scholarly journals Characterization of actin filament severing by actophorin from Acanthamoeba castellanii.

1991 ◽  
Vol 115 (6) ◽  
pp. 1611-1620 ◽  
Author(s):  
S K Maciver ◽  
H G Zot ◽  
T D Pollard
Keyword(s):  
Steady State ◽  
Actin Filament ◽  
Polymer Concentration ◽  
Acanthamoeba Castellanii ◽  
Homologous Proteins ◽  
Spontaneous Polymerization ◽  
Mammalian Tissues ◽  
Covalent Complex ◽  
Low Shear

Actophorin is an abundant 15-kD actinbinding protein from Acanthamoeba that is thought to form a nonpolymerizable complex with actin monomers and also to reduce the viscosity of polymerized actin by severing filaments (Cooper et al., 1986. J. Biol. Chem. 261:477-485). Homologous proteins have been identified in sea urchin, chicken, and mammalian tissues. Chemical crosslinking produces a 1:1 covalent complex of actin and actophorin. Actophorin and profilin compete for crosslinking to actin monomers. The influence of actophorin on the steady-state actin polymer concentration gave a Kd of 0.2 microM for the complex of actophorin with actin monomers. Several new lines of evidence, including assays for actin filament ends by elongation rate and depolymerization rate, show that actophorin severs actin filaments both at steady state and during spontaneous polymerization. This is confirmed by direct observation in the light microscope and by showing that the effects of actophorin on the low shear viscosity of polymerized actin cannot be explained by monomer sequestration. The severing activity of actophorin is strongly inhibited by stoichiometric concentrations of phalloidin or millimolar concentrations of inorganic phosphate.

scholarly journals Isolation and partial characterization of a 110-kD dimer actin-binding protein.

1986 ◽  
Vol 103 (2) ◽  
pp. 621-630 ◽  
Author(s):  
T Ueno ◽  
E D Korn
Keyword(s):  
Myosin Ii ◽  
Deae Cellulose ◽  
Acanthamoeba Castellanii ◽  
Actin Binding ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Myosin I ◽  
Soluble Supernatant ◽  
Low Shear

Two Triton-insoluble fractions were isolated from Acanthamoeba castellanii. The major non-membrane proteins in both fractions were actin (30-40%), myosin II (4-9%), myosin I (1-5%), and a 55-kD polypeptide (10%). The 55-kD polypeptide did not react with antibodies against tubulins from turkey brain, paramecium, or yeast. All of these proteins were much more concentrated in the Triton-insoluble fractions than in the whole homogenate or soluble supernatant. The 55-kD polypeptide was extracted with 0.3 M NaCl, fractionated by ammonium sulfate, and purified to near homogeneity by DEAE-cellulose and hydroxyapatite chromatography. The purified protein had a molecular mass of 110 kD and appeared to be a homodimer by isoelectric focusing. The 110-kD dimer bound to F-actin with a maximal binding stoichiometry of 0.5 mol/mol of actin (1 mol of 55-kD subunit/mol of actin). Although the 110-kD protein enhanced the sedimentation of F-actin, it did not affect the low shear viscosity of F-actin solutions nor was bundling of F-actin observed by electron microscopy. The 110-kD dimer protein inhibited the actin-activated Mg2+-ATPase activities of Acanthamoeba myosin I and myosin II in a concentration-dependent manner. By indirect immunofluorescence, the 110-kD protein was found to be localized in the peripheral cytoplasm near the plasma membrane which is also enriched in F-actin filaments and myosin I.

Fabrication, Optimization and Characterization of Paclitaxel and Spirulina Loaded Nanoparticles for Enhanced Oral Bioavailability

2020 ◽  
Vol 16 (5) ◽  
pp. 723-733
Author(s):  
Keerthi G.S. Nair ◽  
Yamuna Ravikumar ◽  
Sathesh Kumar Sukumaran ◽  
Ramaiyan Velmurugan
Keyword(s):  
Gastric Cancer ◽  
Oral Bioavailability ◽  
Surfactant Concentration ◽  
Polymer Concentration ◽  
Central Composite Rotatable Design ◽  
Normal Cells ◽  
Stirring Time ◽  
Nanoparticle Formulation ◽  
Central Composite

Background: Paclitaxel and spirulina when administered as nanoparticles, are potentially useful. Methods: Nanoformualtions of Paclitaxel and Spirulina for gastric cancer were formulated and optimized with Central composite rotatable design (CCRD) using Response surface methodology (RSM). Results: The significant findings were the optimal formulation of polymer concentration 48 mg, surfactant concentration 45% and stirring time of 60 min gave rise to the EE of (98.12 ± 1.3)%, DL of (15.61 ± 1.9)%, mean diameter of (198 ± 4.7) nm. The release of paclitaxel and spirulina from the nanoparticle matrix at pH 6.2 was almost 45% and 80% in 5 h and 120 h, respectively. The oral bioavailability for the paclitaxel spirulina nanoparticles developed is 24.0% at 10 mg/kg paclitaxel dose, which is 10 times of that for oral pure paclitaxel. The results suggest that RSM-CCRD could efficiently be applied for the modeling of nanoparticles. The paclitaxel and spirulina release rate in the tumor cells may be higher than in normal cells. Paclitaxel spirulina nanoparticle formulation may have higher bioavailability and longer sustainable therapeutic time as compared with pure paclitaxel. Conclusion: Paclitaxel-Spirulina co-loaded nanoparticles could be effectively useful in gastric cancer as chemotherapeutic formulation.

scholarly journals Fabrication and Characterization of Electrospun Poly(acrylonitrile-co-Methyl Acrylate)/Lignin Nanofibers: Effects of Lignin Type and Total Polymer Concentration

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 992
Author(s):  
Suchitha Devadas ◽  
Saja M. Nabat Al-Ajrash ◽  
Donald A. Klosterman ◽  
Kenya M. Crosson ◽  
Garry S. Crosson ◽  
...  
Keyword(s):  
Methyl Acrylate ◽  
Lignin Content ◽  
Polymer Concentration ◽  
Weight Ratio ◽  
Electrospun Nanofibers ◽  
Polymer Content ◽  
Blend Ratio ◽  
Blend Ratios ◽  
Poly Acrylonitrile

Lignin macromolecules are potential precursor materials for producing electrospun nanofibers for composite applications. However, little is known about the effect of lignin type and blend ratios with synthetic polymers. This study analyzed blends of poly(acrylonitrile-co-methyl acrylate) (PAN-MA) with two types of commercially available lignin, low sulfonate (LSL) and alkali, kraft lignin (AL), in DMF solvent. The electrospinning and polymer blend solution conditions were optimized to produce thermally stable, smooth lignin-based nanofibers with total polymer content of up to 20 wt % in solution and a 50/50 blend weight ratio. Microscopy studies revealed that AL blends possess good solubility, miscibility, and dispersibility compared to LSL blends. Despite the lignin content or type, rheological studies demonstrated that PAN-MA concentration in solution dictated the blend’s viscosity. Smooth electrospun nanofibers were fabricated using AL depending upon the total polymer content and blend ratio. AL’s addition to PAN-MA did not affect the glass transition or degradation temperatures of the nanofibers compared to neat PAN-MA. We confirmed the presence of each lignin type within PAN-MA nanofibers through infrared spectroscopy. PAN-MA/AL nanofibers possessed similar morphological and thermal properties as PAN-MA; thus, these lignin-based nanofibers can replace PAN in future applications, including production of carbon fibers and supercapacitors.

scholarly journals Characterization of a second myosin from Acanthamoeba castellanii

1978 ◽  
Vol 253 (13) ◽  
pp. 4798-4808 ◽  
Author(s):  
T.D. Pollard ◽  
W.F. Stafford ◽  
M.E. Porter
Keyword(s):  
Acanthamoeba Castellanii

Steady-state Characterization of LLC-based Single-Stage AC/DC Converter Based on Numerical Analysis

2021 ◽  
pp. 1-1
Author(s):  
Xiang Li ◽  
Liuniu Guo ◽  
Tianchen Lang ◽  
Daorong Lu ◽  
Khalil Alluhaybi ◽  
...  
Keyword(s):  
Steady State ◽  
Numerical Analysis ◽  
Single Stage

Steady-State Parametric Optimization and Transient Characterization of Heat Flow Regulation With Binary Diffusion

2020 ◽  
Vol 10 (12) ◽  
pp. 1996-2007
Author(s):  
Tanya Liu ◽  
James W. Palko ◽  
Joseph S. Katz ◽  
Feng Zhou ◽  
Ercan M. Dede ◽  
...  
Keyword(s):  
Steady State ◽  
Heat Flow ◽  
Parametric Optimization ◽  
Flow Regulation ◽  
Binary Diffusion

scholarly journals Characterization of the nonequilibrium steady state of a heterogeneous nonlinear q-voter model with zealotry

2016 ◽  
Vol 113 (4) ◽  
pp. 48001 ◽  
Author(s):  
Andrew Mellor ◽  
Mauro Mobilia ◽  
R. K. P. Zia
Keyword(s):  
Steady State ◽  
Nonequilibrium Steady State ◽  
Voter Model
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