scholarly journals Nuclear translation visualized by ribosome-bound nascent chain puromycylation

2012 ◽  
Vol 197 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Alexandre David ◽  
Brian P. Dolan ◽  
Heather D. Hickman ◽  
Jonathan J. Knowlton ◽  
Giovanna Clavarino ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunofluorescence Microscopy ◽  
Protein Translation ◽  
Chain Elongation ◽  
Chemical Stress ◽  
Specific Monoclonal Antibody ◽  
Permeabilized Cells ◽  
Nascent Chain ◽  
Nuclear Translation ◽  
In Cells

Whether protein translation occurs in the nucleus is contentious. To address this question, we developed the ribopuromycylation method (RPM), which visualizes translation in cells via standard immunofluorescence microscopy. The RPM is based on ribosome-catalyzed puromycylation of nascent chains immobilized on ribosomes by antibiotic chain elongation inhibitors followed by detection of puromycylated ribosome-bound nascent chains with a puromycin (PMY)-specific monoclonal antibody in fixed and permeabilized cells. The RPM correlates localized translation with myriad processes in cells and can be applied to any cell whose translation is sensitive to PMY. In this paper, we use the RPM to provide evidence for translation in the nucleoplasm and nucleolus, which is regulated by infectious and chemical stress.

Therapeutic Potential of SARS-CoV-2-Specific Monoclonal Antibody CR3022

2020 ◽  
Author(s):  
Caroline Atyeo ◽  
Matthew D. Slein ◽  
Stephanie Fischinger ◽  
John Burke ◽  
Alexandra Schӓfer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Therapeutic Potential ◽  
Specific Monoclonal Antibody

SIRPα-specific monoclonal antibody enables antibody-dependent phagocytosis of neuroblastoma cells

2021 ◽  
Author(s):  
Meriem Bahri ◽  
Sareetha Kailayangiri ◽  
Sarah Vermeulen ◽  
Natacha Galopin ◽  
Claudia Rossig ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Neuroblastoma Cells ◽  
Specific Monoclonal Antibody

scholarly journals A new reliable and highly specific monoclonal antibody to detect the C‐terminal region of Silencer of Cytokine Signaling 1 (SOCS1)

2021 ◽  
Author(s):  
Stephanie E. Weissinger ◽  
Malena Zahn ◽  
Ralf Marienfeld ◽  
Claudia Tessmer ◽  
Gerhard Moldenhauer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Terminal Region ◽  
Cytokine Signaling ◽  
Specific Monoclonal Antibody

scholarly journals Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein

2021 ◽  
Vol 22 (4) ◽  
pp. 2001
Author(s):  
Silvia Spena ◽  
Chiara Cordiglieri ◽  
Isabella Garagiola ◽  
Flora Peyvandi
Keyword(s):  
Monoclonal Antibody ◽  
Prenatal Diagnosis ◽  
Maternal Blood ◽  
Limiting Factors ◽  
Inherited Disease ◽  
Specific Monoclonal Antibody ◽  
Native Form ◽  
Fetal Cells ◽  
Non Invasive ◽  
Reliable Isolation

Hemophilia is an X-linked recessive bleeding disorder. In pregnant women carrier of hemophilia, the fetal sex can be determined by non-invasive analysis of fetal DNA circulating in the maternal blood. However, in case of a male fetus, conventional invasive procedures are required for the diagnosis of hemophilia. Fetal cells, circulating in the maternal bloodstream, are an ideal target for a safe non-invasive prenatal diagnosis. Nevertheless, the small number of cells and the lack of specific fetal markers have been the most limiting factors for their isolation. We aimed to develop monoclonal antibodies (mAbs) against the ribosomal protein RPS4Y1 expressed in male cells. By Western blotting, immunoprecipitation and immunofluorescence analyses performed on cell lysates from male human hepatoma (HepG2) and female human embryonic kidney (HEK293) we developed and characterized a specific monoclonal antibody against the native form of the male RPS4Y1 protein that can distinguish male from female cells. The availability of the RPS4Y1-targeting monoclonal antibody should facilitate the development of novel methods for the reliable isolation of male fetal cells from the maternal blood and their future use for non-invasive prenatal diagnosis of X-linked inherited disease such as hemophilia.

scholarly journals Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice

2007 ◽  
Vol 83 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Jean M. Daley ◽  
Alan A. Thomay ◽  
Michael D. Connolly ◽  
Jonathan S. Reichner ◽  
Jorge E. Albina
Keyword(s):  
Monoclonal Antibody ◽  
Specific Monoclonal Antibody

Cell surface detection of HLA-E gene products with a specific monoclonal antibody

1999 ◽  
Vol 43 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Rita Pacasova ◽  
Silvia Martinozzi ◽  
Henri-Jean Boulouis ◽  
Yann Szpak ◽  
Matthias Ulbrecht ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cell Surface ◽  
Gene Products ◽  
Specific Monoclonal Antibody ◽  
E Gene ◽  
Surface Detection
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