scholarly journals The peculiarities of piRNA expression upon heat shock exposure inDrosophila melanogaster

2015 ◽  
Vol 5 (5) ◽  
pp. 72-80 ◽  
Author(s):  
S Yu Funikov ◽  
SS Ryazansky ◽  
ES Zelentsova ◽  
VI Popenko ◽  
OG Leonova ◽  
...  
Keyword(s):  
2009 ◽  
Vol 407 (7) ◽  
pp. 2411-2419 ◽  
Author(s):  
Shu-jun Xu ◽  
Chun-jiang Liu ◽  
Ping-an Jiang ◽  
Wei-min Cai ◽  
Yan Wang

2000 ◽  
Vol 23 (2) ◽  
pp. 301-304 ◽  
Author(s):  
Maria Luiza S. Mello ◽  
Sílvya S. Maria ◽  
Maria Cristina H. Tavares

The survival of Triatoma infestans and of somatic cells from this species is affected by heat shock. In this study, we examined the cell death responses of male germ line cells from 5th instar nymphs 7 and 30 days after heat shock exposure (40ºC, 1 h). The preparations were stained by the Feulgen reaction and the TUNEL immunocytochemical assay. Apoptosis was elicited by heat shock, with positive TUNEL responses in spermatogonial chromatin and chromosomes, spermatids and sperm cells. Spermatogonia were most affected seven days after the shock whereas some spermatids and sperm cells exhibited DNA fragmentation only thirty days after heat shock. The rate of cell death varied among the cells. In some cases, cellular differentiation was unaffected by heat shock, with DNA fragmentation occurring towards the end of spermatogenesis.


2019 ◽  
Vol 9 (2) ◽  
pp. 90-101
Author(s):  
Osama Galal Sakr ◽  
Baliegh Hamdy Mousa ◽  
Khamis Refaay Said Emam ◽  
Ali Saber Morsy ◽  
Nagwa Abd El-Hady Ahmed

2013 ◽  
Vol 12 (2) ◽  
pp. 653-660 ◽  
Author(s):  
Malgorzata Lagisz ◽  
Katie L. Hector ◽  
Shinichi Nakagawa

Author(s):  
Li-Chu Tung ◽  
Yung-Reui Chen ◽  
Shiu-Nan Chen ◽  
Guang-Hsiung Kuo

In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli, under heat shock treatment are described.The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV.


Sign in / Sign up

Export Citation Format

Share Document