scholarly journals Unique distribution of the extracellular matrix component thrombospondin in the developing mouse embryo.

1988 ◽  
Vol 107 (6) ◽  
pp. 2737-2748 ◽  
Author(s):  
K S O'Shea ◽  
V M Dixit
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Mouse Embryo ◽  
Basement Membranes ◽  
Extracellular Matrices ◽  
Immunocytochemical Localization ◽  
Extracellular Matrix Component ◽  
Cortical Layers ◽  
Matrix Component ◽  
Unique Distribution

Immunocytochemical localization of thrombospondin (TSP), a trimeric glycoprotein constituent of extracellular matrices, produced striking regional and temporal patterns of distribution in the developing mouse embryo. TSP was present in many basement membranes, surrounded epithelial cells, and was associated with peripheral nerve outgrowth. During organogenesis, TSP was also found on the surface of myoblasts and chondroblasts, and TSP was differentially deposited in cortical layers. With differentiation of chondrocytes and myotubes immunoreactivity was decreased, and differential cortical staining was lost. Presence of TSP was associated with morphogenetic processes of proliferation, migration, and intercellular adhesion.

scholarly journals Evidence of Swim secretion and association with extracellular matrix in the Drosophila embryo

2021 ◽  
Author(s):  
Valeria Kaltezioti ◽  
Katerina M. Vakaloglou ◽  
Aristidis S. Charonis ◽  
Christos G. Zervas
Keyword(s):  
Extracellular Matrix ◽  
Expression System ◽  
Ectopic Expression ◽  
Basement Membranes ◽  
Confocal Imaging ◽  
Drosophila Embryo ◽  
Dependent Manner ◽  
Extracellular Matrix Component ◽  
Matrix Component

Secreted wingless-interacting protein (Swim) is the Drosophila ortholog gene of the mammalian Tubulointerstitial Nephritis Antigen Like 1 (TINAGL1), known also as lipocalin-7 (LCN7), or adrenocortical zonation factor 1 (AZ-1). Swim and TINAGL1 proteins share a significant homology, including the somatomedin B and the predictive inactive C1 cysteine peptidase domains. In mammals, both TINAGL1 and its closely related homolog TINAG have been identified in basement membranes, where they may function as modulators of integrin-mediated adhesion. In Drosophila, Swim was initially identified in the eggshell matrix and subsequently was detected in the culture medium of S2 cells. Further biochemical analysis indicated that Swim binds to wingless (wg) in a lipid-dependent manner. This observation together with RNAiknockdown studies suggested that Swim is an essential cofactor of wg-signalling. However, recent elegant genetic studies ruled out the possibility that Swim is required alone to facilitate wgsignalling in Drosophila, because flies without Swim are viable and fertile. Here, we use the UAS/Gal4 expression system together with confocal imaging to analyze the in vivo localization of a chimeric Swim-GFP in the developing Drosophila embryo. Our data fully support the notion that Swim is an extracellular matrix component that upon ectopic expression is secreted and preferentially associates with the basement membranes of various organs and with the specialized tendon matrix at the muscle attachment sites (MAS). Interestingly, the accumulation of Swim at the MAS does not require integrins. In conclusion, Swim is an extracellular matrix component, and it is possible that Swim exhibits overlapping functions in concert with other undefined components.

scholarly journals Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves

2021 ◽  
Vol 22 (5) ◽  
pp. 2389
Author(s):  
Marwa El El Soury ◽  
Óscar Darío García-García ◽  
Matteo Moretti ◽  
Isabelle Perroteau ◽  
Stefania Raimondo ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Peripheral Nerve Regeneration ◽  
Extracellular Matrix Component ◽  
Matrix Component ◽  
Viability Assay ◽  
In Vitro Characterization ◽  
Sciatic Nerves

In critical nerve gap repair, decellularized nerve allografts are considered a promising tissue engineering strategy that can provide superior regeneration results compared to nerve conduits. Decellularized nerves offer a well-conserved extracellular matrix component that has proven to play an important role in supporting axonal guiding and peripheral nerve regeneration. Up to now, the known decellularized techniques are time and effort consuming. The present study, performed on rat sciatic nerves, aims at investigating a novel nerve decellularization protocol able to combine an effective decellularization in short time with a good preservation of the extracellular matrix component. To do this, a decellularization protocol proven to be efficient for tendons (DN-P1) was compared with a decellularization protocol specifically developed for nerves (DN-P2). The outcomes of both the decellularization protocols were assessed by a series of in vitro evaluations, including qualitative and quantitative histological and immunohistochemical analyses, DNA quantification, SEM and TEM ultrastructural analyses, mechanical testing, and viability assay. The overall results showed that DN-P1 could provide promising results if tested in vivo, as the in vitro characterization demonstrated that DN-P1 conserved a better ultrastructure and ECM components compared to DN-P2. Most importantly, DN-P1 was shown to be highly biocompatible, supporting a greater number of viable metabolically active cells.

scholarly journals Hyperglycaemia and aberrated insulin signalling stimulate tumour progression via induction of the extracellular matrix component hyaluronan

2017 ◽  
Vol 141 (4) ◽  
pp. 791-804 ◽  
Author(s):  
Sören Twarock ◽  
Christina Reichert ◽  
Ulrike Peters ◽  
Daniel J. Gorski ◽  
Katharina Röck ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Insulin Signalling ◽  
Tumour Progression ◽  
Extracellular Matrix Component ◽  
Matrix Component

The impact of age on cardiac function and extracellular matrix component expression in adverse post-infarction remodeling in mice

2019 ◽  
Vol 119 ◽  
pp. 193-202 ◽  
Author(s):  
Felix Nagel ◽  
David Santer ◽  
Stefan Stojkovic ◽  
Christoph Kaun ◽  
Anne-Kristin Schaefer ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cardiac Function ◽  
Extracellular Matrix Component ◽  
Matrix Component ◽  
The Impact

scholarly journals Ultrastructural localization of the major components of the extracellular matrix in normal rat nerve.

1992 ◽  
Vol 40 (6) ◽  
pp. 859-868 ◽  
Author(s):  
P Lorimier ◽  
P Mezin ◽  
F Labat Moleur ◽  
N Pinel ◽  
S Peyrol ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Ultrastructural Localization ◽  
Basement Membranes ◽  
Type I ◽  
Immunoperoxidase Method ◽  
Adult Rat ◽  
Normal Rat ◽  
Rat Nerve ◽  
Fibronectin Type

In this study we determined the ultrastructural distribution of the various components of the extracellular matrix (laminin, fibronectin, Type I, III, and IV collagens) of the normal peripheral nerve in adult rat. The localization of these macromolecules was investigated in basement membranes as well as in different areas of epi-, peri-, and endoneurium, by use of a pre-embedding immunoperoxidase method.

scholarly journals Cartilage link protein 1 (Crtl1), an extracellular matrix component playing an important role in heart development

2007 ◽  
Vol 310 (2) ◽  
pp. 291-303 ◽  
Author(s):  
Elaine E. Wirrig ◽  
Brian S. Snarr ◽  
Mastan R. Chintalapudi ◽  
Jessica L. O'Neal ◽  
Aimee L. Phelps ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Heart Development ◽  
Extracellular Matrix Component ◽  
Matrix Component ◽  
Link Protein
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