Effect of temperature-sensitive nanogel combined with angioplasty on sICAM-1 and VE-cadherin in lower extremity arterial occlusion rabbits

AbstractA key challenge to advance the efficiency of bioprocesses is the uncoupling of biomass from product formation, as biomass represents a by-product that is in most cases difficult to recycle efficiently. Using the example of rhamnolipid biosurfactants, a temperature-sensitive heterologous production system under translation control of a fourU RNA thermometer from Salmonella was established to allow separating phases of preferred growth from product formation. Rhamnolipids as bulk chemicals represent a model system for future processes of industrial biotechnology and are therefore tied to the efficiency requirements in competition with the chemical industry. Experimental data confirms function of the RNA thermometer and suggests a major effect of temperature on specific rhamnolipid production rates with an increase of the average production rate by a factor of 11 between 25 and 38 °C, while the major part of this increase is attributable to the regulatory effect of the RNA thermometer rather than an unspecific overall increase in bacterial metabolism. The production capacity of the developed temperature sensitive-system was evaluated in a simple batch process driven by a temperature switch. Product formation was evaluated by efficiency parameters and yields, confirming increased product formation rates and product-per-biomass yields compared to a high titer heterologous rhamnolipid production process from literature.

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Modelling tree ring cellulose <i>δ</i><sup>18</sup>O variations in two temperature-sensitive tree species from North and South America

Climate of the Past ◽

10.5194/cp-13-1515-2017 ◽

2017 ◽

Vol 13 (11) ◽

pp. 1515-1526 ◽

Cited By ~ 6

Author(s):

Aliénor Lavergne ◽

Fabio Gennaretti ◽

Camille Risi ◽

Valérie Daux ◽

Etienne Boucher ◽

...

Keyword(s):

South America ◽

Isotopic Fractionation ◽

Circulation Model ◽

Effect Of Temperature ◽

Temperature Sensitive ◽

Nothofagus Pumilio ◽

Climate Signal ◽

North And South America ◽

North And South ◽

Two Temperature

Abstract. Oxygen isotopes in tree rings (δ18OTR) are widely used to reconstruct past climates. However, the complexity of climatic and biological processes controlling isotopic fractionation is not yet fully understood. Here, we use the MAIDENiso model to decipher the variability in δ18OTR of two temperature-sensitive species of relevant palaeoclimatological interest (Picea mariana and Nothofagus pumilio) and growing at cold high latitudes in North and South America. In this first modelling study on δ18OTR values in both northeastern Canada (53.86° N) and western Argentina (41.10° S), we specifically aim at (1) evaluating the predictive skill of MAIDENiso to simulate δ18OTR values, (2) identifying the physical processes controlling δ18OTR by mechanistic modelling and (3) defining the origin of the temperature signal recorded in the two species. Although the linear regression models used here to predict daily δ18O of precipitation (δ18OP) may need to be improved in the future, the resulting daily δ18OP values adequately reproduce observed (from weather stations) and simulated (by global circulation model) δ18OP series. The δ18OTR values of the two species are correctly simulated using the δ18OP estimation as MAIDENiso input, although some offset in mean δ18OTR levels is observed for the South American site. For both species, the variability in δ18OTR series is primarily linked to the effect of temperature on isotopic enrichment of the leaf water. We show that MAIDENiso is a powerful tool for investigating isotopic fractionation processes but that the lack of a denser isotope-enabled monitoring network recording oxygen fractionation in the soil–vegetation–atmosphere compartments limits our capacity to decipher the processes at play. This study proves that the eco-physiological modelling of δ18OTR values is necessary to interpret the recorded climate signal more reliably.

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Understanding the effect of temperature on Bluetongue disease risk in livestock

10.1101/759860 ◽

2019 ◽

Cited By ~ 1

Author(s):

Fadoua El Moustaid ◽

Zorian Thronton ◽

Hani Slamani ◽

Sadie J. Ryan ◽

Leah R. Johnson

Keyword(s):

Disease Risk ◽

Vector Competence ◽

Basic Reproductive Number ◽

Effect Of Temperature ◽

Reproductive Number ◽

Temperature Sensitive ◽

Modeling Tools ◽

Vector Borne Diseases ◽

Thermal Range ◽

Vector Borne

AbstractThe transmission of vector-borne diseases is governed by complex factors including pathogen characteristics, vector-host interactions, and environmental conditions. Temperature is a major driver for many vector-borne diseases including Bluetongue viral (BTV) disease, a midge-borne febrile disease of ruminants, notably livestock, whose etiology ranges from mild or asymptomatic to rapidly fatal, thus threatening animal agriculture and the economy of affected countries. Using modeling tools, we seek to predict where transmission can occur based on suitable temperatures for BTV. We fit thermal performance curves to temperature sensitive midge life history traits, using a Bayesian approach. Then, we incorporated these into a new formula for the disease basic reproductive number, R0, to include trait responses, for two species of key midge vectors, Culicoides sonorensis and Culicoides variipennis. Our results show that outbreaks of BTV are more likely between 15°C and 33°C with predicted peak transmission at 26°C. The greatest uncertainty in R0 is associated with the uncertainty in: mortality and fecundity of midges near optimal temperature for transmission; midges’ probability of becoming infectious post infection at the lower edge of the thermal range; and the biting rate together with vector competence at the higher edge of the thermal range. We compare our R0 to two other R0 formulations and show that incorporating thermal curves into all three leads to similar BTV risk predictions. To demonstrate the utility of this model approach, we created global suitability maps indicating the areas at high and long-term risk of BTV transmission, to assess risk, and anticipate potential locations of establishment.

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Detection of Acute Arterial Occlusion During Artery Bypass Surgery for a Lower Extremity by Monitoring Regional Saturation of Oxygen of the Sole of the Foot

Journal of Cardiothoracic and Vascular Anesthesia ◽

10.1053/j.jvca.2017.03.003 ◽

2017 ◽

Vol 31 (6) ◽

pp. 2167-2169

Author(s):

Hiromichi Kurosaki ◽

Misako Higuchi ◽

Rika Nogawa ◽

Tomoyuki Kawamata

Keyword(s):

Lower Extremity ◽

Bypass Surgery ◽

Artery Bypass ◽

Arterial Occlusion ◽

Acute Arterial Occlusion ◽

Regional Saturation Of Oxygen ◽

Sole Of The Foot

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The effect of temperature on differentSalmonellaserotypes during warm seasons in a Mediterranean climate city, Adelaide, Australia

Epidemiology and Infection ◽

10.1017/s0950268815002587 ◽

2015 ◽

Vol 144 (6) ◽

pp. 1231-1240 ◽

Cited By ~ 19

Author(s):

A. MILAZZO ◽

L. C. GILES ◽

Y. ZHANG ◽

A. P. KOEHLER ◽

J. E. HILLER ◽

...

Keyword(s):

Phage Type ◽

Warm Season ◽

Substantial Effect ◽

Effect Of Temperature ◽

Temperature Sensitive ◽

Ambient Temperatures ◽

Policy Interventions ◽

Long Term Trends ◽

Higher Temperature ◽

The Impact

SUMMARYChanging trends in foodborne disease are influenced by many factors, including temperature. Globally and in Australia, warmer ambient temperatures are projected to rise if climate change continues.Salmonellaspp. are a temperature-sensitive pathogen and rising temperature can have a substantial effect on disease burden affecting human health. We examined the relationship between temperature andSalmonellaspp. and serotype notifications in Adelaide, Australia. Time-series Poisson regression models were fit to estimate the effect of temperature during warmer months onSalmonellaspp. and serotype cases notified from 1990 to 2012. Long-term trends, seasonality, autocorrelation and lagged effects were included in the statistical models. DailySalmonellaspp. counts increased by 1·3% [incidence rate ratio (IRR) 1·013, 95% confidence interval (CI) 1·008–1·019] per 1 °C rise in temperature in the warm season with greater increases observed in specific serotype and phage-type cases ranging from 3·4% (IRR 1·034, 95% CI 1·008–1·061) to 4·4% (IRR 1·044, 95% CI 1·024–1·064). We observed increased cases ofS. Typhimurium PT9 andS. Typhimurium PT108 notifications above a threshold of 39 °C. This study has identified the impact of warm season temperature on differentSalmonellaspp. strains and confirms higher temperature has a greater effect on phage-type notifications. The findings will contribute targeted information for public health policy interventions, including food safety programmes during warmer weather.

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Insulin biosynthesis in the bullhead, Ictalurus nebulosus, and the effect of temperature

Biochemical Journal ◽

10.1042/bj1340753 ◽

1973 ◽

Vol 134 (3) ◽

pp. 753-761 ◽

Cited By ~ 14

Author(s):

Margaret L. Moule ◽

Cecil C. Yip

Keyword(s):

Qualitative Difference ◽

Gel Filtration ◽

Effect Of Temperature ◽

Insulin Biosynthesis ◽

Temperature Sensitive ◽

Bicarbonate Buffer ◽

Control Tissue ◽

Ictalurus Nebulosus ◽

Lower Temperature

Insulin biosynthesis in the brown bullhead, Ictalurus nebulosus (Le Sueur), was studied by measuring the incorporation in vitro of [3H]leucine into proteins of the principal islet. The tissue was incubated for 6–15h in Krebs–Ringer bicarbonate buffer with [3H]leucine, supplemented with amino acids and glucose. Proteins, precipitated with trichloroacetic acid and extracted with acid ethanol, were separated by gel-filtration on Biogel P-30 in 3m-acetic acid. Three major components were found after incubation of the islets at 22°C. On the basis of the results of sulphitolysis, biological activity and the demonstrated precursor–product relationship, components I and II were identified as proinsulin and insulin respectively. The third component was not identified. At 12°C, [3H]leucine was incorporated only into proinsulin. No radioactivity was found in insulin or the unidentified component III at 12°C as was found after incubation at 22°C. When the temperature was lowered from 22° to 12°C after 3h of a 15h incubation, decreased conversion of proinsulin into insulin resulted at the lower temperature compared with the control tissue maintained at 22°C. When the temperature was raised from 12° to 22°C at 3h of a 15h incubation, conversion of proinsulin into insulin occurred. No conversion occurred in the control tissue with the temperature maintained at 12°C. No qualitative difference in the incorporation of [3H]leucine into proinsulin and its conversion into insulin at 12° and 22°C could be demonstrated between islet tissue from fish acclimated to less than 12°C or to 22°C. The results suggest that the enzyme(s) responsible for converting proinsulin into insulin in the bullhead may be temperature sensitive with low activity at 12°C.

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Computational Models for High-Temperature Multilayered Composite Plates and Shells

Applied Mechanics Reviews ◽

10.1115/1.3119742 ◽

1992 ◽

Vol 45 (10) ◽

pp. 419-446 ◽

Cited By ~ 184

Author(s):

Ahmed K. Noor ◽

W. Scott Burton

Keyword(s):

High Temperature ◽

Computational Models ◽

Thermal Stresses ◽

Three Dimensional ◽

Composite Plates ◽

Effect Of Temperature ◽

Temperature Sensitive ◽

Composite Models ◽

Sandwich Plates And Shells ◽

Plates And Shells

The focus of this review is on the hierarchy of composite models, predictor-corrector procedures, the effect of temperature-dependence of material properties on the response, and the sensitivity of the thermomechanical response to variations in material parameters. The literature reviewed is devoted to the following eight application areas: Heat transfer; thermal stresses; curing, processing and residual stresses; bifurcation buckling; vibrations of heated plates and shells; large deflection and postbuckling problems; and sandwich plates and shells. Extensive numerical results are presented showing the effects of variation in the lamination and geometric parameters of temperature-sensitive angle-ply composite plates on the accuracy of thermal buckling response, and the sensitivity derivatives predicted by nine different modeling approaches (based on two-dimensional theories). The standard of comparison is taken to be the exact three-dimensional thermoelasticity solutions. Some future directions for research on the modeling of high-temperature multilayered composites are outlined.

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