Integral membrane protein 2A enhances sensitivity to chemotherapy via notch signaling pathway in cervical cancer

Dynamic visualizations and expressive representations are needed in systems biology to handle multiple interactionsoccurring during the biological processes of biopathway representations. Dynamic visualizations allow users an easeof interaction with pathway models. At the same time, representations of biopathways should express how interactionstake place. In spite of the fact that diverse databases provide users with pathways, their information andrepresentation are frequently different from each other and show restricted interactions because of their staticvisualization. An adopted solution is to merge diverse representations to obtain a richer one. However, due to differentformats and the multiple links involved in the pathway representations, the merge results frequently in erroneousmodels and in a tangle web of relations very hard to be manipulated. Instead, this work introduces a concurrentdynamic visualization (CDV) of the same pathway, which is retrieved from different sites and then transformed intoPetri net representations to facilitate the understanding of their biological processes by interacting with them. Weapplied this approach to the analysis of the Notch signaling pathway, associated with cervical cancer; we obtained itfrom different sources which we compared and manipulated simultaneously by interacting with the provided CDV untilthe user generated a personalized pathway.

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Faculty Opinions recommendation of APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1003811.42959 ◽

2002 ◽

Author(s):

Matthew Freeman

Keyword(s):

Caenorhabditis Elegans ◽

Membrane Protein ◽

Notch Signaling ◽

Signaling Pathway ◽

Notch Signaling Pathway

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APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.022523499 ◽

2002 ◽

Vol 99 (2) ◽

pp. 775-779 ◽

Cited By ~ 288

Author(s):

C. Goutte ◽

M. Tsunozaki ◽

V. A. Hale ◽

J. R. Priess

Keyword(s):

Caenorhabditis Elegans ◽

Membrane Protein ◽

Notch Signaling ◽

Signaling Pathway ◽

Notch Signaling Pathway

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Rutin Mediated Apoptotic Cell Death in Caski Cervical Cancer Cells via Notch-1 and Hes-1 Downregulation

Life ◽

10.3390/life11080761 ◽

2021 ◽

Vol 11 (8) ◽

pp. 761

Author(s):

Fahad Khan ◽

Pratibha Pandey ◽

Niraj Kumar Jha ◽

Mohammad Khalid ◽

Shreesh Ojha

Keyword(s):

Cell Cycle ◽

Cervical Cancer ◽

Notch Signaling ◽

Mrna Expression ◽

Cancer Cells ◽

Signaling Pathway ◽

Cancer Therapeutics ◽

Notch Signaling Pathway ◽

Notch 1 ◽

Cervical Cancer Cells

Natural dietary molecules such as flavonoids have been recognized for their immense potential in cancer therapeutics with several health benefits. Hes-1 and Notch-1 overexpression has been associated with the progression of cervical cancer. However, the apoptosis-inducing potential of one such potent flavanol against these two key components of the Notch signaling pathway in cervical cancer has not been elucidated to date. Therefore, in this study, we performed several in vitro assays to gain detailed insight about the apoptotic inducing effect of rutin as well as its modulatory effect on Notch-1 and Hes-1 in cervical cancer cells. The results indicated that rutin led to a dose-dependent antiproliferative effects on Caski cervical cancer cells. DAPI and Mitotracker red staining revealed that rutin induced significant apoptotic effects via caspase-3/9 activation, ROS generation, and alteration in Bax/Bcl2 mRNA expression. Cell cycle analysis resulted in the arrest of cell cycle progression in G0/G1 that was associated with a reduced expression of CDK4 and Cyclin D1. The gene expression analysis further revealed that rutin treatment decreases Notch-1 and Hes-1 mRNA expression. Altogether, these results showed that rutin showed potent anticancer effects in human cervical cancer Caski cells by triggering apoptosis, G0/G1 phase arrest, and downregulating the level of Notch-1 and Hes-1 of the Notch signaling pathway.

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