Detecting Nitrogen and Phosphorus Stress in Corn Using Multi-spectral Imagery

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is an excellent fast-growing timber species and has significant value in the forestry industry. In order to increase the nitrogen and phosphorus absorption and utilization in Chinese fir, shared differentially expressed genes under low nitrogen and phosphorus stress were screened in this study. Seedling of Chinese fir clone X6 was cultivated in aeroponic system with 3 treatments, namely, low nitrogen (LN), low phosphorus (LP) and a control check (with nitrogen and phosphorus sufficient, CK). After 4 months of treatment, the roots from the LN, LP and CK groups were collected and transcriptome sequencing was done by LC Sciences (USA) using an Illumina platform. When comparing the LN stress group with the CK group, 977 SDGEs were detected, 264 of which had KEGG annotations; 931 SDGEs were detected when comparing the LP stress group with the CK group, of which 189 had KEGG annotations; 297 SDGEs were detected in both the LN stress and LP stress groups, 78 of which had KEGG annotations representing 98 metabolic pathways. Among the 78 selected SDEGs that were differentially expressed under both LN and LP stress conditions, Twenty-one SDEGs were selected based on the metabolic pathways that nitrogen and phosphorus are involved in, these genes are PNR, PSBA, EGLC, GLC, END, BGLU, AMY, A1E, PAL, GOGAT, NIR1, NIR2, C4M, PAL, PRDX6, POX, CCR, CCoAOMT, FDH, CHS and ANR. These genes can potentially be used in breeding to improve both nitrogen and phosphorus utilization efficiency in Chinese fir.

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EFFECTS OF NITROGEN AND PHOSPHORUS STRESS ON THE GROWTH OF BOTRYOCOCCUS BRAUNII

Acta Hydrobiologica Sinica ◽

10.3724/sp.j.1035.2010.00517 ◽

2010 ◽

Vol 36 (3) ◽

pp. 517-524

Author(s):

Kai-Feng SUN ◽

Zhang-Xi HU ◽

Shun-Shan DUAN

Keyword(s):

Botryococcus Braunii ◽

Nitrogen And Phosphorus ◽

Phosphorus Stress

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Penapisan Mikroalga Penghasil Karotenoid serta Studi Pengaruh Stres Nitrogen dan Fosfor terhadap Produksi B-Karoten pada Mikroalga Oocystis sp.

Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan ◽

10.15578/jpbkp.v14i1.598 ◽

2019 ◽

Vol 14 (1) ◽

pp. 9

Author(s):

Wahida Nia Elfiza ◽

Abdi Dharma ◽

Nasril Nasir

Keyword(s):

Chlorophyll A ◽

Growth Medium ◽

Biomass Productivity ◽

Dry Weight ◽

Chlorophyll B ◽

Nitrogen And Phosphorus ◽

Total Carotenoids ◽

Screening Process ◽

Phosphorus Stress ◽

West Sumatra

Abstrakb-karoten merupakan karotenoid yang bermanfaat sebagai antioksidan. Penelitian ini bertujuan menapis mikroalga yang berpotensi mengandung karotenoid tinggi dan mempelajari pengaruh nitrogen dan fosfor terhadap produktivitas biomassa, kandungan pigmen fotosintesis dan b-karoten, pada mikroalga yang diisolasi dari perairan Danau Atas, Sumatra Barat. Penapisan mikroalga penghasil karotenoid dilakukan dengan memberikan paparan UV-A 326 nm terhadap kultur campuran mikroalga. Hasil penelitian menunjukkan bahwa 5 dari 18 spesies mampu bertahan pada proses penapisan. Pengaruh 9 jenis medium pertumbuhan dengan kriteria: tanpa NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, Bold Basalt Medium (BBM) normal (kontrol), tanpa KH2PO4, 3x KH2PO4, 5x KH2PO4,dan10x KH2PO4 terhadap mikroalga terpilih (Oocystis sp.) diamati. Hasil menunjukkan kandungan b-karoten tertinggi ditemukan pada perlakuan 5x KH2PO4 yaitu sebesar 0,22 % dari berat kering mikroalga, dengan produktivitas biomassa 0,0015 g/mL/hari, serta kandungan klorofil a, klorofil b dan karotenoid total yaitu 7,15 µg/mL, 0,81 µg/mL dan 6,67 µg/mL. Berdasarkan penelitian ini dapat disimpulkan medium pertumbuhan dengan kandungan 5x KH2PO4 merupakan medium yang cocok bagi pertumbuhan Oocystis sp. untuk mendapatkan biomassa dengan kandungan b-karoten dan karotenoid tinggi tanpa harus menurunkan produktivitas biomassanya. Screening of Carotenoid Producing Microalgae and Study of the Effect of Nitrogen and Phosphorus Stress on the Production of b-Carotene in Microalgae Oocystis sp.Abstractb-carotene is a carotenoid that is useful as an antioxidant. Present study aims to screening high microalgae which contain carotenoids from water of Danau Atas lake (West Sumatra Province), and study the effect of nitrogen and phosphorus on biomass productivity, photosynthetic and b-carotene pigment content. Screening of carotenoid-producing microalgae was carried out by exposing UV-A 326 nm to mixed microalgae cultures. The results showed that 5 of the 18 species were able to survive in the screening process. The effect of growth medium, i.e. without NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, normal Bold Basalt Medium (BBM) (control), without KH2PO4, 3x KH2PO4, 5x KH2PO4, and 10x KH2PO4 on of selected microalgae (Oocystis sp.) was carried out. The result showed that the highest content of b-carotene of Oocystis sp. was obtained with 5x KH2PO4 which was 0.22% of the dry weight of biomass. Biomass productivity was 0.0015 g/mL/day and the chlorophyll a, chlorophyll b and total carotenoids contents were 7.15 µg/mL, 0.81 µg/mL and 6.67 µg/mL, respectively. Based on this research, 5x KH2PO4 can be concluded as a suitable medium for Oocystis sp. to obtained high b -carotene and carotenoid of Oocystis sp. without reducing biomass productivity.

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Analysis of Metabolomic Changes in Lettuce Leaves under Low Nitrogen and Phosphorus Deficiencies Stresses

Agriculture ◽

10.3390/agriculture10090406 ◽

2020 ◽

Vol 10 (9) ◽

pp. 406 ◽

Cited By ~ 1

Author(s):

Hongyan Gao ◽

Hanping Mao ◽

Ikram Ullah

Keyword(s):

Fold Change ◽

Nitrogen Stress ◽

P Value ◽

Nitrogen And Phosphorus ◽

Phosphorus Stress ◽

Low Phosphorus ◽

Low Nitrogen Stress ◽

Low Phosphorus Stress ◽

Metabolic Biomarkers ◽

Low Nitrogen

Nitrogen and phosphorus limitation affect the growth, development, and productivity of lettuce, which exert a marked influence on metabolites. To compare the influence of low-nitrogen and low-phosphorus stresses on various metabolites of lettuce leaves, experiments were performed under three conditions of treatment—low-nitrogen stress, low-phosphorus stress, and normal samples. Metabolomic analyses were conducted based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Principle components analysis yielded distinctive clustering information among the holistic samples; fold change analysis, t-test and orthogonal partial least square discriminant analysis were used for the selection of metabolic biomarkers. Ten pathways were selected which were significantly enriched by metabolic biomarkers. Metabolic biomarkers were screened by fold change (FC) value, p-value and variable importance in the projection (VIP) value, low-nitrogen and low-phosphorus stresses caused an increase in 16 metabolites (FC > 2, p-value < 0.05, VIP > 1) and a decrease in 26 metabolites (FC < 0.5, p-value < 0.05, VIP > 1). Outside of these, our results showed that inositol, p-hydroxybenzoic acid, stachyose, dinoseb, and 7, 8-dihydroxycoumarin increase in low-nitrogen stress samples. Low-phosphorus stress caused accumulation of citrate, isocitrate, l-5-oxoproline, succinate, and histamine, which may be considered potential metabolic biomarkers. The metabolites could be used to monitor the nitrogen and phosphorus status of lettuce and to guide appropriate fertilization regimens.

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Genome-Wide Selection for tropical maize root traits under conditions of nitrogen and phosphorus stress

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v34i4.15884 ◽

2012 ◽

Vol 34 (4) ◽

Cited By ~ 5

Author(s):

Roberto Fritsche-Neto ◽

Júlio César DoVale ◽

Éder Cristian Malta de Lanes ◽

Marcos Deon Vilela de Resende ◽

Glauco Vieira Miranda

Keyword(s):

Root Traits ◽

Maize Root ◽

Tropical Maize ◽

Nitrogen And Phosphorus ◽

Phosphorus Stress ◽

Genome Wide ◽

Selection For

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Shared differentially expressed genes of Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) seedlings under low nitrogen and phosphorus stress

10.7287/peerj.preprints.2916v1 ◽

2017 ◽

Author(s):

JianHui Li ◽

GuiFang Ma ◽

DingWei Luo ◽

ZaiKang Tong ◽

Jinliang Xu ◽

...

Keyword(s):

Differentially Expressed Genes ◽

Metabolic Pathways ◽

Chinese Fir ◽

Cunninghamia Lanceolata ◽

Utilization Efficiency ◽

Differentially Expressed ◽

Nitrogen And Phosphorus ◽

Stress Group ◽

Phosphorus Stress ◽

Low Nitrogen

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is an excellent fast-growing timber species and has significant value in the forestry industry. In order to increase the nitrogen and phosphorus absorption and utilization in Chinese fir, shared differentially expressed genes under low nitrogen and phosphorus stress were screened in this study. Seedling of Chinese fir clone X6 was cultivated in aeroponic system with 3 treatments, namely, low nitrogen (LN), low phosphorus (LP) and a control check (with nitrogen and phosphorus sufficient, CK). After 4 months of treatment, the roots from the LN, LP and CK groups were collected and transcriptome sequencing was done by LC Sciences (USA) using an Illumina platform. When comparing the LN stress group with the CK group, 977 SDGEs were detected, 264 of which had KEGG annotations; 931 SDGEs were detected when comparing the LP stress group with the CK group, of which 189 had KEGG annotations; 297 SDGEs were detected in both the LN stress and LP stress groups, 78 of which had KEGG annotations representing 98 metabolic pathways. Among the 78 selected SDEGs that were differentially expressed under both LN and LP stress conditions, Twenty-one SDEGs were selected based on the metabolic pathways that nitrogen and phosphorus are involved in, these genes are PNR, PSBA, EGLC, GLC, END, BGLU, AMY, A1E, PAL, GOGAT, NIR1, NIR2, C4M, PAL, PRDX6, POX, CCR, CCoAOMT, FDH, CHS and ANR. These genes can potentially be used in breeding to improve both nitrogen and phosphorus utilization efficiency in Chinese fir.

Download Full-text