scholarly journals TISSUE-SPECIFIC CELL-SURFACE ANTIGENS IN EMBRYONIC CELLS

1972 ◽  
Vol 53 (2) ◽  
pp. 435-449 ◽  
Author(s):  
Irving Goldschneider ◽  
A. A. Moscona
Keyword(s):  
Cell Surface ◽  
Surface Antigens ◽  
Embryonic Cell ◽  
Specific Cell ◽  
Embryonic Chick ◽  
Embryonic Cells ◽  
Cell Surfaces ◽  
Cell Surface Antigens ◽  
Tissue Specific ◽  
High Degree

With the use of antisera prepared in rabbits against suspensions of live embryonic chick tissue cells, qualitative differences in cell surface antigens were demonstrated on cells from different embryonic chick tissues by immune agglutination and immunofluorescence. Unabsorbed antisera reacted with both homologous and nonhomologous cells; thorough absorption of the antisera with heterologous tissues removed cross-reacting antibodies, and the antisera acquired a high degree of tissue specificity. Thus, antiretina cell serum absorbed with nonretina cells or tissues, agglutinated only neural retina cells, and was shown by immunofluorescence tests to react specifically with the surface of retina cells, both in cell suspensions and in frozen tissue sections. Comparable results with antisera against cells from embryonic liver and other tissues demonstrated the existence of tissue-specific, phenotypic disparities in the antigenicities of embryonic cell surfaces, in addition to the presence of cell-surface antigens shared by certain classes of cells, and of antigens common to all cells in the embryo. The results are discussed in terms of the possible involvement of such phenotypic determinants in the specification of cell surfaces, in relation to cell recognition and developmental interactions.

Monoclonal antibodies to tissue-specific cell surface antigens

1984 ◽  
Vol 33 (2) ◽  
pp. 268-281 ◽  
Author(s):  
Ann M. Carroll ◽  
Michael Zalutsky ◽  
Sam Schatten ◽  
Atul Bhan ◽  
Linda L. Perry ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Surface Antigens ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Tissue Specific ◽  
Specific Cell Surface

scholarly journals Large-scale co-aggregation of fluorescent lipid probes with cell surface proteins.

1994 ◽  
Vol 125 (4) ◽  
pp. 795-802 ◽  
Author(s):  
J L Thomas ◽  
D Holowka ◽  
B Baird ◽  
W W Webb
Keyword(s):  
Cell Surface ◽  
Large Scale ◽  
Plasma Membranes ◽  
Immunoglobulin E ◽  
Large Fraction ◽  
Surface Antigens ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Receptor Complexes ◽  
Ige Receptors

Large scale aggregation of fluorescein-labeled immunoglobulin E (IgE) receptor complexes on the surface of RBL cells results in the co-aggregation of a large fraction of the lipophilic fluorescent probe 3,3'-dihexadecylindocarbocyanine (diI) that labels the plasma membranes much more uniformly in the absence of receptor aggregation. Most of the diI molecules that are localized in patches of aggregated receptors have lost their lateral mobility as determined by fluorescence photobleaching recovery. The diI outside of patches is mobile, and its mobility is similar to that in control cells without receptor aggregates. It is unlikely that the co-aggregation of diI with IgE receptors is due to specific interactions between these components, as two other lipophilic probes of different structures are also observed to redistribute with aggregated IgE receptors, and aggregation of two other cell surface antigens also results in the coredistribution of diI at the RBL cell surface. Quantitative analysis of CCD images of labeled cells reveals some differences in the spatial distributions of co-aggregated diI and IgE receptors. The results indicate that cross-linking of specific cell surface antigens causes a substantial change in the organization of the plasma membrane by redistributing pre-existing membrane domains or causing their formation.

Cell surface antigens in early mammalian development

1983 ◽  
Vol 41 ◽  
pp. 584-587
Author(s):  
Lincoln V. Johnson ◽  
Patricia G. Calarco ◽  
Syivia Ploack-Charcon
Keyword(s):  
Cell Surface ◽  
Specific Cell ◽  
Cell Surface Molecules ◽  
Embryonic Cells ◽  
Mammalian Development ◽  
Mouse Development ◽  
Cell Surface Antigens ◽  
Surface Molecules ◽  
Preimplantation Mouse ◽  
Or Organization

Cell surface molecules are known to be functionally important In a number of developmental systems. Molecular components of the cell surface may participate in Intercellular adhesion, recognition and communication. Reactions occur r Ing at the cell surface may also influence developmental events by Induci ng i ntracelIular changes In cellular metabolism and gene expression which may, In turn, alter the composition and/or organization of surface components. Sequences of reactions mediated by cell surface molecules are probably Important In directing undifferentiated embryonic cells along specific dlfferentlatlve pathways.In the early development of the mammal Ian embryo, important events occurring during the preimplantation period are likely to require the participation of specific cell surface molecules. For example, these are likely to Include sperm-egg attachment and fusion, Interbl astomere adhesion, blastulatlon, and attachment of the blastocyst to the uterine epithelium. During preimplantation mouse development, specific stage-relaged changes in the qual itatlve and quantitative nature of the proteins synthesized and those expressed on the cell surface have been documented.

Identification of New Multiple Myeloma-Specific Cell Surface Antigens As Immunotherapeutic Targets

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 3252-3252
Author(s):  
Naoki Hosen ◽  
Kana Hasegawa ◽  
Yasutaka Aoyama ◽  
Hiroyoshi Ichihara ◽  
Atsuko Mugitani ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
T Cells ◽  
Cell Surface ◽  
Surface Antigens ◽  
Expression Cloning ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Car T Cells ◽  
Car T ◽  
Specific Cell Surface

Abstract Cancer-specific cell surface antigens are ideal targets for therapies using monoclonal antibodies (mAbs) and their derivatives, such as chimeric antigen receptor (CAR)-T cells. However, such antigens are not likely to remain unidentified following extensive searching by transcriptome or proteome analyses. However, we hypothesized that cancer-specific antigens formed by post-translational events, such as glycosylation, complex formation, or conformational changes, might have been missed in previous screens. Such antigens could be discovered by thoroughly searching for cancer-specific mAbs and characterizing the antigens recognized by these mAbs. To test our hypothesis, we applied this strategy to identify novel therapeutic targets specific for multiple myeloma (MM), a major hematological cancer. We first identified two MM-specific mAbs designated as MMG49 or R8H283 after screening more than 10,000 anti-MM mAb clones. Then, we identified the antigens recognized by these mAbs by an expression cloning method. Interestingly, genes identified as antigens for both mAbs were not specific to MM cells, suggesting that both mAbs recognize MM-specific epitopes formed by post-translational events. Finally, we showed that these mAbs or CAR-T cells derived from them could reduce tumor burden in MM-xenograft models, but did not damage normal hematopoietic cells. These results not only demonstrate that these mAb or CAR-T cell therapy is promising for MM, but also suggest that MM-specific immunotherapetic target antigens formed by post translational events may be still missed. Disclosures Aoyama: Alexion: Honoraria.

Sign in / Sign up

Export Citation Format

Share Document