Chaff

Wheat chaff as an agricultural waste represents a cheap raw material for biotechnological processes. With its lignocellulosic composition, it is suitable for producing hydrolytic enzymes for second generation renewable fuel production technologies. The aim of this work was to optimize the process parameters (cultivation temperature 25?35?C, pH value 4?6 and cultivation time 3?7 days) of the cultivating fungi (Trichoderma reesei QM 9414) on a media based on wheat chaff by submerged and solid-state techniques, in order to enhance and compare the two types of simultaneous cellulase and xylanase production. Optimal conditions for the submerged fermentation were 29.65?C for temperature, pH 4.27 and 7 days of cultivation, while for the solid-state fermentation, the optimal conditions were 28.01?C, pH 6.00 and 7 days. The cellulolytic and xylanolytic activities of the obtained cultivation broth filtrates were 0.0535 and 0.1676 U mL-1 for the submerged fermentation, and 0.0407 and 0.1401 U mL-1 for the solid-state fermentation, respectively, and with a 26.77 and 13.39 % enhancement of enzyme activity for submerged fermentation, and a 22.96 and 42.66 % enhancement for solid-state fermentation, respectively, compared to the results obtained before optimization.

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Study on the Solid-State Fermentation Conditions for Producing Thermostable Xylanase Feed in a Pressure Pulsation Bioreactor

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.72 ◽

2011 ◽

Vol 236-238 ◽

pp. 72-76

Author(s):

Ge Yang ◽

Li Li Hou ◽

Fu Liang Zhang

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Enzyme Production ◽

Xylanase Activity ◽

Air Pressure ◽

Thermomyces Lanuginosus ◽

Pressure Amplitude ◽

Thermostable Xylanase ◽

Xylanase Production ◽

Periodic Pressure

Air pressure amplitude serves as a critical control parameter in periodic pressure solid state fermentation process. Effects of air pressure amplitudes on thermostable xylanase production byThermomyces lanuginosusSD-21 were investigated. Under the optimum periodic pressure amplitude, namely: at lower limit of 0.05 MPa and upper limit of 1.5 MPa. Among the lignocellulosic substrates tested, corn cob and wheat bran supported a high xylanase (EC 3.2.1.8) secretion byHumicola lanuginosain solid-state fermentation (SSF). Enzyme production reached a peak in 96 h followed by a decline thereafter. Enzyme production was very high, xylanase activity 8237 IU /g of dry moldy bran can be obtained in the system compared with 4520 IU/g in conventional tray fermenter, cultivation of the mold in large enamel trays yielded a xylanase titer comparable with that in flasks. Parametric optimization resulted in a 45.13% increase in enzyme production in PPSSF.

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