The Prognostic Value of Plasma Complement Factor B (CFB) in Thyroid Carcinoma

The Prognostic Value of Plasma Complement Factor B (CFB) In Thyroid Carcinoma

10.21203/rs.3.rs-510321/v1 ◽

2021 ◽

Author(s):

Pu Wu ◽

Wei Sun ◽

Hao Zhang

Keyword(s):

Gene Expression ◽

T Cells ◽

Thyroid Carcinoma ◽

Immune Cells ◽

Cox Regression ◽

Enrichment Analysis ◽

Gene Set Enrichment Analysis ◽

Complement Factor ◽

Factor B ◽

Complement Factor B

Abstract Background: Stromal and immune cells are major components of tumor microenvironment (TME) and affect the growth and development of thyroid carcinoma (THCA). However, data on the exact mechanisms that define the relationship between the TME and THCA remains scant.Results: Here, we calculated stromal and immune cells scores and the proportion of tumor-infiltrating immune cells (TICs) by CIBERSORT and ESTIMATE based on the THCA gene expression data from The Cancer Genome Atlas database (TCGA). In addition, we evaluated differentially expressed genes (DEGs) from high- and low-score groups and then preformed Gene Ontology (GO) as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Besides, we carried out protein-protein interaction (PPI) analysis and COX regression on the DEGs. Our data showed a significant correlation between plasma complement factor B (CFB) and PTC development and prognosis. In addition, Gene Set Enrichment Analysis (GSEA) demonstrated that the CFB was mainly enriched in immune response pathways. Further dissection showed that the expression of CFB was positively correlated with T cells CD8, Macrophages M1, Plasma cells, T cells CD4 memory activated, T cells follicular helper and T cells regulatory (Tregs), whereas negatively correlated with Eosinophils, Macrophages M0, Macrophages M2, Mast cells resting, T cells CD4 memory resting in the TME. Finally, the expression level of CFB was verified by other cohorts form Gene Expression Omnibus (GEO) database. Conclusions: Taken together, our data demonstrated that the CFB could be a prognostic marker for THCA and its expression influences the infiltration of immune cells.

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Plasma protein expression profiles, cardiovascular disease, and religious struggles among South Asians in the MASALA study

Scientific Reports ◽

10.1038/s41598-020-79429-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Long H. Ngo ◽

M. Austin Argentieri ◽

Simon T. Dillon ◽

Blake Victor Kent ◽

Alka M. Kanaya ◽

...

Keyword(s):

Cardiovascular Disease ◽

Plasma Protein ◽

Expression Profiles ◽

South Asians ◽

Predictive Biomarkers ◽

Information Criteria ◽

Blood Protein ◽

Complement Factor ◽

Factor B ◽

Complement Factor B

AbstractBlood protein concentrations are clinically useful, predictive biomarkers of cardiovascular disease (CVD). Despite a higher burden of CVD among U.S. South Asians, no CVD-related proteomics study has been conducted in this sub-population. The aim of this study is to investigate the associations between plasma protein levels and CVD incidence, and to assess the potential influence of religiosity/spirituality (R/S) on significant protein-CVD associations, in South Asians from the MASALA Study. We used a nested case–control design of 50 participants with incident CVD and 50 sex- and age-matched controls. Plasma samples were analyzed by SOMAscan for expression of 1305 proteins. Multivariable logistic regression models and model selection using Akaike Information Criteria were performed on the proteins and clinical covariates, with further effect modification analyses conducted to assess the influence of R/S measures on significant associations between proteins and incident CVD events. We identified 36 proteins that were significantly expressed differentially among CVD cases compared to matched controls. These proteins are involved in immune cell recruitment, atherosclerosis, endothelial cell differentiation, and vascularization. A final multivariable model found three proteins (Contactin-5 [CNTN5], Low affinity immunoglobulin gamma Fc region receptor II-a [FCGR2A], and Complement factor B [CFB]) associated with incident CVD after adjustment for diabetes (AUC = 0.82). Religious struggles that exacerbate the adverse impact of stressful life events, significantly modified the effect of Contactin-5 and Complement factor B on risk of CVD. Our research is this first assessment of the relationship between protein concentrations and risk of CVD in a South Asian sample. Further research is needed to understand patterns of proteomic profiles across diverse ethnic communities, and the influence of resources for resiliency on proteomic signatures and ultimately, risk of CVD.

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