Fish kidney cell line in response to heat shock

In the present study, the ultrastructural changes of BPK cells, a fibroblast-like cell line, derived from the kidney of juvenile black porgy Acanthopagrus schlegeli, under heat shock treatment are described.The BPK cells were maintained in L-15 medium supplemented with 10% fetal calf serum and 0.15 M NaCl at 28|C2. The heating was carried out in precalibrated water baths. Monolayers of cells, grown on coverslips in parafilm-sealed petri dishes were submerged under water for 30 min at 40|C treatments. Cells were fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer supplemented with 6.6% sucrose, postfixed in 1% OsO4 and flat embedded in Spurr’s resin. Silver section were cut parallel to the substratum, stained with uranyl acetate and Reynold’s lead citrate, and examined in a Hitachi H-600 electron microscope at 75 KV.

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Ultrastructural changes in sciatic nerve tissue: Effects of passive maternal smoking

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100076858 ◽

1983 ◽

Vol 41 ◽

pp. 650-651

Author(s):

Amankwah K.S. ◽

A.D. Weberg ◽

R.C. Kaufmann

Keyword(s):

Sciatic Nerve ◽

Maternal Smoking ◽

Ultrastructural Level ◽

Uranyl Acetate ◽

Nerve Tissue ◽

Ultrastructural Changes ◽

Cacodylate Buffer ◽

And Control ◽

Spontaneous Delivery

Previous research has revealed that passive (involuntary inhalation) tobacco smoking during gestation can have adverse effects upon the developing fetus. These prior investigations did not concentrate on changes in fetal morphology. This study was undertaken to delineate fetal neural abnormalities at the ultrastructural level in mice pups exposed in utero to passive maternal smoking.Pregnant study animals, housed in a special chamber, were subjected to cigarette smoke daily from conception until delivery. Blood tests for determination of carbon monoxide levels were run at 15-18 days gestation. Sciatic nerve tissue from experimental and control animals were obtained following spontaneous delivery and fixed in 2.5% gluteraldehyde in 0.1M cacodylate buffer pH 7.3. The samples were post-fixed in osmium ferrocyanide (1:1 mixture of 1.5% aqueous OSO4 and 2.5% K4 Fe(CN)6). Following dehydration, the tissues were infiltrated with and embedded in Spurr. Sections were stained with uranyl acetate and lead citrate.

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Fetal calf serum and retinoic acid affect proliferation and terminal differentiation of a rat rhabdomyosarcoma cell line (BA-HAN-1C)

British Journal of Cancer ◽

10.1038/bjc.1989.12 ◽

1989 ◽

Vol 59 (1) ◽

pp. 61-67 ◽

Cited By ~ 9

Author(s):

CD Gerharz ◽

HE Gabbert ◽

HK Biesalski ◽

R Engers ◽

C Luley

Keyword(s):

Retinoic Acid ◽

Cell Line ◽

Fetal Calf Serum ◽

Calf Serum ◽

Terminal Differentiation ◽

Rhabdomyosarcoma Cell Line ◽

Rhabdomyosarcoma Cell

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Interleukin 1 induction of a serine esterase in a murine T cell line is inhibited by fetal calf serum

Immunology Letters ◽

10.1016/0165-2478(89)90065-5 ◽

1989 ◽

Vol 20 (1) ◽

pp. 35-39 ◽

Cited By ~ 4

Author(s):

Susanna E. Schlegel-Haueter ◽

Florence Aebischer

Keyword(s):

T Cell ◽

Cell Line ◽

Fetal Calf Serum ◽

Interleukin 1 ◽

Calf Serum ◽

Serine Esterase

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Ultrastructure of the Synovial Microvasculature in Rheumatoid Arthritis (ra) and Systemic Lupus Erythematosus (SLE)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068199 ◽

1970 ◽

Vol 28 ◽

pp. 240-241

Author(s):

H. Ralph Schumacher

Keyword(s):

Lupus Erythematosus ◽

Uranyl Acetate ◽

Basement Membranes ◽

Ultrastructural Changes ◽

Lead Citrate ◽

Vascular Changes ◽

Systemic Lupus ◽

Degenerative Arthritis ◽

Cacodylate Buffer ◽

Human Joint

Synovial vascular alterations have been suggested to be important in the pathogenesis of both RA and SLE (1). Although vascular changes have been described by light microscopy the presence of significant ultrastructural changes has been questioned (2). This report describes an EM study of needle synovial biopsies from 5 patients each with classical RA and SLE. Specimens were promptly fixed in Karnovsky's paraformaldehyde-glutaraldehyde diluted 1:1 with 0.1 M cacodylate buffer at pH 7.4, washed in buffer, post-fixed in Palade's osmium-veronal, dehydrated with alcohol, embedded in epon, cut, and stained with lead citrate and uranyl acetate. Vascular changes were seen in both groups with some findings common to both diseases. Basement membranes were multilaminated. (Fig.l). This was not seen in normal rabbits and monkeys but was also present in other human joint diseases including degenerative arthritis. Venular endothelium was active appearing with filopodia extending into the lumen and with gaps demonstrable between endothelial cells (Fig.2,3). Platelets occluded some gaps (Fig.2).

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Ultrastructural Characteristics of Vaginal Epithelium of Persistent Estrous Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119983 ◽

1985 ◽

Vol 43 ◽

pp. 658-659

Author(s):

Khosho Francis K. ◽

Kaufmann Robert C. ◽

Amankwah Kofi S.

Keyword(s):

Osmium Tetroxide ◽

Uranyl Acetate ◽

Ruthenium Red ◽

Female Rats ◽

Constant Light ◽

Ultrastructural Changes ◽

Vaginal Smears ◽

Thin Sections ◽

Cacodylate Buffer ◽

Ultrastructural Characteristics

Adult female rats exposed to constant light will develop anovulatory acyclicity characterized by persistent vaginal cornification (PE) and formation of multiple large cystic follicles on the ovaries. The purpose of the present communication is to describe the ultrastructural changes in vaginal epithelia in PE rats as compared to that in normal estrous rats.Persistent vaginal estrous with PCO was induced in a group of Sprague-Dawely rats by exposure to constant light for 50-150 days. Rats in normal estrous, as determined by vaginal smears, were used as controls. Nembutal- anethesized rats were perfused through the aorta with 2.5% gluteraldehyde in 1M sodium cacodylate buffer (pH 7.3). The mucosa of the vaginal folds just inferior to the cervix were dissected by microsurgery, postfixed, stained with 0.5% ruthenium red in 1% osmium tetroxide, dehydrated, and embedded in polybed. Thick sections (1μ) were stained with toludine blue for light microscopy studies. Thin sections were stained with uranyl acetate and lead citrate.

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Production and Main Characteristics of a Fetal Calf Serum-specific Cell Line that Induces T and B Cell Differentiation

Scandinavian Journal of Immunology ◽

10.1111/j.1365-3083.1980.tb00084.x ◽

1980 ◽

Vol 12 (5) ◽

pp. 401-409 ◽

Cited By ~ 12

Author(s):

B. RUBIN ◽

M. A. COOLEY ◽

C. BORGNE DE KAOUEL ◽

R. B. TAYLOR ◽

P. GOLSTEIN

Keyword(s):

Cell Differentiation ◽

B Cell ◽

Cell Line ◽

Fetal Calf Serum ◽

Calf Serum ◽

Specific Cell ◽

B Cell Differentiation

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Mitochondrial impairment and recovery after heat shock treatment in a human microglial cell line

Neurochemistry International ◽

10.1016/s0197-0186(99)00118-7 ◽

2000 ◽

Vol 36 (3) ◽

pp. 233-241 ◽

Cited By ~ 17

Author(s):

Florence Macouillard-Poulletier de Gannes ◽

Nathalie Leducq ◽

Philippe Diolez ◽

Francis Belloc ◽

Michel Merle ◽

...

Keyword(s):

Heat Shock ◽

Cell Line ◽

Microglial Cell ◽

Shock Treatment ◽

Heat Shock Treatment ◽

Mitochondrial Impairment ◽

Microglial Cell Line

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An improved technique for visualization of Neisseria gonorrhoeae pili by SEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161540 ◽

1990 ◽

Vol 48 (3) ◽

pp. 796-797

Author(s):

Nusi P. Dekker ◽

Claudia J. Lammel ◽

Geo. F. Brooks

Keyword(s):

Neisseria Gonorrhoeae ◽

Culture Medium ◽

Endometrial Adenocarcinoma ◽

Calf Serum ◽

Uranyl Acetate ◽

Culture Cells ◽

Tissue Culture Cells ◽

Critical Point Drying ◽

Cacodylate Buffer ◽

Improved Technique

Piliated Neisseria gonorrhoeae (gc) are virulent and attach to human cells more readily than nonpiliated gc . Pili have been difficult to visualize in the scanning electron microscope (SEM), appearing as thick, branched structures, possibly because of artifacts or distortion associated with critical point drying (CPD) . The use of hexamethyldisilazane (HMDS), as an alternative to CPD with bacterial or tissue culture cells, as reported here, improved preservation and visualization of pili and other delicate surface structures.The methods were: Human endometrial adenocarcinoma (HEC-l-B, ATCC HTB 113) cells were grown in cell culture medium containing fetal calf serum on round thermanox coverslips. Suspensions of piliated and nonpiliated strains of gc (FA1090, F62-SF, and g1412) at dilutions of about 2 × 107 cfu were added to the cells and allowed to attach. Unattached gc were rinsed off and replaced with fresh media after 30 min. After 1 or 8h incubation, the cells were fixed in 2.5% gluteraldehyde in 0.1M cacodylate buffer for 4h; rinsed well in the buffer; post-fixed in 1% OsO4 in cacodylate buffer for lh; rinsed 3 × 10 min in buffer and 2 × 5 min in water; placed in 1% tannic acid in water for 30 min; rinsed 3 × 10 min in water; placed in 2% uranyl acetate for lh; rinsed 3 × 10 min in water; dehydrated in graded ethanols (50%, 70%, 95%, 2 × 5 min each; 100% 4 × 5 min; 100% 10 min); placed in HMDS for 10 min.

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